Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact the journal
    • Subscriptions
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact the journal
    • Subscriptions
    • Advertising
    • Feedback
Research Article
The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes
Xiaolei Gao, Marjorie Schmid, Ying Zhang, Sayumi Fukuda, Norio Takeshita, Reinhard Fischer
Journal of Cell Science 2019 132: jcs234799 doi: 10.1242/jcs.234799 Published 13 December 2019
Xiaolei Gao
Karlsruhe Institute of Technology (KIT) - South Campus, Institute for Applied Biosciences, Dept. of Microbiology, Fritz-Haber-Weg 4, D-76131 Karlsruhe, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Marjorie Schmid
Karlsruhe Institute of Technology (KIT) - South Campus, Institute for Applied Biosciences, Dept. of Microbiology, Fritz-Haber-Weg 4, D-76131 Karlsruhe, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Zhang
Karlsruhe Institute of Technology (KIT) - South Campus, Institute for Applied Biosciences, Dept. of Microbiology, Fritz-Haber-Weg 4, D-76131 Karlsruhe, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sayumi Fukuda
Tsukuba University, Faculty of Life and Environmental Sciences, Tsukuba 305-8572, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norio Takeshita
Tsukuba University, Faculty of Life and Environmental Sciences, Tsukuba 305-8572, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Reinhard Fischer
Karlsruhe Institute of Technology (KIT) - South Campus, Institute for Applied Biosciences, Dept. of Microbiology, Fritz-Haber-Weg 4, D-76131 Karlsruhe, Germany
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Reinhard Fischer
  • For correspondence: reinhard.fischer@KIT.edu
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF
Loading

ABSTRACT

Centrosomes are important microtubule-organizing centers (MTOCs) in animal cells. In addition, non-centrosomal MTOCs (ncMTOCs) are found in many cell types. Their composition and structure are only poorly understood. Here, we analyzed nuclear MTOCs (spindle-pole bodies, SPBs) and septal MTOCs in Aspergillus nidulans. They both contain γ-tubulin along with members of the family of γ-tubulin complex proteins (GCPs). Our data suggest that SPBs consist of γ-tubulin small complexes (γ-TuSCs) at the outer plaque, and larger γ-tubulin ring complexes (γ-TuRC) at the inner plaque. We show that the MztA protein, an ortholog of the human MOZART protein (also known as MZT1), interacted with the inner plaque receptor PcpA (the homolog of fission yeast Pcp1) at SPBs, while no interaction nor colocalization was detected between MztA and the outer plaque receptor ApsB (fission yeast Mto1). Septal MTOCs consist of γ-TuRCs including MztA but are anchored through AspB and Spa18 (fission yeast Mto2). MztA is not essential for viability, although abnormal spindles were observed frequently in cells lacking MztA. Quantitative PALM imaging revealed unexpected dynamics of the protein composition of SPBs, with changing numbers of γ-tubulin complexes over time during interphase and constant numbers during mitosis.

This article has an associated First Person interview with the first author of the paper.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: R.F.; Investigation: X.G., M.S., Y.Z., S.F., N.T.; Data curation: X.G.; Writing - original draft: X.G.; Writing - review & editing: R.F.; Supervision: R.F.; Project administration: R.F.; Funding acquisition: R.F.

  • Funding

    This work was supported by the German Science Foundation (Deutsche Forschungsgemeinschaft; DFG), grant number Fi459/20-1, and the Japanese Science and Technology Agency (JST) ERATO (grant number JPMJER1502). X.G. was a fellow of the Chinese Scholarship Council (CSC).

  • Supplementary information

    Supplementary information available online at http://jcs.biologists.org/lookup/doi/10.1242/jcs.234799.supplemental

  • Received May 28, 2019.
  • Accepted October 28, 2019.
  • © 2019. Published by The Company of Biologists Ltd
http://www.biologists.com/user-licence-1-1/
View Full Text

Log in using your username and password

Forgot your user name or password?

