Article Figures & Tables
Figures
- Fig. 1.
The presence and localization of tau in Schwann cells. (A) Typical amplification curves of real-time RT-PCR examining the expression of mRNAs coding for tau protein (Mapt) in Schwann cells and cerebral cortex. Although the expression of mRNAs coding for tau protein in the cerebral cortex is much higher than that of Schwann cells, there is indeed tau expression in Schwann cells. (B) Summarized Ct values for Gapdh and Mapt in Schwann cells and cerebral cortex. (C) DNA gel image of Mapt and Gapdh in Schwann cells and cerebral cortex. (D) Tau protein expression in Schwann cells (SCs) and cerebral cortex. This indicates that there is indeed tau protein expression in Schwann cells. (E) DNA gel image of genes coding for 3R and 4R tau isoforms in Schwann cells. (F) Localization of tau protein in cultured primary Schwann cells. Red indicates S100β and green indicates tau protein. (G) Localization of tau protein in rat sciatic nerve stumps. Green indicates S100β and red indicates tau protein. Scale bars: 50 μm.
- Fig. 2.
Tau affects Schwann cell proliferation. (A) The mRNA and (B) protein expression levels of tau in Schwann cells were decreased by Mapt siRNA transfection (MAPT-siRNA) as compared to siRNA control (NC-siRNA). (C) Transfection of Schwann cells with Mapt siRNA, compared with siRNA control, increased Schwann cell proliferation. Scale bars: 100 μm. (D) Schwann cells isolated from Mapt-knockout mice (Tau KO), compared with wild-type mice, showed elevated cell proliferation. In C and D, the magenta color indicates EdU staining and blue indicates Hoechst 33342 staining.
- Fig. 3.
Tau affects Schwann cell migration. (A) Transfection of Schwann cells with Mapt siRNA (MAPT-siRNA) decreased Schwann cell migration compared with transfection of siRNA control (NC-siRNA). (B) Schwann cells isolated from Mapt-knockout mice (Tau KO), compared with wild-type mice, showed reduced cell migration. See Materials and Methods for a detailed description of the assay. *P<0.05. Scale bars: 50 μm.
- Fig. 4.
Tau affects Schwann cell differentiation. (A) The mRNA levels of MBP, P0 and Mapt were higher in Schwann cells cultured in differentiation culture medium (db-cAMP+HRG) than those cultured in control medium. (B) The mRNA levels of Mbp, P0 and Mapt were higher in siRNA control (NC-siRNA)-transfected Schwann cells cultured in differentiation culture medium than Mapt siRNA-transfected Schwann cells (MAPT-siRNA) cultured in differentiation culture medium. *P<0.05.
- Fig. 5.
Tau affects Schwann cell migration in vivo. (A) Tau immunoblotting in the sciatic nerve stumps of rats injected with siRNA control (NC-siRNA) and rats injected with Mapt siRNA (MAPT-siRNA). Scale bar: 100 μm. (B) The in vivo migration of Schwann cells was impaired in rats injected with Mapt siRNA as compared with rats injected with siRNA control. Immunostaining with anti-S100β was performed at 4 days post rat sciatic nerve crush and was used to determine Schwann cell migration. The area with the dashed line indicates the crush area, and the areas set as the proximal, distal and middle site are indicated. Boxed areas are shown at a higher magnification on the right. Scale bars: 200 μm (main image), 20 μm (magnification). The relative fluorescence intensity (calcaulated with Image Analysis System Software, Leica) of S100β at the proximal, middle and distal sites were summarized and shown as a histogram. (C) Tau immunostaining (green) in the sciatic nerve stumps of wild-type mice and Mapt-knockout mice (Tau KO). DAPI staining is shown in blue. Scale bar: 100 μm. (D) The in vivo Schwann cell migration in Mapt-knockout mice was slower than in wild-type mice. Immunostaining with anti-S100β (red) was performed at 3 days post mouse sciatic nerve crush and was used to determine Schwann cell migration as in B. The area with the dashed line indicates the crush area, and the areas set as the proximal, distal and middle site are indicated. Boxed areas are shown at a higher magnification on the right. Scale bars: 100 μm (main image), 10 μm (magnification). *P<0.05.
- Fig. 6.
Tau affects debris clearance. (A) The amount of remaining myelin and lipid debris was higher in rats injected with Mapt siRNA (MAPT-siRNA) as compared with rats injected with siRNA control (NC-siRNA). (B) The amount of remaining myelin and lipid debris was higher in Mapt-knockout mice (Tau KO) as compared with wild-type mice. Scale bars: 50 μm. Images show Oil Red O staining.
- Fig. 7.
Tau affects cytoskeleton proteins. (A) Immunostaining of S100β, α-tubulin and DAPI in Schwann cells isolated from wild-type mice and Mapt-knockout mice (Tau KO). (B) Immunostaining of S100β, F-actin and DAPI in Schwann cells isolated from wild-type mice and Mapt-knockout mice. Scale bars: 20 μm.
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
Introducing FocalPlane’s new Community Manager, Esperanza Agullo-Pascual
We are pleased to welcome Esperanza to the Journal of Cell Science team. The new Community Manager for FocalPlane, Esperanza is joining us from the Microscopy Core at Mount Sinai School of Medicine. Find out more about Esperanza in her introductory post over on FocalPlane.
New funding scheme supports sustainable events
As part of our Sustainable Conferencing Initiative, we are pleased to announce funding for organisers that seek to reduce the environmental footprint of their event. The next deadline to apply for a Scientific Meeting grant is 26 March 2021.
Read & Publish participation continues to grow
"Alongside pre-printing for early documentation of work, such mechanisms are particularly helpful for early-career researchers like me.”
Dr Chris MacDonald (University of York) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 150 institutions in 15 countries and four library consortia taking part – find out more and view our full list of participating institutions.
Cell scientist to watch: Romain Levayer
In an interview, Romain Levayer talks about starting his own lab, his love for preprints and his experience of balancing parenting with his research goals.
Live lactating mammary tissue
In a stunning video, Stewart et al. demonstrate warping of the alveolar unit due to basal cell-generated force as part of their recent work investigating roles for mechanically activated ion channels in lactation and involution.
Visit our YouTube channel to watch more videos from JCS, our sister journals and the Company.
JCS and COVID-19
For more information on measures Journal of Cell Science is taking to support the community during the COVID-19 pandemic, please see here.
If you have any questions or concerns, please do not hestiate to contact the Editorial Office.