cAMP receptor affinity controls wave dynamics, geometry and morphogenesis in Dictyostelium

Dirk Dormann1^1, Ji-Yun Kim2^2, Peter N. Devreotes², and Cornelis J. Weijer¹

¹School of Life Sciences, Division of Cell and Developmental Biology, University of Dundee, Wellcome Trust Biocentre, Dow Street, Dundee, DD1 5EH, UK
²Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA

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Movie 1

Comparison of the darkfield wave patterns during early aggregation (see Fig. 1). Dark field wave patterns during early aggregation in the parental strain DH1 and the mutant cell lines cAR1/RI9, cAR3/RI9 and N272/RI9. Time interval between images: 20 seconds.

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Movie 2

Optical density waves in mounds (see Fig. 4). Wave propagation at the mound stage in the parental strain DH1 and the mutant cell lines cAR1/RI9, cAR3/RI9 and N272/RI9. Time interval between images: 10 seconds.

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Multiarmed spirals in the cAR2 and cAR3 null strains (see Fig. 5). Multi-armed spiral waves in mounds of the cAR2 null and the cAR3 null cell lines. Time interval between images: 10 seconds.

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Movie 4

Wave initiation in a cAR1 affinity receptor mutants (see Fig. 9). Randomly appearing concentric dark field waves in the cAR1 receptor mutant IIIb21h. The waves disappear at the end of the sequence. Time interval between images: 20 seconds.