ABSTRACT
Centrioles are evolutionarily conserved microtubule-based structures at the core of the animal centrosome that are essential for nucleating the axoneme of cilia. We hypothesized that centriole proteins have been under-represented in proteomic studies of the centrosome, because of the larger amount of pericentriolar material making up the centrosome. In this study, we have overcome this problem by determining the centriolar proteome of mammalian sperm cells, which have a pair of centrioles but little pericentriolar material. Mass spectrometry of sperm centrioles identifies known components of centrioles and many previously uncharacterized candidate centriole proteins. Assessment of localization of a subset of these candidates in cultured cells identified CCDC113, CCDC96, C4orf47, CCDC38, C7orf31, CCDC146, CCDC81 and CCDC116 as centrosome-associated proteins. We examined the highly conserved protein CCDC113 further and found that it is a component of centriolar satellites, is in a complex with the satellite proteins HAP1 and PCM1, and functions in primary cilium formation.
INTRODUCTION
The centriole is a microtubule-based structure that is conserved in all eukaryotes that make cilia (Hodges et al., 2010; Carvalho-Santos et al., 2011). The centriole has a unique ninefold symmetry in which compound microtubules (doublets and triplets) are radially arranged in a cylinder of ∼500 nm in length and ∼275 nm in diameter (Chrétien et al., 1997). Centrioles organize the centrosome by recruiting a matrix of associated pericentriolar material (PCM) that supports microtubule nucleation, polymerization and organization (Lüders and Stearns, 2007). Importantly, centrioles nucleate the axonemal microtubules of the cilium, thus imparting their unique structure on the axoneme (Fisch and Dupuis-Williams, 2011).
A combination of proteomic, bioinformatic and genomic approaches (Ostrowski et al., 2002; Avidor-Reiss et al., 2004; Li et al., 2004; Keller et al., 2005; Kilburn et al., 2007; McClintock et al., 2008; Keller et al., 2009; Jakobsen et al., 2011; Hoh et al., 2012; Ishikawa et al., 2012) has identified many centrosome and cilium proteins. The centriole proteomes of Chlamydomonas (Keller et al., 2005) and Tetrahymena (Kilburn et al., 2007) have been analyzed, but direct proteomic analysis of mammalian centrioles has not been reported. Morphological analysis of centrioles from several species (Li et al., 2012; Guichard et al., 2013) reveals conserved structural features, and, other than the ninefold symmetry of centrioles, which is dictated by the structure of SASS6 (Gönczy, 2012), these features have no known molecular basis. For example, cryotomography and 3D-reconstruction studies of Chlamydomonas (Li et al., 2012) and Trichonympha centrioles (Guichard et al., 2013) show non-tubulin densities associated with the lumen of microtubule doublets and triplets, and with the linker between them.
To identify centriole proteins, we exploited mammalian sperm cells, which have centrioles that have lost most associated PCM and the capacity to nucleate microtubules during spermatogenesis (Manandhar et al., 2005). A mature mammalian sperm typically has two orthogonal centrioles in the midpiece between head and tail, a well-defined proximal centriole and a degenerated distal centriole that nucleates the flagellar axoneme (Manandhar et al., 2000). Centrioles are paternally inherited in most vertebrates (Simerly et al., 1995), and once introduced into the egg by fertilization, the centrioles recruit PCM to form the centrosome (Stearns and Kirschner, 1994). Therefore, analysis of sperm centrioles might be informative in identifying components required for PCM organization and for cilium formation.
In this study, we isolated centrioles from bovine sperm and identified 364 proteins by mass spectrometry, of which we confirmed eight as previously uncharacterized centrosome-localizing proteins. One of these, CCDC113, is a component of centriolar satellites that is highly conserved and functions in cilium formation.
