IN THIS ISSUE
COMMENTARIES
- Autophagy in adhesion and migration
Summary: Discussion of emerging roles for selective autophagy in adhesion and migration through regulation of focal adhesion turnover, cellular tension and integrin trafficking.
- The changing view of eukaryogenesis – fossils, cells, lineages and how they all come together
Summary: Eukaryogenesis, the emergence of eukaryotic cells, represents a pivotal evolutionary event. Advances in paleogeology, phylogenetics, cell biology and microbial diversity provide new insights into aspects of eukaryogenesis.
RESEARCH ARTICLES
- Desmin and αB-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival
Highlighted Article: Both desmin and its partner chaperone αB-crystallin associate with mitochondria–sarcoplasmic-reticulum contact sites (MAMs), stabilizing MICOS super-complexes, and thus contributing to proper mitochondrial cristae structure–function.
- C. elegans midbodies are released, phagocytosed and undergo LC3-dependent degradation independent of macroautophagy
Summary: Autophagy proteins are required for the degradation of midbodies. In C. elegans, Atg8/LC3-family proteins act during phagosome maturation rather than during macroautophagy.
- Drosophila sensory cilia lacking MKS proteins exhibit striking defects in development but only subtle defects in adults
Highlighted Article: An analysis of Meckel–Gruber syndrome (MKS) fly mutants reveals that cilium structure is perturbed during development, but that cilia are largely structurally and functionally normal by adulthood.
- Triadin and CLIMP-63 form a link between triads and microtubules in muscle cells
Summary: CLIMP-63 is associated with the Ca2+ release complex of skeletal muscles through its interaction with triadin, and is involved in the shaping of triads and in the guidance of microtubules.
- Direct interaction between exocyst and Wave complexes promotes cell protrusions and motility
Highlighted Article: A direct link between exocyst and Wave complexes is revealed, providing new mechanistic insight into the spatio-temporal coordination between membrane trafficking and actin polymerization.
- Cdc48 and Ubx1 participate in a pathway associated with the inner nuclear membrane that governs Asi1 degradation
Summary: The inner nuclear membrane (INM) E3 ubiquitin ligase component Asi1 is ubiquitylated, removed from the membrane and targeted to nuclear proteasomes through a new INM-associated degradation pathway.
- Atg9A trafficking through the recycling endosomes is required for autophagosome formation
Highlighted Article: Atg9A trafficking through the recycling endosome is required for autophagy, and is mediated by TRAPPIII and intrinstic Atg9A-sorting motifs that bind to AP2.
- Prolyl endopeptidase is involved in the degradation of neural cell adhesion molecules in vitro
Summary: NCAM and its polysialylated form (PSA-NCAM) are important regulators of brain plasticity. We demonstrate that prolyl endopeptidase (PREP) is involved in the regulation of NCAM and PSA-NCAM.
- Mature lipid droplets are accessible to ER luminal proteins
Highlighted Article: Lipid droplets are accessible to ER luminal proteins, suggesting that lipid droplets form a specialized domain within the ER membrane that can be recognized from both the cytosolic and the luminal side.
- DUX4 induces a transcriptome more characteristic of a less-differentiated cell state and inhibits myogenesis
Summary: DUX4 underlies pathogenesis in facioscapulohumeral muscular dystrophy. DUX4 acts mainly as a transcriptional activator that inhibits myogenesis by orchestrating a gene expression profile representative of a more stem-cell-like state.
- Sortilin regulates sorting and secretion of Sonic hedgehog
Highlighted Article: We report a new role for sortilin (Sort1) as a negative regulator of Sonic hedgehog trafficking to axons and secretion from cells.
- The role of ADP-ribosylation in regulating DNA interstrand crosslink repair
Summary: Here, we identify a role for post-translational modification ADP-ribosylation in the response to DNA interstrand crosslinks in the model Dictyostelium.
- Endo-lysosomal TRP mucolipin-1 channels trigger global ER Ca2+ release and Ca2+ influx
Summary: The endolysosomal ion channel TRP mucolipin 1 was thought to mediate local Ca2+ signals. However, as reported here, it can also mediate global elevations in Ca2+.
- Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06
Summary: The secretion inhibitor FLI-06 inhibits recruitment of cargo into ERES by a mechanism upstream of Sar1 and COPII, and also blocks exit from the trans-Golgi network.
