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Research Article
Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3-expressing 293T and melanoma cells by CrkII and Rho-mediated signalling
Isobel D. Lawrenson, Sabine H. Wimmer-Kleikamp, Peter Lock, Simone M. Schoenwaelder, Michelle Down, Andrew W. Boyd, Paul F. Alewood, Martin Lackmann
Journal of Cell Science 2002 115: 1059-1072;
Isobel D. Lawrenson
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Sabine H. Wimmer-Kleikamp
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Peter Lock
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Simone M. Schoenwaelder
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Michelle Down
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Andrew W. Boyd
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Paul F. Alewood
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Martin Lackmann
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  • Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3-expressing 293T and melanoma cells by CrkII and Rho-mediated signalling

    Isobel D. Lawrenson1, Sabine H. Kleikamp1, Peter Lock1, Simone M. Schoenwaelder2, Michelle Down3, Andrew W. Boyd3, Paul F. Alewood4 and Martin Lackmann1




    MPEG MovieJPEG Image

    Movie 1

    Ephrin-A5 induced rounding and de-adhesion of AO9 melanoma cells. AO9 melanoma cells, plated to semi-confluency onto fibronectin-coated microscopic glass coverslips, were serum-starved and then stimulated by addition of clustered ephrin-A5 Fc to 10 nM final concentration. Microscopic images of a representative section of a slide were taken every minute during a time course 10 minutes before and 50 minutes after ephrin addition (indicated by a blacked-out frame at the beginning of the video) and processed using the Confocal Assistant Software (ver. 2.55, Bio-Rad) (see Fig. 2).


    MPEG MovieJPEG Image

    Movie 2

    Clustered ephrin-A5 triggers rapid cell rounding and dynamic membrane blebbing of EphA3-293T cells. Serum-starved EphA3-293T cells on fibronectin-coated microscopic glass coverslips were treated with clustered ephrin-A5 Fc to induce cell rounding and dynamic membrane blebbing. The video represents microscopic images taken every minute, 10 minutes before and 50 minutes after ephrin-A5 Fc stimulation, which were processed as described for Movie 1 (see Fig. 9F).





    MPEG MovieJPEG Image

    Movie 3

    Ephrin-A5-induced membrane blebbing of EphA3-293T cells is abrogated by the ROCK inhibitor Y-27632. Parallel cultures of serum-starved EphA3-293T cells on microscopic glass coverslips were treated, prior to stimulation with clustered ephrin-A5 Fc, for 2 hours with with 10 mM Rock inhibitor Y-27632. Although the cells still round-up upon ephrin-A5 addition, membrane blebbing is completely abrogated (see Fig. 9F)..





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Research Article
Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3-expressing 293T and melanoma cells by CrkII and Rho-mediated signalling
Isobel D. Lawrenson, Sabine H. Wimmer-Kleikamp, Peter Lock, Simone M. Schoenwaelder, Michelle Down, Andrew W. Boyd, Paul F. Alewood, Martin Lackmann
Journal of Cell Science 2002 115: 1059-1072;
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Research Article
Ephrin-A5 induces rounding, blebbing and de-adhesion of EphA3-expressing 293T and melanoma cells by CrkII and Rho-mediated signalling
Isobel D. Lawrenson, Sabine H. Wimmer-Kleikamp, Peter Lock, Simone M. Schoenwaelder, Michelle Down, Andrew W. Boyd, Paul F. Alewood, Martin Lackmann
Journal of Cell Science 2002 115: 1059-1072;

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