RESULTS AND DISCUSSION
We developed a protocol for enriching for sperm centrioles based on previous studies (Olson et al., 1976; Kleve and Clark, 1980). The centriole enrichment protocol was guided by the specialized structure of mature sperm cells, which consists of a head (acrosome and nucleus), a midpiece (proximal and distal centrioles, mitochondria) and a tail (axoneme) (Palermo et al., 1997). We chose bovine sperm as the starting material for the centriole enrichment; Fig. 1A shows these cells stained with antibodies against the centriole marker centrin, the axoneme marker tubulin and the DNA stain DAPI. Bovine sperm were sonicated to separate heads from tails, and sedimented through a glycerol cushion, isolating the tails in the supernatant (Fig. 1B). Successful fractionation was confirmed by immunostaining (Fig. 1C) and immunoblotting (Fig. 1D) for the nuclear marker histone H3 (heads fraction) and centrin (tail fraction). The tail fractions were sequentially extracted with buffers of increasing stringency to enrich for centrioles (Olson et al., 1976). Separation of centrioles from midpiece mitochondria and the bulk of the axoneme was confirmed by immunoblotting for the mitochondria marker MtHsp70, (first extraction fraction), centrin (second extraction fraction) and the axoneme marker tubulin (third extraction fraction) (Fig. 1E). Analysis of the centriole-enriched fractions (Fig. 1E, ext#2) from two independent experiments by liquid-chromatography mass spectrometry (LC-MS) yielded a candidate sperm centriole proteome of 364 proteins (supplementary material Table S1).
Centriole enrichment protocol from bovine sperm cells. (A) Bovine sperm, fixed and stained with antibodies against centrin and α-tubulin. DNA was stained with DAPI. Scale bar: 5 µm. (B) Intact sperm, isolated heads and tails imaged by phase-contrast microscopy. Scale bar: 10 µm. (C) Isolated tails fixed and stained for centrin and α-tubulin. Scale bar: 5 µm. (D) Extracts of intact sperm, isolated heads and tails blotted for centrioles, histone H3 and α-tubulin. (E) Extracts from each extraction step were blotted for centrin, tubulin and MtHsp70. (F) Categorization of sperm centriole candidate proteins according to the gene ontology molecular compartment. All insets are shown at 4× relative to the main image.
To assess the sperm centriole proteome candidates, all 364 proteins were categorized by gene ontology molecular compartment, and cross-referenced with proteomic, bioinformatic and genomic studies of centrosomes and cilia (Fig. 1F; supplementary material Table S2). A total of 60% of the sperm centriole candidate proteins had been previously identified in one or more of these studies, and 13 proteins had been previously shown to localize to the centrosome (supplementary material Table S2), including the centriole components centrin 1 (Hart et al., 1999) and POC1 (Keller et al., 2009). Few proteins were annotated as having a molecular compartment consistent with them being contaminants (Fig. 1F). In contrast, our analysis of the data from the previous studies of the mammalian sperm tail proteome (Baker et al., 2013), which did not enrich for the centriole fraction, revealed that only 11% of the identified proteins corresponded to previously identified centrosome and cilium proteins. Interestingly, 19% of the candidate proteins from our dataset did not have an associated molecular compartment, and most of these proteins are previously uncharacterized.
A subset of candidate proteins were selected for further characterization in cultured human cells, based on several criteria. CCDC96, CCDC113, CCDC81, LRRC34, LRRC48, WDR65 and C4orf47 were previously identified in one or more systematic studies of centrosomes and cilia; CCDC146, C4orf47, CCDC96 and CCDC113 are conserved in most ciliated eukaryotes; and EFCAB1, WDR65, LRRC34, LRRC48, TTC18, CCDC38, CCDC81, CCDC108 and CCDC116 contain domains or structural motifs found in proteins associated with centrosome or cilia. In addition to the above, C7orf31 and C9ORF116 were chosen randomly from among the uncharacterized proteins. Publically available expression data showed that these proteins varied in expression pattern, from wide distribution in most tissues to being highly-restricted to the testis (supplementary material Fig. S1B). The ability of these proteins to localize to the centrosome of somatic cells was assayed by cloning the corresponding cDNAs with N- and/or C-terminal GFP tags and expression by transient transfection in HeLa cells, which do not form a primary cilium, and RPE1 cells, which were induced to form a primary cilium by serum starvation (supplementary material Fig. S1B). CCDC146 localization was determined by immunostaining with antibody against CCDC146. Eight of the 15 tagged candidate proteins localized at or near the centrosome, as assessed by localization relative to γ-tubulin or polyglutamylated tubulin (Fig. 2). Most of the proteins largely colocalized with γ-tubulin; the exceptions were CCDC113, which localized to punctate structures resembling centriolar satellites, and CCDC146, which localized to only one centriole (Fig. 2), identified as the mother centriole by co-staining with CEP164 (supplementary material Fig. S1A). The remaining seven of the 15 tagged proteins did not localize to the centrosome (supplementary material Fig. S1B).
