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The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface
M. Steegmaier, E. Borges, J. Berger, H. Schwarz, D. Vestweber
Journal of Cell Science 1997 110: 687-694;

Summary

Neutrophils and subsets of lymphocytes bind to E-selectin, a cytokine inducible adhesion molecule on endothelial cells. The E-selectin-ligand-1 (ESL-1) is a high affinity glycoprotein ligand which participates in the binding of mouse myeloid cells to E-selectin. The sequence of mouse ESL-1 is highly homologous to the cysteine rich FGF receptor (CFR) in chicken and the rat Golgi protein MG160. We have analysed the subcellular distribution of ESL-1 by indirect immunofluorescence, flow cytometry, various biochemical techniques and by immunogold scanning electron microscopy. We could localize ESL-1 in the Golgi as well as on the cell surface of 32Dc13 cells and neutrophils. Cell surface staining was confirmed by cell surface biotinylation and by cell surface immunoprecipitations in which antibodies only had access to surface proteins on intact cells. In addition, ESL-1(high) and ESL-1(low) expressing cells, sorted by flow cytometry, gave rise to high and low immunoprecipitation signals for ESL-1, respectively. Based on immunogold labeling of intact cells, we localized ESL-1 on microvilli of 32Dc13 cells and of the lymphoma cell line K46. Quantitative evaluation determined 80% of the total labeling for ESL-1 on microvilli of K46 cells while 69% of the labeling for the control antigen B220 was found on the planar cell surface. These data indicate that ESL-1 occurs at sites on the leukocyte cell surface which are destined for the initiation of cell contacts to the endothelium.

REFERENCES

    1. Autrata R.,
    2. Hermann R. and
    3. Muller M.
    (1992). An efficient single crystal BSE detector in SEM. Scanning 14, 127–135
    OpenUrl
    1. Baumhueter S.,
    2. Singer M. S.,
    3. Henzel W.,
    4. Hemmerich S.,
    5. Renz M.,
    6. Rosen S. D. and
    7. Lasky L. A.
    (1993). Binding of L-selectin to the vascular sialomucin CD34. Science 262, 436–438
    OpenUrlAbstract/FREE Full Text
    1. Berlin C.,
    2. Berg E. L.,
    3. Briskin M. J.,
    4. Andrew D.,
    5. Kilshaw P.,
    6. Holzmann B.,
    7. Weissman I. L.,
    8. Hamann A. and
    9. Butcher E. C.
    (1993). 4 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74, 185–195
    OpenUrlCrossRefPubMedWeb of Science
    1. Bosse R. and
    2. Vestweber D.
    (1994). Only simultaneous blocking of the L-and P-selectin completely inhibits neutrophil migration into mouse peritoneum. Eur. J. Immunol 24, 3019–3024
    OpenUrlCrossRefPubMedWeb of Science
    1. Bradley J. R.,
    2. Thiru S. and
    3. Pober J. S.
    (1995). Disparate localization of 55-kd and 75-kd tumor necrosis factor receptors in human endothelial cells. Am. J. Pathol 146, 27–32
    OpenUrlPubMedWeb of Science
    1. Butcher E. C.
    (1991). Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity. Cell 67, 1033–1036
    OpenUrlCrossRefPubMedWeb of Science
    1. Burrus L. W. and
    2. Olwin B. B.
    (1989). Isolation of a receptor for acidic and basic fibroblast growth factor from embryonic chick. J. Biol. Chem 264, 18647–18653
    OpenUrlAbstract/FREE Full Text
    1. Burrus L. W.,
    2. Zuber M. E.,
    3. Lueddecke B. A. and
    4. Olwin B. B.
    (1992). Identification of a cysteine-rich receptor for fibroblast growth factors. Mol. Cell. Biol 12, 5600–5609
    OpenUrlAbstract/FREE Full Text
    1. Erlandsen S. L.,
    2. Hasslen S. R. and
    3. Nelson R. D.
    (1993). Detection and spatial distribution of the2 integrin (Mac-1) and L-selectin (LECAM-1) adherence receptors on human neutrophils by high-resolution field emission SEM. J. Histochem. Cytochem 41, 327–333
