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Research Article
Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface
Emilia Mira, Rosa Ana Lacalle, José María Buesa, Gonzalo González de Buitrago, Sonia Jiménez-Baranda, Concepción Gómez-Moutón, Carlos Martínez-A, Santos Mañes
Journal of Cell Science 2004 117: 1847-1857; doi: 10.1242/jcs.01035
Emilia Mira
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Rosa Ana Lacalle
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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José María Buesa
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Gonzalo González de Buitrago
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Sonia Jiménez-Baranda
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Concepción Gómez-Moutón
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Carlos Martínez-A
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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Santos Mañes
Department of Immunology and Oncology, Centro Nacional de Biotecnología/CSIC, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain
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  • For correspondence: smanes@cnb.uam.es
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Data supplements

  • JCS01035 Supplemental Figures

    Files in this Data Supplement:

    • Figure S1 -

      Fig. S1. Partitioning of MMP9 chimeras into distinct membrane domains. Confocal microscopy of MMP9-expressing cells stained with cholera toxin b-subunit (green) and anti-MMP9 antibody (red); yellow staining indicates colocalization of the molecules. (n =50). Bar, 10 mm.

    • Figure S2 -

      Fig. S2.Doxycycline does not affect the growth of mock xenografts. (A) Average tumor size at 17 weeks of xenografts implanted in untreated (-dox) and doxycycline-treated mice (+dox). (B) Tumor growth kinetics in mice represented in A over a 17-week period. Mean values � s.e.m. are represented.

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Keywords

  • Metalloprotease
  • Tumor growth
  • Angiogenesis
  • Lipid rafts
  • Membrane targeting

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Research Article
Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface
Emilia Mira, Rosa Ana Lacalle, José María Buesa, Gonzalo González de Buitrago, Sonia Jiménez-Baranda, Concepción Gómez-Moutón, Carlos Martínez-A, Santos Mañes
Journal of Cell Science 2004 117: 1847-1857; doi: 10.1242/jcs.01035
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Research Article
Secreted MMP9 promotes angiogenesis more efficiently than constitutive active MMP9 bound to the tumor cell surface
Emilia Mira, Rosa Ana Lacalle, José María Buesa, Gonzalo González de Buitrago, Sonia Jiménez-Baranda, Concepción Gómez-Moutón, Carlos Martínez-A, Santos Mañes
Journal of Cell Science 2004 117: 1847-1857; doi: 10.1242/jcs.01035

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