Localization of new centrosome proteins. (A) HeLa cells were transfected with the indicated constructs. 24 h after transfection, cells were fixed and stained with anti-GFP antibody or with anti-CCDC146 antibody. Centrosomes were stained with anti-γ-tubulin antibody and DNA was stained with DAPI. (B) RPE1 cells were transfected with the indicated constructs, serum-starved for 24 h, fixed and stained with anti-GFP antibody or with anti-CCDC146 antibody. The primary cilium and centrioles were stained for polyglutamylated tubulin (glut. tubulin) and DNA was stained with DAPI. Scale bar: 10 µm. All insets are shown at 4× relative to the main image.
We chose CCDC113 for further study, based on its putative localization to centriolar satellites. Centriolar satellites are linked to several centrosome-related processes including centrosome and cilium protein trafficking, ciliogenesis and centriole duplication (Firat-Karalar et al., 2014), and are implicated in ciliopathies (Bärenz et al., 2011). CCDC113 is related to CCDC96, one of the other centrosome-localizing proteins (supplementary material Fig. S2A), and orthologs of both proteins are present in chordates, deuterostomes, schistosomes, Trichoplax and Chlamydomonas, but not in C. elegans (supplementary material Fig. S2A). RT-PCR experiments using RNA from RPE1 cells (Fig. 3A) and immunoblotting with an antibody against CCDC113 (Fig. 3B) showed that CCDC113 is expressed in RPE1, U2OS and HeLa cells.
CCDC113 expression and localization in cultured human cell lines. (A) RT-PCR analysis of RNA from RPE1 cells with a pair of CCDC113-specific primers with (+) or without (−) the addition of reverse transcriptase. Positive control is PCR amplification of CCDC113 gene with these primers. (B) Extracts from RPE1, HeLa and U2OS cells were blotted for CCDC113 and p38 (loading control). (C) RPE1 cells expressing CCDC113-GFP were treated with DMSO or 10 µg/ml nocodazole, fixed, stained for GFP, PCM1 and γ-tubulin. (D) RPE1 cells expressing CCDC113–GFP were fixed 48 h after transfection with control (siCtl) or PCM1 (siPCM1) siRNA, and stained for GFP, PCM1 and γ-tubulin. Scale bars: 10 µm.
The punctate pericentrosomal distribution of satellite proteins is dependent on the satellite protein PCM1 and a centrosomally focused microtubule array (Kim et al., 2004; Kim et al., 2008; Lopes et al., 2011). CCDC113–GFP localized to a subset of PCM1-positive puncta in RPE1 cells (Fig. 3B,C), and these CCDC113-GFP- and PCM1-positive puncta dispersed upon depolymerization of microtubules (Fig. 3C), as would be expected for centriolar satellites. Moreover, depletion of PCM1 caused loss of CCDC113–GFP satellite localization (Fig. 3D). In both cases loss of CCDC113–GFP localization to satellites was accompanied by localization of the protein to the centrosome, as observed for some other satellite components (Stowe et al., 2012). These results identify CCDC113–GFP as a new component of centriolar satellites.
