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Research Article
A novel coordinated function of Myosin II with GOLPH3 controls centralspindlin localization during cytokinesis in Drosophila
Stefano Sechi, Anna Frappaolo, Angela Karimpour-Ghahnavieh, Roberta Fraschini, Maria Grazia Giansanti
Journal of Cell Science 2020 133: jcs252965 doi: 10.1242/jcs.252965 Published 10 November 2020
Stefano Sechi
1Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Biologia e Biotecnologie, Università Sapienza di Roma, Piazzale A. Moro 5, 00185 Roma, Italy
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Anna Frappaolo
1Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Biologia e Biotecnologie, Università Sapienza di Roma, Piazzale A. Moro 5, 00185 Roma, Italy
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Angela Karimpour-Ghahnavieh
1Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Biologia e Biotecnologie, Università Sapienza di Roma, Piazzale A. Moro 5, 00185 Roma, Italy
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Roberta Fraschini
2Dipartimento di Biotecnologie e Bioscienze, Università degli Studi di Milano Bicocca, 20126, Milano, Italy
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Maria Grazia Giansanti
1Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Biologia e Biotecnologie, Università Sapienza di Roma, Piazzale A. Moro 5, 00185 Roma, Italy
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  • ORCID record for Maria Grazia Giansanti
  • For correspondence: mariagrazia.giansanti@uniroma1.it

Handling Editor: David Glover

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ABSTRACT

In animal cell cytokinesis, interaction of non-muscle myosin II (NMII) with F-actin provides the dominant force for pinching the mother cell into two daughters. Here we demonstrate that celibe (cbe) is a missense allele of zipper, which encodes the Drosophila Myosin heavy chain. Mutation of cbe impairs binding of Zipper protein to the regulatory light chain Spaghetti squash (Sqh). In dividing spermatocytes from cbe males, Sqh fails to concentrate at the equatorial cortex, resulting in thin actomyosin rings that are unable to constrict. We show that cbe mutation impairs localization of the phosphatidylinositol 4-phosphate [PI(4)P]-binding protein Golgi phosphoprotein 3 (GOLPH3, also known as Sauron) and maintenance of centralspindlin at the cell equator of telophase cells. Our results further demonstrate that GOLPH3 protein associates with Sqh and directly binds the centralspindlin subunit Pavarotti. We propose that during cytokinesis, the reciprocal dependence between Myosin and PI(4)P–GOLPH3 regulates centralspindlin stabilization at the invaginating plasma membrane and contractile ring assembly.

Footnotes

  • Competing interests

    The authors declare no competing or financial interests.

  • Author contributions

    Conceptualization: S.S., A.F., R.F., M.G.G.; Methodology: S.S., A.F., R.F., M.G.G.; Validation: S.S., A.F., R.F., M.G.G.; Formal analysis: A.F., A.K.-G., R.F., M.G.G.; Investigation: S.S., A.F., A.K.-G., R.F., M.G.G.; Writing - original draft: M.G.G.; Writing - review & editing: M.G.G.; Visualization: S.S., A.F., A.K.-G., R.F., M.G.G.; Supervision: M.G.G.; Funding acquisition: M.G.G.

  • Funding

    This work was supported by a grant from the AIRC - Fondazione AIRC per la Ricerca sul Cancro (AIRC; grant number IG2017 Id.20779) to M.G.G. A.F. was supported by a fellowship from the Fondazione Italiana per la Ricerca sul Cancro - AIRC (19686).

  • Supplementary information

    Supplementary information available online at https://jcs.biologists.org/lookup/doi/10.1242/jcs.252965.supplemental

  • Received August 13, 2020.
  • Accepted October 7, 2020.
  • © 2020. Published by The Company of Biologists Ltd
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Keywords

  • Drosophila
  • Male meiosis
  • Cytokinesis
  • Myosin
  • GOLPH3

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Research Article
A novel coordinated function of Myosin II with GOLPH3 controls centralspindlin localization during cytokinesis in Drosophila
Stefano Sechi, Anna Frappaolo, Angela Karimpour-Ghahnavieh, Roberta Fraschini, Maria Grazia Giansanti
Journal of Cell Science 2020 133: jcs252965 doi: 10.1242/jcs.252965 Published 10 November 2020
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Research Article
A novel coordinated function of Myosin II with GOLPH3 controls centralspindlin localization during cytokinesis in Drosophila
Stefano Sechi, Anna Frappaolo, Angela Karimpour-Ghahnavieh, Roberta Fraschini, Maria Grazia Giansanti
Journal of Cell Science 2020 133: jcs252965 doi: 10.1242/jcs.252965 Published 10 November 2020

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