    OpenUrlAbstract/FREE Full Text
    1. Gallatin W. M.,
    2. Weissman I. L. and
    3. Butcher E. C.
    (1983). A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 304, 30–34
    OpenUrlCrossRefPubMedWeb of Science
    1. Gonatas J. O.,
    2. Mezitis S. G. E.,
    3. Stieber A.,
    4. Fleischer B. and
    5. Gonatas N. K.
    (1989). MG-160: a novel sialoglycoprotein of the medial cisternae of the Golgi apparatus. J. Biol. Chem 264, 646–653
    OpenUrlAbstract/FREE Full Text
    1. Gonatas J. O.,
    2. Mourelatos Z.,
    3. Stieber A.,
    4. Lane W. S.,
    5. Brosius J. and
    6. Gonatas N. K.
    (1995). MG160, a membrane sialoglycoprotein of the medial cisternae of the rat Golgi apparatus, binds basic fibroblast growth factor and exhibits a high level of sequence identity to a chicken fibroblast growth factor receptor. J. Cell Sci 108, 457–467
    OpenUrlAbstract/FREE Full Text
    1. Hemmerich S. and
    2. Rosen S. D.
    (1994). 6-Sulfated sialyl Lewis × is a major capping group of GlyCAM-1. Biochemistry 33, 4830–4835
    OpenUrlCrossRefPubMed
    1. Imai Y.,
    2. Lasky L. A. and
    3. Rosen S. D.
    (1993). Sulphation requirement for GlyCAM-1, an endothelial ligand for L-selectin. Nature 361, 555–557
    OpenUrlCrossRefPubMedWeb of Science
    1. Kim K.-M.,
    2. Adachi T.,
    3. Nielsen P. J.,
    4. Terashima M.,
    5. Lamers M. C.,
    6. Köhler G. and
    7. Reth M.
    (1994). Two new proteins preferentially associated with membrane immunoglobulin D. EMBO J 13, 3793–3800
    OpenUrlPubMedWeb of Science
    1. Kornfeld S.
    (1992). Structure and function of the mannose 6-phosphate/ insulinlike growth factor II receptors. Annu. Rev. Biochem 61, 307–330
    OpenUrlCrossRefPubMedWeb of Science
    1. Lasky L. A.,
    2. Singer M. S.,
    3. Dowbenko D.,
    4. Imai Y.,
    5. Henzel W. J.,
    6. Grimley C.,
    7. Fennie C.,
    8. Gillett N.,
    9. Watson S. R. and
    10. Rosen S. D.
    (1992). An endothelial ligand for L-selectin is a novel mucin-like molecule. Cell 69, 927–938
    OpenUrlCrossRefPubMedWeb of Science
    1. Lasky L. A.
    (1995). Selectin-carbohydrate interactions and the initiation of the inflammatory response. Annu. Rev. Biochem 64, 113–139
    OpenUrlCrossRefPubMedWeb of Science
    1. Lenter M.,
    2. Levinovitz A.,
    3. Isenmann S. and
    4. Vestweber D.
    (1994). Monospecific and common glycoprotein ligands for E-and P-selectin on myeloid cells. J. Cell Biol 125, 471–481
    OpenUrlAbstract/FREE Full Text
    1. Levinovitz A.,
    2. Muhlhoff J.,
    3. Isenmann S. and
    4. Vestweber D.
    (1993). Identification of a glycoprotein ligand for E-selectin on mouse myeloid cells. J. Cell Biol 121, 449–459
    OpenUrlAbstract/FREE Full Text
    1. Linstedt A. D. and
    2. Hauri H.-P.
    (1993). Giantin, a novel conserved Golgi membrane protein containing a cytoplasmic domain of at least 350 kDa. Mol. Biol. Cell 4, 679–693
    OpenUrlAbstract/FREE Full Text
    1. Moore K. L.,
    2. Patel K. D.,
    3. Bruehl R. E.,
    4. Fugang L.,
    5. Johnson D. A.,
    6. Lichenstein H. S.,
    7. Cummings R. D.,
    8. Bainton D. F. and
    9. McEver R. P.
    (1995). P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin. J. Cell Biol 128, 661–671
    OpenUrlAbstract/FREE Full Text
    1. Piala L.,
    2. Albelda S. M.,
    3. Baldwin H. S.,
    4. Hammel P.,
    5. Gisler R. H. and
    6. Imhof B. A.
    (1993). Murine platelet endothelial cell adhesion molecule (PECAM-1)/CD31 modulates2 integrins on lymphokine-activated killer cells. Eur. J. Immunol 23, 2464–2471
    OpenUrlCrossRefPubMedWeb of Science
    1. Piatigorsky J. P.,
    2. O'Brien W. E.,
    3. Norman B. L.,
    4. Kalumuck K.,
    5. Wistow G. J.,
    6. Borras T.,
    7. Nickerson J. M. and
    8. Wawrousek E. F.
    (1988). Gene sharing by d-crystallin and argininosuccinate lysate. Proc. Nat. Acad. Sci. USA 85, 3479–3483
