Handling Editor: David Glover
ABSTRACT
In animal cell cytokinesis, interaction of non-muscle myosin II (NMII) with F-actin provides the dominant force for pinching the mother cell into two daughters. Here we demonstrate that celibe (cbe) is a missense allele of zipper, which encodes the Drosophila Myosin heavy chain. Mutation of cbe impairs binding of Zipper protein to the regulatory light chain Spaghetti squash (Sqh). In dividing spermatocytes from cbe males, Sqh fails to concentrate at the equatorial cortex, resulting in thin actomyosin rings that are unable to constrict. We show that cbe mutation impairs localization of the phosphatidylinositol 4-phosphate [PI(4)P]-binding protein Golgi phosphoprotein 3 (GOLPH3, also known as Sauron) and maintenance of centralspindlin at the cell equator of telophase cells. Our results further demonstrate that GOLPH3 protein associates with Sqh and directly binds the centralspindlin subunit Pavarotti. We propose that during cytokinesis, the reciprocal dependence between Myosin and PI(4)P–GOLPH3 regulates centralspindlin stabilization at the invaginating plasma membrane and contractile ring assembly.
Footnotes
Competing interests
The authors declare no competing or financial interests.
Author contributions
Conceptualization: S.S., A.F., R.F., M.G.G.; Methodology: S.S., A.F., R.F., M.G.G.; Validation: S.S., A.F., R.F., M.G.G.; Formal analysis: A.F., A.K.-G., R.F., M.G.G.; Investigation: S.S., A.F., A.K.-G., R.F., M.G.G.; Writing - original draft: M.G.G.; Writing - review & editing: M.G.G.; Visualization: S.S., A.F., A.K.-G., R.F., M.G.G.; Supervision: M.G.G.; Funding acquisition: M.G.G.
Funding
This work was supported by a grant from the AIRC - Fondazione AIRC per la Ricerca sul Cancro (AIRC; grant number IG2017 Id.20779) to M.G.G. A.F. was supported by a fellowship from the Fondazione Italiana per la Ricerca sul Cancro - AIRC (19686).
Supplementary information
Supplementary information available online at https://jcs.biologists.org/lookup/doi/10.1242/jcs.252965.supplemental
- Received August 13, 2020.
- Accepted October 7, 2020.
- © 2020. Published by The Company of Biologists Ltd
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