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Research Article
The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis
Wei Sheng Yap, Peter Shyu, Jr, Maria Laura Gaspar, Stephen A. Jesch, Charlie Marvalim, William A. Prinz, Susan A. Henry, Guillaume Thibault
Journal of Cell Science 2020 133: jcs248526 doi: 10.1242/jcs.248526 Published 5 November 2020
Wei Sheng Yap
1School of Biological Sciences Nanyang Technological University, Singapore, 637551
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Peter Shyu Jr
1School of Biological Sciences Nanyang Technological University, Singapore, 637551
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Maria Laura Gaspar
2Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
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Stephen A. Jesch
2Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
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Charlie Marvalim
1School of Biological Sciences Nanyang Technological University, Singapore, 637551
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William A. Prinz
3Laboratory of Cell and Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, Bethesda, MD 20892, USA
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  • ORCID record for William A. Prinz
Susan A. Henry
2Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA
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  • ORCID record for Susan A. Henry
Guillaume Thibault
1School of Biological Sciences Nanyang Technological University, Singapore, 637551
4Institute of Molecular and Cell Biology, A*STAR, Singapore, 138673
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  • ORCID record for Guillaume Thibault
  • For correspondence: thibault@ntu.edu.sg

Handling Editor: David Stephens

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ABSTRACT

Lipid droplets (LDs) are implicated in conditions of lipid and protein dysregulation. The fat storage-inducing transmembrane (FIT; also known as FITM) family induces LD formation. Here, we establish a model system to study the role of the Saccharomyces cerevisiae FIT homologues (ScFIT), SCS3 and YFT2, in the proteostasis and stress response pathways. While LD biogenesis and basal endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) remain unaltered in ScFIT mutants, SCS3 was found to be essential for proper stress-induced UPR activation and for viability in the absence of the sole yeast UPR transducer IRE1. Owing to not having a functional UPR, cells with mutated SCS3 exhibited an accumulation of triacylglycerol within the ER along with aberrant LD morphology, suggesting that there is a UPR-dependent compensatory mechanism that acts to mitigate lack of SCS3. Additionally, SCS3 was necessary to maintain phospholipid homeostasis. Strikingly, global protein ubiquitylation and the turnover of both ER and cytoplasmic misfolded proteins is impaired in ScFITΔ cells, while a screen for interacting partners of Scs3 identifies components of the proteostatic machinery as putative targets. Together, our data support a model where ScFITs play an important role in lipid metabolism and proteostasis beyond their defined roles in LD biogenesis.

This article has an associated First Person interview with the first author of the paper.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: G.T.; Methodology: W.S.Y., P.S., M.L.G., C.M., S.A.H., G.T.; Formal analysis: W.S.Y., P.S., M.L.G.; Validation: W.S.Y., P.S., M.L.G.; Investigation: W.S.Y., P.S., M.L.G., S.A.J., C.M.; Resources: W.S.Y., P.S., M.L.G.; Visualization: W.S.Y., P.S., G.T.; Writing - original draft: P.S., G.T.; Writing - review & editing: W.S.Y., M.L.G., G.T.; Supervision: G.T.; Project administration: G.T.; Funding acquisition: P.S., W.A.P., S.A.H., G.T.

  • Funding

    This work was supported by the Nanyang Assistant Professorship programme from the Nanyang Technological University to G.T., the National Research Foundation, Singapore, under its NRF-NSFC joint research grant call (Synthetic Biology, NRF2018NRFNSFC003SB-006) to G.T., the Nanyang Technological University Research Scholarship to P.J.S. (predoctoral fellowship), the National Institutes of Health (NIH) grant GM-19629 to S.A.H., the Intramural Research Program of the NIH to W.A.P., The National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) to W.A.P. Deposited in PMC for release after 12 months.

  • Data availability

    The original data for this study are available at the Dataverse Project doi:10.21979/N9/I7MXVP.

  • Supplementary information

    Supplementary information available online at https://jcs.biologists.org/lookup/doi/10.1242/jcs.248526.supplemental

  • Received May 5, 2020.
  • Accepted October 1, 2020.
  • © 2020. Published by The Company of Biologists Ltd
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Keywords

  • Endoplasmic reticulum-associated degradation
  • ERAD
  • Lipid droplet
  • Proteostasis
  • Phospholipid metabolism
  • Scs3
  • Unfolded protein response
  • UPR

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Research Article
The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis
Wei Sheng Yap, Peter Shyu, Jr, Maria Laura Gaspar, Stephen A. Jesch, Charlie Marvalim, William A. Prinz, Susan A. Henry, Guillaume Thibault
Journal of Cell Science 2020 133: jcs248526 doi: 10.1242/jcs.248526 Published 5 November 2020
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Research Article
The yeast FIT2 homologs are necessary to maintain cellular proteostasis and membrane lipid homeostasis
Wei Sheng Yap, Peter Shyu, Jr, Maria Laura Gaspar, Stephen A. Jesch, Charlie Marvalim, William A. Prinz, Susan A. Henry, Guillaume Thibault
Journal of Cell Science 2020 133: jcs248526 doi: 10.1242/jcs.248526 Published 5 November 2020

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