ABSTRACT
Cajal bodies are nuclear organelles involved in the nuclear phase of small nuclear ribonucleoprotein (snRNP) biogenesis. In this study, we identified the splicing factor TCERG1 as a coilin-associated factor that is essential for Cajal body integrity. Knockdown of TCERG1 disrupts the localization of the components of Cajal bodies, including coilin and NOLC1, with coilin being dispersed in the nucleoplasm into numerous small foci, without affecting speckles, gems or the histone locus body. Furthermore, the depletion of TCERG1 affects the recruitment of Sm proteins to uridine-rich small nuclear RNAs (snRNAs) to form the mature core snRNP. Taken together, the results of this study suggest that TCERG1 plays an important role in Cajal body formation and snRNP biogenesis.
Footnotes
Competing interests
The authors declare no competing or financial interests.
Author contributions
Conceptualization: C.M.-C., S.P.-S., C.H.-M., C.S.; Methodology: C.M.-C., S.P.-S.; Validation: C.M.-C.; Formal analysis: C.M.-C.; Investigation: C.M.-C., N.S.-H.; Resources: S.P.-S., C.H.-M., C.S.; Writing - original draft: C.M.-C., C.S.; Writing - review & editing: C.H.-M., C.S.; Visualization: C.M.-C., S.P.-S., C.S.; Supervision: C.H.-M., C.S.; Project administration: C.S.; Funding acquisition: C.H.-M., C.S.
Funding
This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (Ministerio de Economía y Competitividad; grant number BFU2017-89179-R to C.S. and grant number BFU2016-79699-P to C.H.M.) and the Andalusian Government (Excellence Project BIO-2515/2012) to C.S. Support from the European Region Development Fund [ERDF (FEDER)] is also acknowledged.
Supplementary information
Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.232728.supplemental
- Received April 3, 2019.
- Accepted October 11, 2019.
- © 2019. Published by The Company of Biologists Ltd
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