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T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber
D.C. Shutt, L.M. Jenkins, E.J. Carolan, J. Stapleton, K.J. Daniels, R.C. Kennedy, D.R. Soll
Journal of Cell Science 1998 111: 99-109;
D.C. Shutt
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L.M. Jenkins
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E.J. Carolan
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J. Stapleton
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K.J. Daniels
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R.C. Kennedy
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D.R. Soll
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Summary

A chemotaxis chamber has been developed to analyze both the velocity and the directionality of individual T cells in gradients of high molecular mass molecules over long periods of time. Employing this chamber, it is demonstrated that syncytia induced by HIV in SUP-T1 cell cultures release two T cell chemoattractants with approximate molecular masses of 30 and 120 kDa. Neither uninfected single cells nor polyethylene glycol-induced syncytia release detectable chemoattractant, suggesting that these chemoattractants are linked to HIV infection. Soluble gp120 functions as a T cell chemoattractant and the addition of anti-gp120 antibody to syncytium-conditioned medium blocks the high molecular mass chemoattractant activity but not the low molecular mass activity. The addition of anti-CD4 antibody to syncytium-conditioned medium also blocks the high molecular mass chemoattractant activity but not the low molecular mass activity. These results demonstrate that HIV-induced T cell syncytia release a low and a high molecular mass T cell chemoattractant, and suggest that the high molecular mass factor is gp120 and that it functions through the CD4 receptor.

