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Research Article
Role for the splicing factor TCERG1 in Cajal body integrity and snRNP assembly
Cristina Moreno-Castro, Silvia Prieto-Sánchez, Noemí Sánchez-Hernández, Cristina Hernández-Munain, Carlos Suñé
Journal of Cell Science 2019 132: jcs232728 doi: 10.1242/jcs.232728 Published 14 November 2019

ABSTRACT

Cajal bodies are nuclear organelles involved in the nuclear phase of small nuclear ribonucleoprotein (snRNP) biogenesis. In this study, we identified the splicing factor TCERG1 as a coilin-associated factor that is essential for Cajal body integrity. Knockdown of TCERG1 disrupts the localization of the components of Cajal bodies, including coilin and NOLC1, with coilin being dispersed in the nucleoplasm into numerous small foci, without affecting speckles, gems or the histone locus body. Furthermore, the depletion of TCERG1 affects the recruitment of Sm proteins to uridine-rich small nuclear RNAs (snRNAs) to form the mature core snRNP. Taken together, the results of this study suggest that TCERG1 plays an important role in Cajal body formation and snRNP biogenesis.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: C.M.-C., S.P.-S., C.H.-M., C.S.; Methodology: C.M.-C., S.P.-S.; Validation: C.M.-C.; Formal analysis: C.M.-C.; Investigation: C.M.-C., N.S.-H.; Resources: S.P.-S., C.H.-M., C.S.; Writing - original draft: C.M.-C., C.S.; Writing - review & editing: C.H.-M., C.S.; Visualization: C.M.-C., S.P.-S., C.S.; Supervision: C.H.-M., C.S.; Project administration: C.S.; Funding acquisition: C.H.-M., C.S.

  • Funding

    This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (Ministerio de Economía y Competitividad; grant number BFU2017-89179-R to C.S. and grant number BFU2016-79699-P to C.H.M.) and the Andalusian Government (Excellence Project BIO-2515/2012) to C.S. Support from the European Region Development Fund [ERDF (FEDER)] is also acknowledged.

  • Supplementary information

    Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.232728.supplemental

  • Received April 3, 2019.
  • Accepted October 11, 2019.
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Research Article
Role for the splicing factor TCERG1 in Cajal body integrity and snRNP assembly
Cristina Moreno-Castro, Silvia Prieto-Sánchez, Noemí Sánchez-Hernández, Cristina Hernández-Munain, Carlos Suñé
Journal of Cell Science 2019 132: jcs232728 doi: 10.1242/jcs.232728 Published 14 November 2019
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