- Characterization of prion protein function by focal neurite stimulation
Summary: By using a novel approach to stimulate and guide the growth cones of hippocampal neurons with high spatial and temporal resolution, we reveal a function for the soluble form of PrP to promote fast neurite outgrowth.
- Essential role of the Dishevelled DEP domain in a Wnt-dependent human-cell-based complementation assay
Summary: A physiological complementation assay in Dishevelled null-mutant human cells establishes an essential function of the DEP domain of Dishevelled in its binding to Frizzled for signal transduction to β-catenin.
- Mutant IP3 receptors attenuate store-operated Ca2+ entry by destabilizing STIM–Orai interactions in Drosophila neurons
Summary: In Drosophila neurons, mutant IP3 receptors disrupt store-operated Ca2+ entry by destabilizing interaction of STIM with the Ca2+ channel, Orai. The interactions could coordinate store emptying with Ca2+ entry.
- Neuropilin-1 modulates interferon-γ-stimulated signaling in brain microvascular endothelial cells
Summary: This is the first study to define the role of neuropilin-1, a co-receptor of several structurally distinct cytokines, in the IFNγ-induced inflammatory response of endothelial cells in neuroinflammatory diseases.
- A ternary complex comprising transportin1, Rab8 and the ciliary targeting signal directs proteins to ciliary membranes
Summary: Transportin1 and Rab8 can simultaneously bind to the ciliary targeting signal in a guanine-nucleotide-dependent manner. The resulting ternary complex can target a membrane protein to cilia.
- Plant mitochondria contain the protein translocase subunits TatB and TatC
Summary: This paper provides evidence that Arabidopsis thaliana mitochondria contain two subunits of a twin arginine translocation (Tat) pathway, indicating the presence of an active Tat pathway in plants.
- Exosomes bind to autotaxin and act as a physiological delivery mechanism to stimulate LPA receptor signalling in cells
Summary: Autotaxin, a secreted enzyme that generates lysophosphatidic acid (LPA), binds to exosomes and is thereby targeted to cells in order to deliver LPA and activate cell surface receptors.
ARTICLES OF INTEREST IN OUR OTHER JOURNALS
From Development
This issue
JUMP TO
Other journals from The Company of Biologists
Cell scientist to watch – Vaishnavi Ananthanarayanan
Vaishnavi Ananthanarayanan is an Assistant Professor at the Centre for BioSystems Science and Engineering and the Indian Institute of Science. She also recently became an EMBO Young Investigator and Wellcome Trust/DBT – India Alliance Intermediate Fellow. Her research is focused on understanding dynein activation in living cells and the roles of microtubules in mitochondrial dynamics during neurodegeneration. She talks about her research, the importance of good mentors and much more.
Anna Akhmanova and Michel O. Steinmetz provide an overview of the current knowledge on the structure, interactions and functions of cellular factors that specifically interact with microtubule minus ends, and that regulate their dynamics and organization.
Mole - Dignity I
“What can we do to make dignity and respect part of what we do? Let's talk about that.”
Journal of Cell Science (JCS), Journal of Cell Biology (JCB) and Molecular Biology of the Cell (MBoC) announce a new manuscript transfer option that will empower authors whose manuscripts have been declined to seamlessly transfer their manuscripts and associated peer review reports between the journals.
Journal of Cell Science is pleased to welcome submissions for an upcoming special issue on 'Cell Biology of the Immune System' edited by Ana-Maria Lennon-Duménil. We invite you to showcase your breakthrough research on all aspects of cell biology as it relates to the immune system. The articles within this issue will receive extensive exposure to a broad audience of cell biologists and immunologists. Extended submission deadline: 15 August 2019.
preLights - Actin chromobody imaging reveals sub-organellar actin dynamics
Mariana De Niz highlights a preprint from Uri Manor and colleagues who use nanobody technology for live imaging of sub-organellar actin dynamics at previously unachieved temporal and spatial resolution, and explore its relevance for organelle fission.
Catch up with other recent preLights selected for the cell science community.
Articles of interest in our sister journals
A V0-ATPase-dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane
Aurélien Bidaud-Meynard, Ophélie Nicolle, Markus Heck, Yann Le Cunff, Grégoire Michaux
Development 2019 146: dev174508
Physical and functional cell-matrix uncoupling in a developing tissue under tension
Amsha Proag, Bruno Monier, Magali Suzanne
Development 2019 146: dev172577