    OpenUrlAbstract/FREE Full Text
    1. Piatigorsky J. and
    2. Wistow G. J.
    (1989). Enzyme/crystallins: gene sharing as an evolutionary strategy. Cell 57, 197–199
    OpenUrlCrossRefPubMedWeb of Science
    1. Picker L. J.,
    2. Warnock R. A.,
    3. Burns A. R.,
    4. Doerschuk C. M.,
    5. Berg E. L. and
    6. Butcher E. C.
    (1991). The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140. Cell 66, 921–933
    OpenUrlCrossRefPubMedWeb of Science
    1. Pouyani T. and
    2. Seed B.
    (1995). PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus. Cell 83, 333–343
    OpenUrlCrossRefPubMedWeb of Science
    1. Sako D.,
    2. Chang X. J.,
    3. Barone K. M.,
    4. Vachino G.,
    5. White H. M.,
    6. Shaw G.,
    7. Veldman G. M.,
    8. Bean K. M.,
    9. Ahern T. J.,
    10. Furie B.,
    11. Cumming D. A. and
    12. Larsen G. R.
    (1993). Expression cloning of a functional glycoprotein ligand for P-selectin. Cell 75, 1179–1186
    OpenUrlCrossRefPubMedWeb of Science
    1. Sako D.,
    2. Comess K. M.,
    3. Barone K. M.,
    4. Camphausen R. T.,
    5. Cumming D. A. and
    6. Shaw G. D.
    (1995). A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding. Cell 83, 323–331
    OpenUrlCrossRefPubMedWeb of Science
    1. Springer T. A.
    (1994). Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm. Cell 76, 301–314
    OpenUrlCrossRefPubMedWeb of Science
    1. Steegmaier M.,
    2. Levinovitz A.,
    3. Isenmann S.,
    4. Borges E.,
    5. Lenter M.,
    6. Kocher H. P.,
    7. Kleuser B. and
    8. Vestweber D.
    (1995). The E-selectin-ligand ESL-1 is a variant of a receptor for fibroblast growth factor. Nature 373, 615–620
    OpenUrlCrossRefPubMedWeb of Science
    1. Stieber A.,
    2. Mourelatos Z.,
    3. Chen Y.-J.,
    4. Le Douarin N. and
    5. Gonatas N. K.
    (1995). MG160, a membrane protein of the Golgi apparatus which is homologous to a fibroblast growth factor receptor and to a ligand for E-selectin, is found only in the Golgi apparatus and appears early in chicken embryo development. Exp. Cell Res 219, 562–570
    OpenUrlCrossRefPubMedWeb of Science
    1. Tartaglia L. A. and
    2. Goeddel D. V.
    (1992). Two TNF receptors. Immunol. Today 13, 151–153
    OpenUrlCrossRefPubMedWeb of Science
    1. Vestweber D.
    (1994). Glycoprotein ligands of the two endothelial selectins. Res. Immunol 144, 704–708
    OpenUrl
    1. von Andrian U. H.,
    2. Hasslen S. R.,
    3. Nelson R. D.,
    4. Erlandsen S. L. and
    5. Butcher E. C.
    (1995). A central role for microvillous receptor presentation in leukocyte adhesion under flow. Cell 82, 989–999
    OpenUrlCrossRefPubMedWeb of Science
    1. Weller A.,
    2. Isenmann S. and
    3. Vestweber D.
    (1992). Cloning of the mouse endothelial selectins: Expression of both E and P-selectin is inducible by tumor necrosis factor-. J. Biol. Chem 267, 15176–15183
    OpenUrlAbstract/FREE Full Text
    1. Wistow G. J.,
    2. Mulders J. W. M. and
    3. de Jong W. W.
    (1987). The enzyme lactate dehyrogenase as a structural protein in avian and crocodilian lenses. Nature 326, 622–624
    OpenUrlCrossRefPubMed
    1. Wistow G. J.,
    2. Lietman T.,
    3. Williams L. A.,
    4. Stapel S. O.,
    5. De Jong W. W.,
    6. Horwitz J. and
    7. Piatigorsky J.
    (1988). t-Crystallin/-enolase: one gene encodes both an enzyme and a lens structural protein. J. Cell Biol 107, 2729–2736
    OpenUrlAbstract/FREE Full Text
    1. Zhou Z. and
    2. Olwin B. B.
    (1994). Characterization of the FGF binding domain of the cysteine-rich FGF receptor. Mol. Biol. Cell 5, 136–.
    OpenUrl
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Journal Articles
The E-selectin-ligand ESL-1 is located in the Golgi as well as on microvilli on the cell surface
M. Steegmaier, E. Borges, J. Berger, H. Schwarz, D. Vestweber
Journal of Cell Science 1997 110: 687-694;
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