A high-throughput two-hybrid screen (Stelzl et al., 2005) identified an interaction between CCDC113 and the centriolar satellite protein HAP1 (Engelender et al., 1997; Keryer et al., 2011). We validated this interaction in co-immunoprecipitation experiments in HEK293T cells in which CCDC113 interacted with both HAP1 and PCM1 (Fig. 4A; supplementary material Fig. S2B,C). Moreover, CCDC113–GFP colocalized with HAP1 at centriolar satellites (Fig. 4B). HAP1 forms a complex with PCM1 and huntingtin and regulates ciliogenesis (Engelender et al., 1997; Keryer et al., 2011). We tested whether CCDC113 also is required for efficient ciliogenesis. CCDC113 was depleted from RPE1 cells by infection with a lentivirus expressing short hairpin RNA (shRNA) targeting CCDC113. Efficient depletion of CCDC113 was observed 7 d after infection, as demonstrated by immunoblotting for CCDC113 (supplementary material Fig. S2D). Depletion of CCDC113 substantially reduced cilium formation in serum-starved RPE1 cells, and this phenotype was rescued by expression of GFP-tagged mouse CCDC113 (Fig. 4C,D). The ciliogenesis phenotype was not due to a defect in cell cycle progression because both control and CCDC113-depleted cells had similar cell cycle profiles as analyzed by flow cytometry (supplementary material Fig. S2E). As with HAP1 (Keryer et al., 2011), depletion of CCDC113 caused dispersal of PCM1-positive satellites throughout the cytoplasm relative to control cells in which satellites were focused around the centrosome (Fig. 4E).
CCDC113 functions in ciliogenesis. (A) Co-immunoprecipitation of CCDC113–GFP, tomato–PCM1 (td-PCM1) and HAP1–Myc after co-expression in HEK293T cells. Complexes were immunoprecipitated with anti-GFP antibody and co-immunoprecipitated (IP) proteins were detected with anti-GFP, anti-Myc and anti-PCM1 antibodies. (B) RPE1 cells expressing HAP1–Myc and CCDC113–GFP were stained with anti-Myc and anti-GFP antibodies. DNA was stained with DAPI. (C) RPE1 cells were fixed 7 d after infection with virus expressing empty vector (shControl) or CCDC113 shRNA (shCCDC113), and the percentage of cells displaying ciliogenesis was determined by staining with polyglutamylated tubulin (glut. tubulin) and DAPI. (D) Quantification of C; n≥100 cells per experiment. **P<0.01, *P<0.05 (ANOVA). (E) Control and CCDC113-depleted RPE1 cells were fixed, and stained for PCM1. DNA was stained with DAPI. Scale bars: 10 µm. All insets are shown at 4× relative to the main image.
In this report, we used proteomic analysis of centrioles from a differentiated cell type, the mammalian sperm, to identify putative components of the centrosome in general. The combination of the unique properties of the sperm centrioles with the sperm centriole enrichment protocol yielded a particularly specific dataset of proteins that localize to the centrosome. Many of the most well characterized centrosome proteins are PCM components (Jakobsen et al., 2011). Thus, this dataset is likely to be particularly useful for studies of the centriole, about which little is known with respect to the relationship between structure (Li et al., 2012; Guichard et al., 2013) and specific proteins.
Eight of the 15 previously uncharacterized proteins tested in this study localize at or near the centrosome in cycling cells as GFP fusion proteins, validating the original assumption that the sperm centrioles have components in common with the centrosome. Except for the mother centriole protein CCDC146, most of the GFP-tagged proteins localized to a larger centrosome domain, mostly overlapping with the PCM marker γ-tubulin. This might reflect situations, such as with ODF2, in which the protein is part of morphologically distinct structures at the centrosome and in the sperm midpiece (Brohmann et al., 1997), or might be due to overexpression of the proteins, which can result in a broader distribution of centriolar proteins. Given the highly specialized structure and function of the sperm, we expect that the sperm centriole proteome would also include proteins specific to sperm. Indeed, several of these proteins have expressed-sequence tag (EST) expression profiles that are highly specific for testis (supplementary material Fig. S1B). For such proteins, the ability of the protein to localize to the somatic cell centrosome suggests that the protein or structure that it interacts with at the centrosome is common between sperm centrioles and cycling cell centrosomes.
