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Accepted Manuscript
Research Article
Microtubule-independent movement of the fission yeast nucleus
Sanju Ashraf, Ye Dee Tay, David A. Kelly, Kenneth E. Sawin
Journal of Cell Science 2021 : jcs.253021 doi: 10.1242/jcs.253021 Published 23 February 2021
Sanju Ashraf
Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK
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  • ORCID record for Sanju Ashraf
  • For correspondence: ken.sawin@ed.ac.uk
Ye Dee Tay
Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK
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David A. Kelly
Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK
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Kenneth E. Sawin
Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Michael Swann Building, Max Born Crescent, Edinburgh EH9 3BF, UK
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Abstract

Movement of the cell nucleus typically involves the cytoskeleton and either polymerization-based pushing forces or motor-based pulling forces. In fission yeast Schizosaccharomyces pombe, nuclear movement and positioning are thought to depend on microtubule polymerization-based pushing forces. Here we describe a novel, microtubule-independent, form of nuclear movement in fission yeast. Microtubule-independent nuclear movement is directed towards growing cell tips, and it is strongest when the nucleus is close to a growing cell tip, and weakest when the nucleus is far from that tip. Microtubule-independent nuclear movement requires actin cables but does not depend on actin polymerization-based pushing or myosin V-based pulling forces. Vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) Scs2 and Scs22, which are critical for endoplasmic reticulum-plasma membrane contact sites in fission yeast, are also required for microtubule-independent nuclear movement. We also find that in cells in which microtubule-based pushing forces are present, disruption of actin cables leads to increased fluctuations in interphase nuclear positioning and subsequent altered septation. Our results suggest two non-exclusive mechanisms for microtubule-independent nuclear movement, which may help illuminate aspects of nuclear positioning in other cells.

  • Received August 17, 2020.
  • Accepted February 9, 2021.
  • © 2021. Published by The Company of Biologists Ltd
http://creativecommons.org/licenses/by/4.0

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Keywords

  • Fission yeast
  • Schizosaccharomyces pombe
  • Nucleus
  • Nuclear movement
  • Actin
  • Endoplasmic reticulum

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Accepted Manuscript
Research Article
Microtubule-independent movement of the fission yeast nucleus
Sanju Ashraf, Ye Dee Tay, David A. Kelly, Kenneth E. Sawin
Journal of Cell Science 2021 : jcs.253021 doi: 10.1242/jcs.253021 Published 23 February 2021
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Accepted Manuscript
Research Article
Microtubule-independent movement of the fission yeast nucleus
Sanju Ashraf, Ye Dee Tay, David A. Kelly, Kenneth E. Sawin
Journal of Cell Science 2021 : jcs.253021 doi: 10.1242/jcs.253021 Published 23 February 2021

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