JCS080762 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Early ER stress induced by thapsigargin leads to increased ER-mitochondrial coupling. (A,B) Quantification of the radial fluorescence of control HeLa cells, or cells treated with 1 µM thapsigargin for 4 hours. (C,D) Quantification of the Manders� coefficient M1 (fraction of ER in colocalization with mitochondria), or M2 (fraction of mitochondria in colocalization with ER), from control cells or cells treated with thapsigargin 1 µM for 4 hours. (E) Quantification of ATP levels after 4 h treatment with 1 µM thapsigargin. (F) Oxygen consumption rates were quantified from control cells, or cells treated with 1 µM thapsigargin for 4 hours. Data are expressed as mean±s.e.m. *P<0.05 vs. respective controls.

• Supplemental Figure S2 -

  Fig. S2. Mitochondrial rearrangement does not depend on the actin cytoskeleton. (A) Analysis of the radial fluorescence of Mitotracker-stained control HeLa cells, or cells treated for 4 hours with 0.5 µg/ml tunicamycin in combination with 10 µM cytochalasin D as indicated. (B) Analysis of the radial fluorescence of transiently expressing ER-targeted RFP HeLa cells untreated, or cells treated tunicamycin in combination with cytochalasin D as indicated. (C) Quantification of the Manders� coefficient M2 (fraction of mitochondria in colocalization with ER), of cells expressing ER-targeted RFP and stained with Mitotracker green. Data are expressed as mean±s.e.m. *P<0.05 vs. control cells within the same subcellular zone.