JCS0728790 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. Acid phosphatase staining in the RPE (arrows) from wild-type (A) and Nuc1 (B) rats at 1 year of age. Staining intensity is greater in the Nuc1 rat. Scale bar: 20 µm.

• Supplemental Figure S2 -

  Fig. S2. Western blots showing similar reactivities in wild-type and Nuc1 RPE samples for four proteins involved in the phagocytosis process. Actin was used as a loading control. The two heavy bands in the Rac1 blot are unidentified.

• Supplemental Figure S3 -

  Fig. S3. Analysis of the distribution of recombinant βA3-crystallin in the soluble (Sol) and insoluble (Insol) extracts. The left panel is the SDS-PAGE analysis of the protein fractions run on a 4−12% Bis-Tris gel and the right panel is the western blot analysis of the same samples run on a duplicate gel and probed with a peptide antibody specific for βA3/A1-crystallin.