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Journal Articles
The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers
A. Huyett, J. Kahana, P. Silver, X. Zeng, W.S. Saunders
Journal of Cell Science 1998 111: 295-301;
A. Huyett
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J. Kahana
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P. Silver
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X. Zeng
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W.S. Saunders
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Summary

Microtubules provide the substrate for intracellular trafficking by association with molecular motors of the kinesin and dynein superfamilies. Motor proteins are generally thought to function as force generating units for transport of various cargoes along the microtubule polymer. Recent work suggests additional roles for motor proteins in changing the structure of the microtubule network itself. We report here that in the budding yeast Saccharomyces cerevisiae microtubule motors have antagonistic effects on microtubule numbers and lengths. As shown previously, loss of the Kar3p motor stimulates cytoplasmic microtubule growth while loss of Kip2p leads to a sharp reduction in cytoplasmic microtubule numbers. Loss of both the Kip2p and Kar3p motors together in the same cell produces an intermediate phenotype, suggesting that these two motors act in opposition to control cytoplasmic microtubule density. A Kip2p-GFP fusion from single gene expression is most concentrated at the spindle poles, as shown previously for an epitope tagged Kar3p-HA, suggesting both of these motors act from the minus ends of the microtubules to influence microtubule numbers.

  • © 1998 by Company of Biologists

REFERENCES

    1. Bascom-Slack C. A. and
    2. Dawson D. S.
    (1997). The Yeast Motor Protein, Kar3p, Is Essential for Meiosis I. J. Cell Biol 139, 459–67
    OpenUrlAbstract/FREE Full Text
    1. Carminati J. L. and
    2. Stearns T.
    (1997). Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex. J. Cell Biol 138, 629–641
    OpenUrlAbstract/FREE Full Text
    1. Cottingham F. R. and
    2. Hoyt M. A.
    (1997). Mitotic spindle positioning in Saccharomyces cerevisiae is accomplished by antagonistically acting microtubule motor proteins. J. Cell Biol 138, 1041–1053
    OpenUrlAbstract/FREE Full Text
    1. DeZwaan T. M.,
    2. Ellingson E.,
    3. Pellman D. and
    4. Roof D. M.
    (1997). Kinesin-related KIP3 of Saccharomyces cerevisiae is required for a distinct step in nuclear migration. J. Cell Biol 138, 1023–40
    OpenUrlAbstract/FREE Full Text
    1. Endow S.,
    2. Kang S.,
    3. Satterwhite L.,
    4. Rose M.,
    5. Skeen V. and
    6. Salmon E.
    (1994). Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBOJ 13, 2708–2713
    OpenUrlPubMedWeb of Science
    1. Farkasovsky M. and
    2. Kuntzel H.
    (1995). Yeast Num1p associates with the mother cell cortex during S/G2phase and affects microtubular functions. J. Cell Biol 131, 1003–1014
    OpenUrlAbstract/FREE Full Text
    1. Hoyt M. A.,
    2. He L.,
    3. Loo K. K. and
    4. Saunders W. S.
    (1992). Two Saccharomyces cerevisiae kinesin-related gene-products required for mitotic spindle assembly. J. Cell Biol 118, 109–120
    OpenUrlAbstract/FREE Full Text
    1. McMillan J. N. and
    2. Tatchell K.
    (1994). The JNM1 gene in the yeast Saccharomyces cerevisiae is required for nuclear migration and spindle orientation during the mitotic cell cycle. J. Cell Biol 125, 143–158
    OpenUrlAbstract/FREE Full Text
    1. Meluh P. B. and
    2. Rose M. D.
    (1990). Antibodies to the kinesin motor domain and CENP-E inhibit microtubule depolymerization-dependent motion of chromosomes in vitro. Cell 60, 1029–1041
    OpenUrlCrossRefPubMedWeb of Science
    1. Nicklas R. B.
    (1998). The forces that move chromosomes in mitosis. Annu. Rev. Biophys. Chem 17, 431–449
    OpenUrl
    1. Olmstead J. B.
    (1986). Microtubule-associated proteins. Ann. Rev. Cell Biol 2, 421–457
    OpenUrlCrossRefWeb of Science
    1. Page B. D.,
    2. Satterwhite L. L.,
    3. Rose M. D. and
    4. Snyder M.
    (1993). Localization of the KAR3 kinesin heavy chain-like protein requires the CIK1 interacting protein. J. Cell Biol 124, 507–519
    OpenUrlCrossRef
    1. Roof D. M.,
    2. Meluh P. B. and
    3. Rose M. D.
    (1991). Multiple kinesin-related proteins in yeast mitosis. Cold Spring Harbor Symp. Quant. Biol 56, 693–703
    OpenUrlAbstract/FREE Full Text
    1. Roof D. M.,
    2. Meluh P. B. and
    3. Rose M. D.
    (1992). Kinesin-related proteins required for assembly of the mitotic spindle. J. Cell Biol 118, 95–108
    OpenUrlAbstract/FREE Full Text
    1. Rout M. P. and
    2. Kilmartin J. V.
    (1990). Components of the yeast spindle and spindle pole body. J. Cell Biol 111, 1913–1927
    OpenUrlAbstract/FREE Full Text
    1. Saunders W.,
    2. Hornack D.,
    3. Lengyel V. and
    4. Deng C.
    (1997). The Saccharomyces cerevisiae kinesin-related motor Kar3p acts at preanaphase spindle poles to limit the number and length of cytoplasmic microtubules. J. Cell Biol 137, 417–431
    OpenUrlAbstract/FREE Full Text
    1. Saunders W.,
    2. Lengyel V. and
    3. Hoyt M. A.
    (1997). Mitotic spindle function in Saccharomyces cerevisiae requires a balance between different types of kinesin-related motors. Mol. Biol. Cell 8, 1025–1033
    OpenUrlAbstract/FREE Full Text
    1. Saunders W. S. and
    2. Hoyt M. A.
    (1992). Kinesin-related proteins required for structural integrity of the mitotic spindle. Cell 70, 451–458
    OpenUrlCrossRefPubMedWeb of Science
    1. Shaw S. L.,
    2. Yeh F.,
    3. Maddox P.,
    4. Salmon E. D. and
    5. Bloom K.
    (1998). Astral microtutule dynamics in yeast: A microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud. J. Cell Biol 139, 985–994
    OpenUrl
    1. Winey M.,
    2. Mamay C. L.,
    3. O'Toole E. T.,
    4. Mastronarde D. N. T. H.,
    5. Giddings J.,
    6. McDonald K. L. and
    7. McIntosh J. R.
    (1995). Three-dimensional ultrastructural analysis of the Saccharomyces cerevisiae mitotic spindle. J. Cell Biol 129, 1601–1616
    OpenUrlAbstract/FREE Full Text
    1. Yeh E.,
    2. Skibbens R.,
    3. Cheng J.,
    4. Salmon E. and
    5. Bloom K.
    (1995). Spindle dynamics and cell cycle regulation of dynein in the budding yeast Saccharomyces cerevisiae. J. Cell Biol 130, 687–700
    OpenUrlAbstract/FREE Full Text
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Journal Articles
The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers
A. Huyett, J. Kahana, P. Silver, X. Zeng, W.S. Saunders
Journal of Cell Science 1998 111: 295-301;
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The Kar3p and Kip2p motors function antagonistically at the spindle poles to influence cytoplasmic microtubule numbers
A. Huyett, J. Kahana, P. Silver, X. Zeng, W.S. Saunders
Journal of Cell Science 1998 111: 295-301;

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