Focusing on one of the sperm centriole candidates, we identified CCDC113 as a new component of centriolar satellites that is important for efficient ciliogenesis. We note that the pattern of evolutionary conservation of CCDC113 is similar to that for the satellite proteins BBS4 (Kim et al., 2004) and CEP290 (Kim et al., 2008), but not to that for PCM1 (Hodges et al., 2010). This suggests that the interaction of CCDC113 with satellites is likely to represent modulation of CCDC113 by satellites, rather than function of satellites. Consistent with this hypothesis, we found that elimination of satellites by PCM1 depletion results in relocalization of CCDC113 to the centrosome, suggesting that satellites sequester CCDC113 from the centrosome. Sperm lack the complement of satellites typical of most somatic cells, but a recent study (Hall et al., 2013) demonstrated that the satellite protein AZI1 (also known as CEP131) is required for sperm motility by regulating protein trafficking in sperm cells (Hall et al., 2013). CCDC113 and AZI1 might represent a class of proteins that have similar functions in sperm cells and somatic cells, but for which that function is regulated by association with satellites in somatic cells.
MATERIALS AND METHODS
Cell culture and transfection
HeLa and HEK293T cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS; Atlanta Biologicals, Lawrenceville, GA). RPE1 cells were grown in DMEM/F12 (50:50) supplemented with 10% FBS. Bovine sperm (Bovine Elite, College Station, TX) were used for centriole enrichment. RPE1 and HeLa cells were transfected using Lipofectamine LTX (Invitrogen). HEK293T cells were transfected using 1 µg/µl polyethylenimine (25 kDa) (Sigma-Aldrich, St. Louis, MO).
Sperm centriole enrichment
Bovine sperm were rinsed with PBS and sonicated to separate heads from tails, and sedimented through a 30% glycerol cushion to isolate the tails in the supernatant from the heads in the pellet. The tail fractions were sequentially extracted at 4°C for 1 h with extraction buffer 1 (50 mM Tris-HCl pH 8, 2 mM EGTA, 2 mM EDTA, 500 mM NaCl, 1% NP-40, 0.1% β-mercaptoethanol, 1 mM DTT, inhibitors), extraction buffer 2 (50 mM Tris-HCl pH 8, 600 mM KSCN, 2 mM DTT, inhibitors) and extraction buffer 3 (50 mM Tris-HCl pH 8, 4 M urea, inhibitors). The supernatant after each extraction was precipitated with trichloroacetic acid (TCA).
Plasmids
The GenBank, EMBL and DDBJ accession numbers and sources of all the cDNAs used in this study are described in supplementary material Fig. S1. The CCDC96 open reading frame (ORF) was amplified by PCR and cloned into peGFP-N1. The ORFs of the remaining genes were amplified by PCR and cloned into pDONR221 (Invitrogen). Subsequent Gateway recombination reactions using pCS2+6xMyc DEST and pcDNA-DEST47 were used to generate Myc- and/or GFP-tagged proteins.
RT-PCR and immunoprecipitation experiments
Total RNA was isolated from RPE1 cells using an RNeasy kit (Qiagen, Chatsworth, CA) and RT-PCR was performed using a cDNA synthesis kit (Invitrogen, San Diego, CA). Primers used were 5′-CGGAGCCTCTTATTCACTGC-3′ and 5′-AGCAAGCTTCACAAGGCAAT-3′. Co-immunoprecipitation experiments using HEK293T cells were performed as described previously (Firat-Karalar et al., 2014).
shRNA and rescue experiments
CCDC113 shRNA oligonucleotides (nucleotides 300–321, 5′-ATAGCAGAGATGTCCTTAAA-3′) were cloned into pLKO.1. CCDC113 depletion was carried out by infection of cells with shRNA-expressing lentivirus as described previously (Mahjoub et al., 2010). PCM1 was depleted using an small interfering RNA (siRNA) as described previously (Firat-Karalar et al., 2014). For rescue experiments, pools of RPE-1 cells stably expressing GFP (RPE-1::GFP) or mouse CCDC113–GFP (RPE-1::mCCDC113-GFP) were infected with lentivirus expressing empty vector or shRNA targeting CCDC113. Cells were serum starved for 48 h, fixed and stained on day 7 for the subsequent ciliogenesis assays.
