IN THIS ISSUE
JCS PRIZE
CELL SCIENCE AT A GLANCE
- Regulators of actin filament barbed ends at a glance
Summary: Assembly of actin filaments at barbed ends drives motile processes. Here, we discuss the control of polarized filament assembly by various regulators that interact with and compete for barbed ends.
COMMENTARY
- The mechanical regulation of integrin–cadherin crosstalk organizes cells, signaling and forces
Summary: Multiple layers of crosstalk between integrins, cadherins and forces drive spatial organization of signaling and forces within cells.
RESEARCH ARTICLES
- SHIP2 controls plasma membrane PI(4,5)P2 thereby participating in the control of cell migration in 1321 N1 glioblastoma cells
Highlighted Article: SHIP2 modulates the balance between PI(4,5)P2 and PI4P, thereby participating in the control of migration in 1321 N1 glioblastoma cells through the organization of focal adhesions.
- Nuclear size is sensitive to NTF2 protein levels in a manner dependent on Ran binding
Summary: NTF2 reduces the diameter of the NPC, nuclear import of large cargos and nuclear size in a Ran-binding-dependent manner, in both Xenopus and mammalian cells.
- Correctors of mutant CFTR enhance subcortical cAMP–PKA signaling through modulating ezrin phosphorylation and cytoskeleton organization
Highlighted Article: Correctors of F508del CFTR, by activating ezrin and actin cytoskeleton re-organization, generate a sub-cortical pool of cAMP of adequate amplitude to activate chloride secretion.
- Translational control mediated by hnRNP K links NMHC IIA to erythroid enucleation
Summary: Post-transcriptional regulation of NMHC IIA protein expression by hnRNP K contributes to the process of enucleation in induced erythroid maturation.
- A tetraspanin regulates septate junction formation in Drosophila midgut
Highlighted Article: A tetraspanin family protein, Tsp2A is an essential component of septate junctions in the Drosophila endodermal epithelia and is involved in intestinal barrier function.
- Inhibition of the PI3K–Akt and mTORC1 signaling pathways promotes the elongation of vascular endothelial cells
Summary: PI3K–Akt and mTORC1 inhibitors induce angiogenic elongation of vascular endothelial cells without an excessive VEGF stimulation.
- Myosin Vb mediates Cu+ export in polarized hepatocytes
Summary: Myosin Vb regulates Cu+-induced recycling of ATP7B at the apical membrane in polarized hepatocytes.
- Proteasome storage granules are transiently associated with the insoluble protein deposit in Saccharomyces cerevisiae
Summary: Carbon source depletion in Saccharomyces cerevisiae leads to the triage of proteasome storage granules (PSGs) and dysfunctional proteasomes on the IPOD, to ensure that only structurally ‘correct’ proteasomes are deployed.
- Soluble LILRA3 promotes neurite outgrowth and synapses formation through a high-affinity interaction with Nogo 66
Summary: LILRA3 protein is a soluble new high-affinity ligand to Nogo 66 and promotes neurite outgrowth and synapse formation in primary cortical neurons by antagonising Nogo-66-mediated inhibition.
- Activator of G-protein signaling 8 is involved in VEGF-mediated signal processing during angiogenesis
Summary: Activator of G-protein signaling 8 (AGS8) regulates the angiogenic events in endothelial cells by influencing the intracellular distribution of VEGFR-2. A new signal input to angiogenesis by AGS8 is suggested.
- REV1 promotes PCNA monoubiquitylation through interacting with ubiquitylated RAD18
Summary: Monoubiquitylation (mUb) of PCNA is a crucial event coordinating DNA damage tolerance pathways. We report here that translesion synthesis polymerase REV1 can promote PCNA–mUb through its preferential association with RAD18–mUb.
- A new role for cofilin in retinal neovascularization
Summary: Pak1 and its downstream effector cofilin play an essential role in VEGFA-induced angiogenic events in human retinal microvascular endothelial cells in vitro and hypoxia-induced retinal neovascularization in vivo.
- Nuclear envelope expansion is crucial for proper chromosomal segregation during a closed mitosis
Summary: Identification of a new fatty acid synthase inhibitor for nuclear division by a chemical genetic screen revealed a link between nuclear envelope expansion and faithful chromosome segregation in a closed mitosis.
TOOLS AND TECHNIQUES
- Distinguishing aggregate formation and aggregate clearance using cell-based assays
Highlighted Article: Disequilibrium between aggregate formation and clearance leads to accumulation of aggregated proteins. We have created a new cell-based assay that can determine how modifiers can impact on aggregate burden.
- DAZL and CPEB1 regulate mRNA translation synergistically during oocyte maturation
Highlighted Article: The RNA-binding proteins DAZL and CPEB1 cooperate to regulate mRNA translation and protein synthesis during the meiotic cell cycle in mouse oocytes.
CORRECTIONS
ARTICLES OF INTEREST IN OTHER COB JOURNALS
This issue
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Other journals from The Company of Biologists
Editorial – The good, the bad and the very useful – at least to cell biologists
Editor-in-Chief, Michael Way, discusses the exciting potential of examining the intimate relationship between pathogens, cells and hosts for cell biology research. Submissions are welcome for the fifth JCS Special Issue, guest edited by Derek Walsh, focussing on host-pathogen interactions.
Cell Science at a Glance – The fusogenic synapse at a glance
In their Cell Science at a Glance, Ji Hoon Jim and Elizabeth H. Chen highlight the molecular, cellular and biophysical events at the asymmetric fusogenic synapse.
Review – Force-generating mechanisms of anaphase in human cells
Kruno Vukušić, Renata Buđa and Iva M. Tolić review contributions of force-generating mechanisms, acting through different microtubule populations, to chromosome segregation in human cells.
First Person interviews
Have you seen our First Person interviews with the early-career first authors of our papers? The authors talk about their work in and out of the lab, the journeys that led them to where they are now and the scientists who inspired them along the way. Recently, we caught up with first authors Bouchra Khalaf, Bapi Sarker, Amrit Bagchi and Tak Shun Fung.
Mole – Impact! III
“Over the last five years, there were 1,141,540 papers published per year that were listed in PubMed. If you read them for 8 h per day, 5 days week, for 50 weeks year, you would have to read about ten papers a minute to read them all.”
Read Mole’s thoughts on the issue of impact in scientific literature.
preLights – preLights reaches 500 posts!
The success of preLights is down to the hard work of our preLighters. Head over to the preLights website to find out more about our most active early-career-researchers.
Journal Meeting – Cell Dynamics: Host-Pathogen Interface
Registration is now open for the third instalment of the highly successful Cellular Dynamics Meeting Series, which will take place on 17-20 May 2020 and will focus on ‘Host-Pathogen Interface’.
Articles of interest in our sister journals
Scribble and Discs Large mediate tricellular junction formation
Zohreh Sharifkhodaei, Mary M. Gilbert, Vanessa J. Auld (Development 2019 146: dev174763)
Annabel Ebbing, Teije C. Middelkoop, Marco C. Betist, Eduard Bodewes, Hendrik C. Korswagen (Development 2019 146: dev180059)