Log in through your institution

You may be able to gain access using your login credentials for your institution. Contact your library if you do not have a username and password.
If your organization uses OpenAthens, you can log in using your OpenAthens username and password. To check if your institution is supported, please see this list. Contact your library for more details.

Pay Per Article - You may access this article (from the computer you are currently using) for 1 day for US$30.00 .

Regain Access - You can regain access to a recent Pay per Article purchase if your access period has not yet expired.

Previous ArticleNext Article
Back to top
Previous ArticleNext Article

This Issue

Keywords

  • Aspergillus
  • Microtubules
  • γ-tubulin
  • MTOC
  • SPB
  • GCP
  • MOZART

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
Share
Research Article
The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes
Xiaolei Gao, Marjorie Schmid, Ying Zhang, Sayumi Fukuda, Norio Takeshita, Reinhard Fischer
Journal of Cell Science 2019 132: jcs234799 doi: 10.1242/jcs.234799 Published 13 December 2019
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Research Article
The spindle pole body of Aspergillus nidulans is asymmetrical and contains changing numbers of γ-tubulin complexes
Xiaolei Gao, Marjorie Schmid, Ying Zhang, Sayumi Fukuda, Norio Takeshita, Reinhard Fischer
Journal of Cell Science 2019 132: jcs234799 doi: 10.1242/jcs.234799 Published 13 December 2019

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
    • ABSTRACT
    • INTRODUCTION
    • RESULTS
    • DISCUSSION
    • MATERIALS AND METHODS
    • Acknowledgements
    • Footnotes
    • References
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • Concerted regulation of actin polymerization during constitutive secretion by cortactin and PKD2
  • Melanoma mutations modify melanocyte dynamics in co-culture with keratinocytes or fibroblasts
Show more RESEARCH ARTICLE

Similar articles

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

ASCBǀEMBO 2019 Special Collection

We're at ASCB - visit booth 1000 to meet the JCS team!
Enjoy a collection of articles published in Journal of Cell Science by a range of speakers at the ASCBǀEMBO 2019 Meeting. Featuring articles from the labs of JCS Editorial Advisory Board members, all articles in the collection are free to access.


Early-bird meeting deadline - 20 December

Wotton House

Don’t miss the early-bird application deadline for the 2020 JCS meeting on Host-Pathogen interface! Taking place 17-20 May 2020 at Wotton House, Surrey, UK, the meeting will bring together experts working at the interface between cell biology and pathogens. Places are limited, so apply to attend now.


Cell Scientist to Watch – Elizabeth Hinde

Elizabeth with her daughter and father.

From physics and chemistry to art and back again, Elizabeth Hinde is currently based at the University of Melbourne. Her research focuses on fluorescence microscopy methods to quantify live-cell nuclear organisation and the role chromatin dynamics play in maintaining genome function. Read the full interview to find out more. 


Have you heard about our Travelling Fellowships?

Huw and colleagues from the lab in Beijing

Early-career researchers can apply for up to £2,500 to offset the cost of travel and expenses to make collaborative visits to other labs around the world. Read about Huw’s experience in Beijing, where he spent time with the world leaders in the development of super-resolution microscopy, the Li lab at the Chinese Academy of Sciences.


Articles of interest in our sister journals

Casein kinase 1α decreases β-catenin levels at adherens junctions to facilitate wound closure in Drosophila larvae
Chang-Ru Tsai, Michael J. Galko
Development

Spherical spindle shape promotes perpendicular cortical orientation by preventing isometric cortical pulling on both spindle poles during C. elegans female meiosis
Elizabeth Vargas, Karen P. McNally, Daniel B. Cortes, Michelle T. Panzica, Brennan M. Danlasky, Qianyan Li, Amy Shaub Maddox, Francis J. McNally
Development

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Fast-track manuscripts
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Manuscript transfer network
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Journal of Cell Science
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2019   The Company of Biologists Ltd   Registered Charity 277992