Cover image

Cover: Winning image, ‘Owl Eyes’, from the 2020 FocalPlane image competition, taken by Tejeshwar Rao from Alexa L. Mattheyses’ lab at the University of Alabama, USA. The image depicts a duplicated Cos-7 cell plated on a tension gauge tether surface. After stimulation with epidermal growth factor (EGF), the cell was fixed and stained for F-actin (orange hot) using phalloidin and for focal adhesions (blue-green) using a paxillin antibody. Integrin forces (i.e. open tension probes) are shown in greyscale. Total internal reflection fluorescence (TIRF) microscopy was employed to acquire the images, which were processed using NIS Elements and reconstructed in Fiji (ImageJ).