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Journal Article
Fast transport of neurofilament protein along microtubules in squid axoplasm
V. Prahlad, B.T. Helfand, G.M. Langford, R.D. Vale, R.D. Goldman
Journal of Cell Science 2000 113: 3939-3946;
V. Prahlad
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B.T. Helfand
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G.M. Langford
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R.D. Vale
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R.D. Goldman
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Summary

Using squid axoplasm as a model system, we have visualized the fast transport of non-filamentous neurofilament protein particles along axonal microtubules. This transport occurs at speeds of 0.5-1.0 microm/second and the majority of neurofilament particles stain with kinesin antibody. These observations demonstrate, for the first time, that fast (0.5-1.0 microm/second) transport of neurofilament proteins occurs along microtubules. In addition, our studies suggest that neurofilament protein can be transported as non-membrane bound, nonfilamentous subunits along axons, and that the transport is kinesin-dependent. Microtubule-based fast transport might therefore provide a mechanism for the distribution and turnover of neurofilament, and perhaps other cytoskeletal proteins, throughout neurons.

  • © 2000 by Company of Biologists

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Journal Article
Fast transport of neurofilament protein along microtubules in squid axoplasm
V. Prahlad, B.T. Helfand, G.M. Langford, R.D. Vale, R.D. Goldman
Journal of Cell Science 2000 113: 3939-3946;
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Journal Article
Fast transport of neurofilament protein along microtubules in squid axoplasm
V. Prahlad, B.T. Helfand, G.M. Langford, R.D. Vale, R.D. Goldman
Journal of Cell Science 2000 113: 3939-3946;

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