IN THIS ISSUE
FIRST PERSON
CELL SCIENCE AT A GLANCE
- Targeting and translocation of proteins to the endoplasmic reticulum at a glance
Summary: Targeting and translocation into the ER are mediated by multiple pathways, which together enable the correct localization of about 30% of any eukaryotic proteome.
REVIEW
- The EGFR odyssey – from activation to destruction in space and time
Summary: We review the molecular events that occur during EGFR activation, its trafficking through the endosomal system and its subsequent downregulation.
RESEARCH ARTICLES
- Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin
Highlighted Article: During the morula stage in sea urchin embryos, early histone gene loci undergo interchromosomal interactions that are dependent on the active transcriptional status.
- Functional analyses of the CIF1–CIF2 complex in trypanosomes identify the structural motifs required for cytokinesis
Summary: The trypanosome CIF1 zinc-finger motifs are essential for CIF1–CIF2 complex assembly, and along with the coiled-coil motifs in CIF1 are required for initiation of cytokinesis.
- Effects of mutating α-tubulin lysine 40 on sensory dendrite development
Highlighted Article: Neurons are enriched in post-translationally modified microtubules. Targeted mutagenesis of endogenous α-tubulin in flies reveals that dendrite branch refinement is altered by acetylation-blocking mutations.
- Giantin-knockout models reveal a feedback loop between Golgi function and glycosyltransferase expression
Summary: Knockout of giantin in a genome-engineered cell line and zebrafish models reveals the capacity of the Golgi to control its own biochemistry through changes in gene expression.
- Phosphorylation of the RNA-binding protein Zfs1 modulates sexual differentiation in fission yeast
Summary: The RNA-binding protein Zfs1 regulates accumulation of the G1 cyclin Puc1, a process that is modulated by phosphorylation. This regulatory mechanism has an important impact on cell differentiation.
- CREB3L1-mediated functional and structural adaptation of the secretory pathway in hormone-stimulated thyroid cells
Summary: CREB3L1 has a role during the adaptation of the secretory pathway in response to high secretory demand in thyroid cells, a process with direct impact on thyroid physiology.
- RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis
Summary: RhoC accumulates at phagocytic cups and modulates an mDia1-dependent remodeling of cortical F-actin that is required for FcγR-mediated phagocytosis in macrophages.
- Quantitative analysis of multilayer organization of proteins and RNA in nuclear speckles at super resolution
Summary: Multi-color structured illumination microscopy imaging studies reveal a multilayer organization of nuclear speckles due to the interplay between favorable sequence-encoded intermolecular interactions of speckle-resident proteins and RNAs.
TOOLS AND RESOURCES
- In situ imaging of mitochondrial translation shows weak correlation with nucleoid DNA intensity and no suppression during mitosis
Highlighted Article: Imaging de novo mitochondrial protein synthesis with a novel fluorescence method shows the translational output throughout the cell and reveals a low correlation with mitochondrion genome copy number.
- ImaEdge – a platform for quantitative analysis of the spatiotemporal dynamics of cortical proteins during cell polarization
Summary: An open-source software package, ImaEdge, automates the segmentation of the cell cortex from time-lapse movies, and enables quantitative extraction of cortical protein dynamics in cells undergoing polarization.
- Real-time observation of light-controlled transcription in living cells
Summary: Blue light-induced chromatin recruitment (BLInCR) enables rapid and reversible targeting of effector proteins to nuclear loci with high temporal resolution for analyzing transcription activation.
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JCS and COVID-19
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Ricardo Henriques introduces FocalPlane
FocalPlane Scientific Advisory Board member Ricardo Henriques (University College London) gives a brief introduction to our upcoming microscopy community site. Follow us on Twitter @focalplane_jcs or sign up for the latest launch updates.
The Corona Files
“…if there is one thing that is achingly, chillingly clear, if it wasn't already, is that this thing we do is really, really important.”
Our resident insectivore, Mole, has started a new series – The Corona Files. Follow Mole’s weekly musings on how COVID-19 is changing the landscape for researchers.
Do you have an idea for a Workshop?
Our Workshop programme may be on hold for now, but we are welcoming ideas for the 2022 line-up. The proposal deadline is 29 May 2020, so find out how to pitch your idea to the Workshop Committee in good time.
Call for papers: Cell Biology of Host-Pathogen Interactions
Showcase your breakthrough research on all aspects of cell biology relating to host-pathogen interactions for our upcoming Special Issue. Articles must be received by 30 July 2020 for consideration. Find out more about the scope, publication schedule and how to submit your manuscript.