Antibodies
Anti-CCDC146 antibody was obtained by immunizing rabbits (Cocalico Biologicals, Reamstown, PA) with GST-tagged CCDC146 (amino acids 1–461). The antibody was affinity purified against MBP–CCDC146 (1–461) and used at 0.5 µg/ml for immunofluorescence. Rabbit anti-p38 (C-20; Santa Cruz Biotechnology, Inc.) was used at 1:5000 for western blotting. Other antibodies used are described in supplementary material Fig. S1C.
Mass spectrometry
The centriole fraction was analyzed using an LCq-Deca-XPPlus mass spectrometer (Thermo Scientific) at the Stanford Mass Spectrometry Laboratory. The database was composed of Bos taurus protein sequences archived in the National Center for Biotechnology Information. Proteins were identified on the basis of at least two peptide matches with a probability >95%. The human orthologs of the B. taurus proteins for which annotation was not available were identified by reciprocal Blast analysis.
Immunofluorescence and microscopy
For immunofluorescence experiments, cells were grown on coverslips, fixed in methanol, processed for indirect immunofluorescence and imaged as described previously (Firat-Karalar et al., 2014). Sperm cells were dried on coverslips and processed for immunofluorescence as described for cell lines.
Footnotes
↵* Present address: Department of Molecular Biology and Genetics, Koç University, Istanbul 34450, Turkey.
↵‡ Present address: Division of Pulmonary and Critical Care, Stanford School of Medicine, Palo Alto, CA 94304, USA.
Competing interests
The authors declare no competing interests.
Author contributions
E.N.F-K., J.S. and S.E. performed the experiments; E.N.F-K. and T.S. interpreted the results, and E.N.F-K. and T.S. wrote the paper.
Funding
This work was supported a National Research Service Award [grant number 5F32GM106620 to E.N.F.]; and by the National Institutes of Health [grant number R01 GM52022 to T.S.]. Deposited in PMC for release after 12 months.
Supplementary material available online at http://jcs.biologists.org/lookup/suppl/doi:10.1242/jcs.157008/-/DC1
- Received June 23, 2014.
- Accepted July 23, 2014.
- © 2014. Published by The Company of Biologists Ltd
References
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Subject collections
Other journals from The Company of Biologists
Travelling Fellowship application deadline: 6 March
Don’t miss the 6 March deadline for the next round of Travelling Fellowships. For travel after 20 April 2020, early-career researchers can receive a grant of up to £2,500 to visit and collaborate with an international lab. Find out more and how to apply.
Cell scientist to watch – Jeremy Carlton
Meet Jeremy Carlton, a Wellcome Trust Senior Research Fellow, EMBO Young Investigator, leader of the Organelle Dynamics Laboratory and currently seconded to the Francis Crick Institute. In an interview, he tells us about his lab’s research, setting up his own group and his holidays on archaeological digs.
Welcoming early-career researchers to our Editorial Advisory Board
We are pleased to announce our latest endeavour to support the career development of scientists – the appointment of ECRs to our Editorial Advisory Board. Find out more about the initiative and the seven outstanding scientists.
Publishing peer review reports
To continue working towards transparency around the editorial process, JCS now publishes a ‘Peer review history file’ alongside published papers. Read more about the policy and see the reports for yourself in one of the first papers to take advantage of this initiative (under the ‘Info & metrics’ tab).
The lowdown on Read & Publish
The Company of Biologists is embracing Open Access with a series of pilot Transitional Agreements, known as Read & Publish deals. But, what exactly are they and what are the benefits? Read the full story to find out more.