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Research Article
Suppression of microtubule assembly kinetics by the mitotic protein TPX2
Taylor A. Reid, Breanna M. Schuster, Barbara J. Mann, Sai Keshavan Balchand, Melissa Plooster, Mark McClellan, Courtney E. Coombes, Pat Wadsworth, Melissa K. Gardner
Journal of Cell Science 2016 : jcs.178806 doi: 10.1242/jcs.178806 Published 11 February 2016
Taylor A. Reid
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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Breanna M. Schuster
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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Barbara J. Mann
2Department of Biology, University of Massachusetts, Amherst, MA, United States, 01003
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Sai Keshavan Balchand
2Department of Biology, University of Massachusetts, Amherst, MA, United States, 01003
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Melissa Plooster
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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Mark McClellan
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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Courtney E. Coombes
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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Pat Wadsworth
2Department of Biology, University of Massachusetts, Amherst, MA, United States, 01003
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  • For correspondence: klei0091@umn.edu patw@bio.umass.edu
Melissa K. Gardner
1Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN, United States, 55455
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  • For correspondence: klei0091@umn.edu patw@bio.umass.edu
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Abstract

TPX2 is a widely conserved microtubule-associated protein that is required for mitotic spindle formation and function. Previous studies have demonstrated that TPX2 is required for the nucleation of microtubules around chromosomes, however, the molecular mechanism by which TPX2 promotes microtubule nucleation remains a mystery. In this study, we found that TPX2 acts to suppress tubulin subunit off-rates during microtubule assembly and disassembly, thus allowing for the support of unprecedentedly slow rates of plus-end microtubule growth, and also leading to a dramatically reduced microtubule shortening rate. These changes in microtubule dynamics can be explained in computational simulations by a moderate increase in tubulin-tubulin bond strength upon TPX2 association with the microtubule lattice, which in turn acts to reduce the departure rate of tubulin subunits from the microtubule ends. Thus, the direct suppression of tubulin subunit off-rates by TPX2 during microtubule growth and shortening could provide a molecular mechanism to explain the nucleation of new microtubules in the presence of TPX2.

  • Received August 15, 2015.
  • Accepted February 5, 2016.
  • © 2016. Published by The Company of Biologists Ltd
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Research Article
Suppression of microtubule assembly kinetics by the mitotic protein TPX2
Taylor A. Reid, Breanna M. Schuster, Barbara J. Mann, Sai Keshavan Balchand, Melissa Plooster, Mark McClellan, Courtney E. Coombes, Pat Wadsworth, Melissa K. Gardner
Journal of Cell Science 2016 : jcs.178806 doi: 10.1242/jcs.178806 Published 11 February 2016
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Research Article
Suppression of microtubule assembly kinetics by the mitotic protein TPX2
Taylor A. Reid, Breanna M. Schuster, Barbara J. Mann, Sai Keshavan Balchand, Melissa Plooster, Mark McClellan, Courtney E. Coombes, Pat Wadsworth, Melissa K. Gardner
Journal of Cell Science 2016 : jcs.178806 doi: 10.1242/jcs.178806 Published 11 February 2016

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