JCS03152 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 - Adobe PDF
• Supplemental Figure 2 - Adobe PDF
• Supplemental Figure 3 - Adobe PDF
• Supplemental Figure 4 - Adobe PDF
• Movie 1 -

  Movie 1. (QuickTime movie accompanying Fig. 1A-C) Fluorescent images of a GFP-ABD-expressing vegetative wild-type cell showing blebbing in SB. Images were captured every 500 milliseconds.

• Movie 2 -

  Movie 2. (QuickTime movie accompanying Fig. 1E) Fluorescent images of a GFP-ABD-expressing vegetative Myosin-II-null cell extending filopodia in SB. Images were captured every 500 milliseconds.

• Movie 3 -

  Movie 3. (QuickTime movie accompanying Fig. 3) Fluorescent images of a GFP-ABD-expressing vegetative wild-type cell showing blebbing in SB, under a silicone-oil layer. Images were captured every 250 milliseconds and low-pass filtered at 1 Hz. The blue curve shows the smoothed cell boundary interpreted in the 2D map analysis.

• Movie 4 -

  Movie 4. (QuickTime movie accompanying Fig. 4A,C,E) Fluorescent images of a GFP-ABD-expressing wild-type cell showing chemotaxis in blebbing-mode (in SB). Images were captured every 500 milliseconds.

• Movie 5 -

  Movie 5. (QuickTime movie accompanying Fig. 4B,D,F) Fluorescent images of a GFP-ABD-expressing wild-type cell showing chemotaxis in filopodia-mode (in HI). Images were captured every 500 milliseconds.