Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact the journal
    • Subscriptions
    • Advertising
    • Feedback
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

User menu

  • Log in

Search

  • Advanced search
Journal of Cell Science
  • COB
    • About The Company of Biologists
    • Development
    • Journal of Cell Science
    • Journal of Experimental Biology
    • Disease Models & Mechanisms
    • Biology Open

supporting biologistsinspiring biology

Journal of Cell Science

  • Log in
Advanced search

RSS   Twitter  Facebook   YouTube  

  • Home
  • Articles
    • Accepted manuscripts
    • Issue in progress
    • Latest complete issue
    • Issue archive
    • Archive by article type
    • Special issues
    • Subject collections
    • Interviews
    • Sign up for alerts
  • About us
    • About JCS
    • Editors and Board
    • Editor biographies
    • Travelling Fellowships
    • Grants and funding
    • Journal Meetings
    • Workshops
    • The Company of Biologists
    • Journal news
  • For authors
    • Submit a manuscript
    • Aims and scope
    • Presubmission enquiries
    • Fast-track manuscripts
    • Article types
    • Manuscript preparation
    • Cover suggestions
    • Editorial process
    • Promoting your paper
    • Open Access
    • JCS Prize
    • Manuscript transfer network
    • Biology Open transfer
  • Journal info
    • Journal policies
    • Rights and permissions
    • Media policies
    • Reviewer guide
    • Sign up for alerts
  • Contact
    • Contact the journal
    • Subscriptions
    • Advertising
    • Feedback
Accepted Manuscript
Research Article
Loss of CAMSAP3 promotes EMT via the modification of microtubule-Akt machinery
Varisa Pongrakhananon, Onsurang Wattanathamsan, Masatoshi Takeichi, Paninee Chetprayoon, Pithi Chanvorachote
Journal of Cell Science 2018 : jcs.216168 doi: 10.1242/jcs.216168 Published 3 October 2018
Varisa Pongrakhananon
Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Varisa Pongrakhananon
  • For correspondence: varisa.p@pharm.chula.ac.th
Onsurang Wattanathamsan
Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, ThailandInter-department Program of Pharmacology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masatoshi Takeichi
Laboratory for Cell adhesion and Tissue Patterning, RIKEN Center for Developmental Biology and RIKEN Center for Biosystems Dynamics Research, Kobe 650-0047, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Paninee Chetprayoon
Nano Safety and Risk Assessment Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Pathum Thani 12120, Thailand
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pithi Chanvorachote
Cell-Based Drug and Health Product Development Research Unit, Chulalongkorn University, Bangkok 10330, ThailandDepartment of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF
Loading

Abstract

Epithelial-to-mesenchymal transition (EMT) plays pivotal roles in a variety of biological processes, including cancer invasion. EMT involves alterations of cytoskeletal proteins, including microtubules. The role of microtubules in EMT, however, is not fully understood. Microtubule dynamics are regulated by microtubule-binding proteins, and one such protein is CAMSAP3, which binds the minus-end of microtubules. Here, we show that CAMSAP3 is important to preserve the epithelial phenotypes in lung carcinoma cells. Deletion of CAMSAP3 in human lung carcinoma-derived cell lines showed that CAMSAP3-deficient cells acquired increased mesenchymal features mostly at transcriptional levels. Analysis of the mechanisms underlying these changes showed that tubulin acetylation was dramatically increased following CAMSAP3 removal, leading to the upregulation of protein kinase B/Akt activity, which is known to promote EMT. These findings suggested that CAMSAP3 functions to protect lung carcinoma cells against EMT by suppressing Akt activity via microtubule regulation and that CAMSAP3 loss promotes EMT in these cells.

  • Received January 30, 2018.
  • Accepted September 23, 2018.
  • © 2018. Published by The Company of Biologists Ltd
http://www.biologists.com/user-licence-1-1/
Previous ArticleNext Article
Back to top
Previous ArticleNext Article

Latest complete issue

Keywords

  • Calmodulin-regulated spectrin-associated protein 3 (CAMSAP3)
  • Cell migration
  • Epithelial-to-mesenchymal transition (EMT)
  • Protein kinase B (Akt)
  • Tubulin acetylation

 Download PDF

Email

Thank you for your interest in spreading the word on Journal of Cell Science.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Loss of CAMSAP3 promotes EMT via the modification of microtubule-Akt machinery
(Your Name) has sent you a message from Journal of Cell Science
(Your Name) thought you would like to see the Journal of Cell Science web site.
Share
Accepted Manuscript
Research Article
Loss of CAMSAP3 promotes EMT via the modification of microtubule-Akt machinery
Varisa Pongrakhananon, Onsurang Wattanathamsan, Masatoshi Takeichi, Paninee Chetprayoon, Pithi Chanvorachote
Journal of Cell Science 2018 : jcs.216168 doi: 10.1242/jcs.216168 Published 3 October 2018
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Accepted Manuscript
Research Article
Loss of CAMSAP3 promotes EMT via the modification of microtubule-Akt machinery
Varisa Pongrakhananon, Onsurang Wattanathamsan, Masatoshi Takeichi, Paninee Chetprayoon, Pithi Chanvorachote
Journal of Cell Science 2018 : jcs.216168 doi: 10.1242/jcs.216168 Published 3 October 2018

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Alerts

Please log in to add an alert for this article.

