Data supplements
JCS01611 Supplemental Material
Files in this Data Supplement:
- Movie 1
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Movie 1. Live-cell imaging of eGFP-Huntingtin exon1[Q138] dynamics. Time course images of mutant polyglutamine-expanded huntingtin exon 1 fragment aggregate dynamics at 2 frames per second over 30 seconds. 200 msecond exposure per frame taken at 37ºC.
- Movie 2
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Movie 2. Live-cell imaging of eGFP-ataxin-1[Q84] dynamics. Time course images of mutant polyglutamine expanded ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds. 200 msecond exposure per frame taken at 37ºC.
- Movie 3
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Movie 3. Live-cell imaging of eGFP-ataxin-1[Q2] dynamics. Time course images of polyglutamine-removed human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.
- Movie 4
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Movie 4. Live-cell imaging of eGFP-ataxin-1[Q26] dynamics. Time course images of wild-type human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.
- Movie 5
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Movie 5. Live-cell imaging of eGFP-ataxin-1[Q84] dynamics. Time course images of polyglutamine-expanded mutant human ataxin-1 nuclear inclusion dynamics at 2 frames per second over 30 seconds in human HeLa cells. 200 msecond exposure per frame taken at 37ºC.
- Movie 6
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Movie 6. Live-cell imaging time course of a-amanatin treatment of eGFP-ataxin-1[Q84]-expressing cells. 25 mg/ml a-amanatin treatment time course over the last 2 hours of a 3 hour treatment at 37ºC showing dispersion of ataxin-1 nuclear inclusions. Time from addition of a-amanatin is indicated in lower left corner.
- Movie 1
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