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Short Report
Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site
Pier Paolo D'Avino, Tetsuya Takeda, Luisa Capalbo, Wei Zhang, Kathryn S. Lilley, Ernest D. Laue, David M. Glover
Journal of Cell Science 2008 121: 1151-1158; doi: 10.1242/jcs.026716
Pier Paolo D'Avino
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Tetsuya Takeda
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Luisa Capalbo
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Wei Zhang
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Kathryn S. Lilley
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Ernest D. Laue
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David M. Glover
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Summary

Anillin, one of the first factors recruited to the cleavage site during cytokinesis, interacts with actin, myosin II and septins, and is essential for proper organization of the actomyosin contractile ring. We employed affinity-purification methodology coupled with mass spectrometry to identify Anillin-interacting molecules in Drosophila cells. We isolated several actin and myosin proteins, three of the five Drosophila septins and RacGAP50C (Tum), a component of the centralspindlin complex. Using drug and RNA interference (RNAi) treatments we established that F-actin is essential for Anillin cortical localization in prometaphase but not for its accumulation at the cleavage furrow after anaphase onset. Moreover, septins were not recruited to the cleavage site in cells in which Anillin was knocked down by RNAi, but localized to central-spindle microtubules, suggesting that septins travel along microtubules to interact with Anillin at the furrow. Finally, we demonstrate that RacGAP50C is necessary for Anillin accumulation at the furrow and that the two proteins colocalize in vivo and interact in vitro. Thus, in addition to its role in activating RhoA signalling, RacGAP50C also controls the proper assembly of the actomyosin ring by interacting with Anillin at the cleavage furrow.

  • Anillin
  • Centralspindlin
  • Affinity purification
  • Protein complex
  • Accepted January 24, 2008.
  • © The Company of Biologists Limited 2008
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Short Report
Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site
Pier Paolo D'Avino, Tetsuya Takeda, Luisa Capalbo, Wei Zhang, Kathryn S. Lilley, Ernest D. Laue, David M. Glover
Journal of Cell Science 2008 121: 1151-1158; doi: 10.1242/jcs.026716
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Short Report
Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site
Pier Paolo D'Avino, Tetsuya Takeda, Luisa Capalbo, Wei Zhang, Kathryn S. Lilley, Ernest D. Laue, David M. Glover
Journal of Cell Science 2008 121: 1151-1158; doi: 10.1242/jcs.026716

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