Sign in to email alerts with your email address

Article navigation

  • Top
  • Article
  • Figures & tables
  • Supp info
  • Info & metrics
  • PDF + SI
  • PDF

Related articles

Cited by...

More in this TOC section

  • Epidermal growth factor receptor signaling suppresses αvβ6 integrin and promotes periodontal inflammation and bone loss
  • IL-15Rα membrane anchorage in either cis or trans is required for stabilization of IL-15 and optimal signaling
  • A novel interplay between GEFs orchestrates Cdc42 activity during cell polarity and cytokinesis in fission yeast
Show more RESEARCH ARTICLE

Similar articles

Other journals from The Company of Biologists

Development

Journal of Experimental Biology

Disease Models & Mechanisms

Biology Open

Advertisement

First Person interviews

Photo collage of Chen Yu, Sushmita Chatterjee, Ruiqi Wang and Lotte Vanheer.

Have you seen our First Person interviews with the early-career first authors of our papers? The authors talk about their work in and out of the lab, the journeys that led them to where they are now and the scientists who inspired them along the way. Recently, we caught up with first authors Maitreyi Rathod, Sushmita Chatterjee, Lotte Vanheer, Rachel Furlong, Pamela Adami, Ruiqi Wang and Chen Yu.


Travelling Fellowship – New imaging approach unveils a bigger picture

Highlights from Travelling Fellowship trips

Find out how Pamela Imperadore’s Travelling Fellowship grant from The Company of Biologists took her to Germany, where she used new imaging techniques to investigate the cellular machinery underlying octopus arm regeneration. Don’t miss the next application deadline for 2020 travel, coming up on 29 November. Where will your research take you?


preLights – Meet the preLighters: an interview with Maiko Kitaoka

Maiko Kitaoka

Maiko Kitaoka is a graduate student in the lab of Rebecca Heald at the University of California, Berkeley. Here she studies the cause of chromosome mis-segregation defects in Xenopus hybrids. We caught up with Maiko to discuss her research, science communication, ballet, preprints and more.


Journal Meeting – Cell Dynamics: Host-Pathogen Interface

Registration is now open for the third instalment of the highly successful Cellular Dynamics Meeting Series, and will focus on ‘Host-Pathogen Interface’. The meeting will take place 17-20 May 2020, and further information is available here.


Cell Science at a Glance – Adaptor protein complexes and disease at a glance

A new poster from the Robinson lab summarises what is known about the five adaptor protein complexes and discuss how this helps to explain the clinical features of different genetic disorders.


JCS joins the Review Commons initiative

Journal of Cell Science is pleased to be a part of the new and exciting Review Commons initiative, launched by EMBO and ASAPbio. Streamlining the publishing process, Review Commons enables high-quality peer review to take place before journal submission. Papers submitted to Review Commons will be assessed independently of any journal, focusing solely on the paper’s scientific rigor and merit.


Articles of interest in our sister journals

Casein kinase 1α decreases β-catenin levels at adherens junctions to facilitate wound closure in Drosophila larvae
Chang-Ru Tsai, Michael J. Galko
Development

Spherical spindle shape promotes perpendicular cortical orientation by preventing isometric cortical pulling on both spindle poles during C. elegans female meiosis
Elizabeth Vargas, Karen P. McNally, Daniel B. Cortes, Michelle T. Panzica, Brennan M. Danlasky, Qianyan Li, Amy Shaub Maddox, Francis J. McNally
Development

Articles

  • Accepted manuscripts
  • Issue in progress
  • Latest complete issue
  • Issue archive
  • Archive by article type
  • Special issues
  • Subject collections
  • Interviews
  • Sign up for alerts

About us

  • About Journal of Cell Science
  • Editors and Board
  • Editor biographies
  • Travelling Fellowships
  • Grants and funding
  • Journal Meetings
  • Workshops
  • The Company of Biologists

For Authors

  • Submit a manuscript
  • Aims and scope
  • Presubmission enquiries
  • Fast-track manuscripts
  • Article types
  • Manuscript preparation
  • Cover suggestions
  • Editorial process
  • Promoting your paper
  • Open Access
  • JCS Prize
  • Manuscript transfer network
  • Biology Open transfer

Journal Info

  • Journal policies
  • Rights and permissions
  • Media policies
  • Reviewer guide
  • Sign up for alerts

Contact

  • Contact Journal of Cell Science
  • Subscriptions
  • Advertising
  • Feedback

Twitter   YouTube   LinkedIn

© 2019   The Company of Biologists Ltd   Registered Charity 277992