  • © 1998 by Company of Biologists

REFERENCES

    1. Attanasio R. J. S.,
    2. Anderson S. A.,
    3. Chanh T. C. and
    4. Kennedy R. C.
    (1991). Anti-idiotypic antibody response to monoclonal anti-CD4 preparations in nonhuman primate species. J. Immunol 146, 507–514
    OpenUrlAbstract
    1. Boyden S. V.
    (1962). The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J. Exp. Med 115, 453–466
    OpenUrlAbstract
    1. Center D. M. and
    2. Cruikshank W. W.
    (1982). Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J. Immunol 128, 2563–2568
    OpenUrlPubMedWeb of Science
    1. Chen T. L. L.,
    2. Kowalczyk P. A.,
    3. Ho G. and
    4. Chrisholm R. L.
    (1995). Targeted disruption of the Dictyostelium myosin essential light chain produce cells defective in cytokinesis and morphogenesis. J. Cell Sci 108, 3207–3218
    OpenUrlAbstract/FREE Full Text
    1. Cruikshank W. W. and
    2. Center D. M.
    (1982). Modulation of lymphocyte migration by human lymphokines. II. Purification of a lymphocyte chemoattractant factor (LCF). J. Immunol 128, 2569–2574
    OpenUrlPubMedWeb of Science
    1. Cruikshank W. W.,
    2. Greenstein J. L.,
    3. Theodore A. C. and
    4. Center D. M.
    (1991). Lymphocyte chemoattractant factor induces CD4-dependent intracytoplasmic signaling in lymphocytes. J. Immunol 146, 2948–2954
    OpenUrl
    1. Gelderblom H. R.,
    2. Reupke H. and
    3. Pauli G.
    (1985). Loss of envelope antigens of HTLV-III/LAV: a factor in AIDS pathogenesis?. Lancet 2, 1016–1017
    OpenUrlPubMedWeb of Science
    1. Kowolski M.,
    2. Potz J.,
    3. Basiropour L.,
    4. Goh W. C.,
    5. Terwiliger E.,
    6. Dayton A.,
    7. Rosen C.,
    8. Haseltine W. and
    9. Sodroski J.
    (1987). Functional regions of the envelope glycoprotein of human immunodeficiency virus type 1. Science 237, 1351–1355
    OpenUrlAbstract/FREE Full Text
    1. Kornfeld H.,
    2. Cruikshank W. W.,
    3. Pyle S. W.,
    4. Berman J. S. and
    5. Center D. M.
    (1988). Lymphocyte activation by HIV-1 envelope glycoprotein. Nature 335, 445–448
    OpenUrlCrossRefPubMed
    1. Lackie J. M. and
    2. Burns M. D.
    (1983). Leukocyte locomotion: comparison of random and directed paths using a modified time-lapse film analysis. J. Immunol. Meth 62, 109–122
    OpenUrlCrossRefPubMed
    1. Larsen C. G.,
    2. Anderson A. O.,
    3. Appella E.,
    4. Oppenheim J. J. and
    5. Matsushima K.
    (1989). The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science 243, 1464–1466
    OpenUrlAbstract/FREE Full Text
    1. Lifson J.,
    2. Feinberg M.,
    3. Reyes G.,
    4. Rabin L.,
    5. Banapour B.,
    6. Chakrabarti S.,
    7. Moss B.,
    8. Wong-Staal F.,
    9. Steimar K. and
    10. Engleman E.
    (1986). Induction of CD4-dependent cell fusion by the HTLV-III/LAV envelope glycoprotein. Nature 323, 725–728
    OpenUrlCrossRefPubMed
    1. Lifson J. D.,
    2. Feinberg J. D.,
    3. Reyes G. R.,
    4. McGrath M. S.,
    5. Stein B. S. and
    6. Engleman E. G.
    (1986). AIDS retrovirus induced cytopathology: Giant cell formation and involvement of CD4 antigen. Science 232, 1123–1127
    OpenUrlAbstract/FREE Full Text
    1. McDougal J. S.,
    2. Kennedy M. S.,
    3. Sligh J. M.,
    4. Cort S. P.,
    5. Mawle A. and
    6. Nicholson J. K.
    (1986). Binding of HTLV-III/LAV to T4+ cells by a complex of the 110K viral protein and the T4 molecule. Science 231, 382–385
    OpenUrlAbstract/FREE Full Text
    1. Mitola S.,
    2. Sozzani S.,
    3. Luini W.,
    4. Primo L.,
    5. Borsatti A.,
    6. Weich H. and
    7. Bussolino F.
    (1997). Tat-Human Immunodeficiency Virus-1 induces monocyte chemotaxis by activation of vascular endothelial growth factor receptor-1. Blood 90, 1365–1372
    OpenUrlAbstract/FREE Full Text
    1. Murphy S.,
    2. Sylwester A.,
    3. Kennedy R. and
    4. Soll D. R.
    (1995). Phagocytosis of individual CD4+cells by HIV-induced T cell syncytia. AIDS Res. Hum. Retrovir 11, 433–442
    OpenUrlPubMed
    1. Murray J. H.,
    2. Vawter-Hugert H.,
    3. Voss E. and
    4. Soll D. R.
    (1992). A three-dimensional motility cycle in leukocytes. Cell Motil. Cytoskel 22, 211–223
    OpenUrlCrossRefPubMedWeb of Science
    1. O'Neill G. J. and
    2. Parrott D. M. V.
    (1977). Locomotion of human lymphoid cells. I. Effect of culture and conA on T and non-T lymphocytes. Cell. Immunol 33, 257–267
    OpenUrlCrossRefPubMedWeb of Science
    1. Rhodes J. M.
    (1982). Measurement of chemotaxis in boyden chamber filter assays. Is the checkerboard correction valid?. J. Immunol. Meth 49, 235–236
    OpenUrlCrossRefPubMed
    1. Røsok B.,
    2. Bostad L.,
    3. Voltersvik P.,
    4. Bjerknes R.,
    5. Olofsson J.,
    6. Åsjö B. and
    7. Brinchmann J. E.
    (1996). Reduced CD4 cell counts in blood do not reflect CD4 cell depletion in tonsillar tissue in asymptomatic HIV-1 infection. AIDS 10, 35–.
    OpenUrl
