Summary
The membranes of kidney cells of 3-spined sticklebacks were examined in freeze-etch replicas. The numbers of particles adhering to surfaces and fracture faces of the outer cell membranes and the membranes of the basal labyrinth were determined. The latter membranes probably are the main location of ion-transporting enzyme complexes.
The total number of particles per cell in freshwater fish exceeds that of seawater fish by about 50 % for the outer cell membrane, and by almost 200 % for the membranes of the basal labyrinth.
After transfer of seawater fish to freshwater, particle numbers increase and their densities approximate freshwater values after 20 h. This rise in particle numbers coincides with the increase of ion-transporting activity of the cells known to take place after transfer to freshwater.
The rate of increase of particle densities is enhanced after injection of ovine prolactin. This hormone is known to stimulate Na+/K+-ATPase activity of the basal labyrinth of teleost kidney cells.
The results indicate that the particles represent enzyme complexes. The number of particles is probably under hormonal control. The increase in particle densities after transfer to freshwater is accompanied by a rise in the number of nuclear pores, which is noticeable by 10 h.
No changes were observed in the density of the particles adhering to the fracture faces of gap junctions.
- Received March 15, 1974.
- Copyright © 1974 The Company of Biologists Ltd.
Article navigation
Related articles
Cited by...
More in this TOC section
Similar articles
Other journals from The Company of Biologists
2020 at The Company of Biologists
Despite the challenges of 2020, we were able to bring a number of long-term projects and new ventures to fruition. While we look forward to a new year, join us as we reflect on the triumphs of the last 12 months.
Mole – The Corona Files
"This is not going to go away, 'like a miracle.' We have to do magic. And I know we can."
Mole continues to offer his wise words to researchers on how to manage during the COVID-19 pandemic.
Cell scientist to watch – Christine Faulkner
In an interview, Christine Faulkner talks about where her interest in plant science began, how she found the transition between Australia and the UK, and shares her thoughts on virtual conferences.
Read & Publish participation extends worldwide
“The clear advantages are rapid and efficient exposure and easy access to my article around the world. I believe it is great to have this publishing option in fast-growing fields in biomedical research.”
Dr Jaceques Behmoaras (Imperial College London) shares his experience of publishing Open Access as part of our growing Read & Publish initiative. We now have over 60 institutions in 12 countries taking part – find out more and view our full list of participating institutions.
JCS and COVID-19
For more information on measures Journal of Cell Science is taking to support the community during the COVID-19 pandemic, please see here.
If you have any questions or concerns, please do not hestiate to contact the Editorial Office.