Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Cell Scientists to Watch
    • First Person
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
    • For library administrators
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Cell Scientists to Watch
    • First Person
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contacts
    • Contact JCS
    • Subscriptions
    • Advertising
    • Feedback
    • For library administrators
Research Article
Various p53 mutant proteins differently regulate the Ras circuit to induce a cancer-related gene signature
Hilla Solomon, Yosef Buganim, Ira Kogan-Sakin, Leslie Pomeraniec, Yael Assia, Shalom Madar, Ido Goldstein, Ran Brosh, Eyal Kalo, Tsevi Beatus, Naomi Goldfinger, Varda Rotter
Journal of Cell Science 2012 125: 3144-3152; doi: 10.1242/jcs.099663
Hilla Solomon
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yosef Buganim
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ira Kogan-Sakin
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leslie Pomeraniec
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yael Assia
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shalom Madar
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ido Goldstein
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ran Brosh
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eyal Kalo
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tsevi Beatus
2Department of Materials and Interfaces, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Naomi Goldfinger
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Varda Rotter
1Department of Molecular Cell Biology, Weizmann Institute of Science, Rehovot 76100, Israel
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: varda.rotter@weizmann.ac.il
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF
Loading

Summary

Concomitant expression of mutant p53 and oncogenic Ras, leading to cellular transformation, is well documented. However, the mechanisms by which the various mutant p53 categories cooperate with Ras remain largely obscure. From this study we suggest that different mutant p53 categories cooperate with H-Ras in different ways to induce a unique expression pattern of a cancer-related gene signature (CGS). The DNA-contact p53 mutants (p53R248Q and p53R273H) exhibited the highest level of CGS expression by cooperating with NFκB. Furthermore, the Zn+2 region conformational p53 mutants (p53R175H and p53H179R) induced the CGS by elevating H-Ras activity. This elevation in H-Ras activity stemmed from a perturbed function of the p53 transcription target gene, BTG2. By contrast, the L3 loop region conformational mutant (p53G245S) did not affect CGS expression. Our findings were further corroborated in human tumor-derived cell lines expressing Ras and the aforementioned mutated p53 proteins. These data might assist in future tailor-made therapy targeting the mutant p53–Ras axis in cancer.

Footnotes

  • Funding

    This research was supported by a Center of Excellence grant from Flight Attendant Medical Research Institute, Yad Abraham Center for Cancer Diagnosis and Therapy [grant number 7034640901]; and the European Community EC FP7-INFLACARE [number 223151, grant number 7104370402]. This publication reflects the authors' views and not necessarily those of the European Community. The EC is not liable for any use that may be made of the information contained herein. V. R. is the incumbent of the Norman and Helen Asher Professorial Chair Cancer Research at the Weizmann Institute.

  • Supplementary material available online at http://jcs.biologists.org/lookup/suppl/doi:10.1242/jcs.099663/-/DC1

  • Accepted February 13, 2012.
  • © 2012. Published by The Company of Biologists Ltd
View Full Text
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Various p53 mutant proteins differently regulate the Ras circuit to induce a cancer-related gene signature
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Research Article
Various p53 mutant proteins differently regulate the Ras circuit to induce a cancer-related gene signature
Hilla Solomon, Yosef Buganim, Ira Kogan-Sakin, Leslie Pomeraniec, Yael Assia, Shalom Madar, Ido Goldstein, Ran Brosh, Eyal Kalo, Tsevi Beatus, Naomi Goldfinger, Varda Rotter
Journal of Cell Science 2012 125: 3144-3152; doi: 10.1242/jcs.099663
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Research Article
Various p53 mutant proteins differently regulate the Ras circuit to induce a cancer-related gene signature
Hilla Solomon, Yosef Buganim, Ira Kogan-Sakin, Leslie Pomeraniec, Yael Assia, Shalom Madar, Ido Goldstein, Ran Brosh, Eyal Kalo, Tsevi Beatus, Naomi Goldfinger, Varda Rotter
Journal of Cell Science 2012 125: 3144-3152; doi: 10.1242/jcs.099663

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
    • Summary
    • Introduction
    • Results
    • Discussion
    • Materials and Methods
    • Footnotes
    • References
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • An SNX10-dependent mechanism down-regulates fusion between mature osteoclasts
  • Combined heterogeneity in cell size and deformability promotes cancer invasiveness
  • Non-canonical ago loading of EV-derived exogenous single stranded miRNA in recipient cells
Show more RESEARCH ARTICLE

Similar articles

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

Follow us on Instagram

Cell science is bursting with beautiful images and over on Instagram, we’re showing them off! Find both JCS and FocalPlane on Instagram for stories and techniques across cell biology.


An interview with Derek Walsh

Professor Derek Walsh is the guest editor of our new special issue Cell Biology of Host-Pathogen Interactions. In an interview, Derek tells us about his work in the field of DNA viruses, the impact of the pandemic on virology and what his role as Guest Editor taught him.


How to improve your scientific writing

"If you are a scientist and you want to succeed, you must become a writer."

How do scientists become master storytellers? We called on our journal Editors, proofreaders and contributors to our community sites for their advice on how to improve your scientific writing.


Meet the preLighters: Jennifer Ann Black

Following the theme of our latest special issue, postdoc Jennifer Ann Black studies replication stress and genome plasticity in Leishmania in Professor Luiz Tosi’s lab in Sao Paolo. We caught up with Jenn (virtually) to hear about her relocation to Brazil mid-pandemic, her research on parasites and what she enjoys about ‘preLighting’.

In our special issue, Chandrakar et al. and Rosazza et al. present their latest work on Leishmania.


Mole – The Corona Files

“There are millions of people around the world who continue to believe that the Terrible Pandemic is a hoax.”

Mole continues to offer his wise words to researchers on how to manage during the COVID-19 pandemic.


JCS and COVID-19

For more information on measures Journal of Cell Science is taking to support the community during the COVID-19 pandemic, please see here.

If you have any questions or concerns, please do not hestiate to contact the Editorial Office.

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Fast-track manuscripts
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Manuscript transfer network
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contacts

  • Contact JCS
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2021   The Company of Biologists Ltd   Registered Charity 277992