    1. Schall T. J.,
    2. Bacon K.,
    3. Toy K. J. and
    4. Goeddel D. V.
    (1990). Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature 347, 669–671
    OpenUrlCrossRefPubMed
    1. Schall T. J.
    (1991). Biology of the RANTES/SIS cytokine family. Cytokine 3, 165–183
    OpenUrlCrossRefPubMedWeb of Science
    1. Schneider J.,
    2. Kaaden O.,
    3. Copeland T. D.,
    4. Orozslan S. and
    5. Hunsmann G.
    (1986). Shedding and interspecies type seroreactivity of the envelope glycoprotein gp120 of the human immunodeficiency virus. J. Gen. Virol 67, 2533–2538
    OpenUrlAbstract/FREE Full Text
    1. Shutt D. C.,
    2. Stapleton J. T.,
    3. Kennedy R. C. and
    4. Soll D. R.
    (1995). HIV-induced syncytia in peripheral blood cell cultures crawl by extending giant pseudopods. Cell. Immunol 166, 261–274
    OpenUrlCrossRefPubMedWeb of Science
    1. Shutt D. C.,
    2. Wessels D.,
    3. Wagenknecht K.,
    4. Chandrasekhar A.,
    5. Hitt A. L.,
    6. Luna E. J. and
    7. Soll D. R.
    (1995). Ponticulin plays a role in the positional stabilization of pseudopods. J. Cell Biol 131, 1495–1506
    OpenUrlAbstract/FREE Full Text
    1. Smith S. D.,
    2. Shatsky M.,
    3. Cohen P. S.,
    4. Warake R.,
    5. Link M. P. and
    6. Glader B. E.
    (1984). Monoclonal antibody and enzymatic profiles of human malignant T lymphoid cells and derived lines. Cancer Res 44, 5657–5660
    OpenUrlAbstract/FREE Full Text
    1. Soll D. R.
    (1987). Methods for manipulating and investigating developmental timing in Dictyostelium discoideum. Meth. Cell Biol 28, 413–431
    OpenUrlPubMedWeb of Science
    1. Soll D. R. and
    2. Kennedy R. C.
    (1994). The role of T cell motility and cytoskeletal reorganization in HIV-induced syncytium formation: a perspectus. AIDS Res. Hum. Retrovir 10, 325–327
    OpenUrlPubMed
    1. Soll D. R.
    (1995). The use of computers in understanding how animal cells crawl. Int. Rev. Cytol 163, 43–104
    OpenUrlPubMedWeb of Science
    1. Soll D. R.
    (1997). Researchers in cell motility and the cytoskeleton can play major roles in understanding AIDS. Cell Motil. Cytoskel 37, 91–97
    OpenUrlCrossRefPubMedWeb of Science
    1. Sylwester A.,
    2. Wessels D.,
    3. Anderson S. A.,
    4. Warren R. Q.,
    5. Shutt D.,
    6. Kennedy R. and
    7. Soll D. R.
    (1993). HIV-induced syncytia of a T cell line form single giant pseudopods and are motile. J. Cell Sci 106, 941–953
    OpenUrlAbstract/FREE Full Text
    1. Sylwester A.,
    2. Shutt D.C.,
    3. Wessels D.,
    4. Stapleton J. T.,
    5. Stites J.,
    6. Kennedy R. C. and
    7. Soll D. R.
    (1995). T cells and HIV-induced T cell syncytia exhibit the same motility cycle. J. Leuk. Biol 57, 643–650
    OpenUrlAbstract
    1. Sylwester A.,
    2. Murphy S.,
    3. Shutt D. and
    4. Soll D. R.
    (1997). HIV-induced T cell syncytia are self-perpetuating and the primary cause of T cell death in culture. J. Immunol 158, 3996–4007
    OpenUrlAbstract
    1. Varnum-Finney B.,
    2. Voss E. and
    3. Soll D. R.
    (1987). Frequency and orientation of pseudopod formation of Dicytosteliumdiscoideum amebae chemotaxing in a spatial gradient: Further evidence for a temporal mechanism. Cell Motil. Cytoskel 8, 18–26
    OpenUrlCrossRefPubMedWeb of Science
    1. Vaughan V. L.,
    2. Hansen D. and
    3. Stadler J.
    (1976). Parameters of polyethylene glycol-induced cell fusion and hybridization in lymphoid cell lines. Somat. Cell Genet 2, 537–544
    OpenUrl
    1. Wessels D.,
    2. Soll D. R.,
    3. Knecht D. A.,
    4. Loomis W. F.,
    5. DeLozanne A. and
    6. Spudich J.
    (1988). Cell motility and chemotaxis in Dictyostelium amoebae lacking myosin heavy chain. Dev. Biol 128, 164–177
    OpenUrlCrossRefPubMedWeb of Science
    1. Wessels D.,
    2. Schroeder N. A.,
    3. Voss E.,
    4. Hall A. L.,
    5. Condeelis J. and
    6. Soll D. R.
    (1989). cAMP-mediated inhibition of intracellular particle movement and actin reorganization in Dictyostelium. J. Cell Biol 109, 2841–2851
    OpenUrlAbstract/FREE Full Text
    1. Zigmond S. H. and
    2. Hirsch J. G.
    (1973). Leukocyte locomotion and chemotaxis. J. Exp. Med 137, 387–410
    OpenUrlAbstract
    1. Zigmond S. H.
    (1977). The ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J. Cell Biol 75, 606–616
    OpenUrlAbstract/FREE Full Text
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Journal Articles
T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber
D.C. Shutt, L.M. Jenkins, E.J. Carolan, J. Stapleton, K.J. Daniels, R.C. Kennedy, D.R. Soll
Journal of Cell Science 1998 111: 99-109;
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T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber
D.C. Shutt, L.M. Jenkins, E.J. Carolan, J. Stapleton, K.J. Daniels, R.C. Kennedy, D.R. Soll
Journal of Cell Science 1998 111: 99-109;

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