Autophagy and microtubules – new story, old players

Autophagy is a catabolic process that leads to the degradation of cytoplasmic components by lysosomes. There are several types of autophagy, such as macroautophagy, microautophagy and chaperone-mediated autophagy (CMA) (for a review, see Mizushima and Komatsu, 2011). Here, we will focus on macroautophagy (hereafter referred to as autophagy). The initial step of autophagy is the formation of a cup-shaped structure, named the phagophore (Fig. 1A). In most cell types, autophagy proceeds at a basal rate to ensure a level of quality control of the cytoplasm and to maintain cellular homeostasis, by eliminating long-lived or aggregated proteins and damaged organelles, including mitochondria (for a review, see Yang and Klionsky, 2010). However, under several stress conditions (e.g. metabolic stress, hypoxia, chemotherapy), autophagy is greatly induced to cope with the stress and to fulfil the energetic or biosynthetic demand, thereby allowing cell survival (for reviews, see Kroemer et al., 2010; Singh and Cuervo, 2011; Yang and Klionsky, 2010). Autophagy not only has an important physiological role in stress management but is also involved in development and immunity (reviewed by Deretic and Levine, 2009; Levine and Klionsky, 2004). Deregulation of autophagy leads to a wide range of disorders, such as cancers, neurodegenerative disorders, heart and liver diseases, myopathies and ageing (Ravikumar et al., 2010; Rubinsztein et al., 2011).

The autophagosome membrane elongates to surround the cytoplasmic material to be degraded (e.g. proteins, lipid droplets, organelles and pathogens), before it expands, curves and then closes to form a double-membrane vesicle. The sequestered material is then degraded by acid hydrolases that reside in the lysosome. The resulting degradation products (e.g. amino acids, fatty acids and nucleotides) are exported through membrane permeases to the cytoplasm, where they are recycled for the biosynthesis of new macromolecules or the production of ATP (Mehrpour et al., 2010; Yang and Klionsky, 2010).

Similar to most of the membrane-bound organelles and vesicles, various aspects of autophagosome dynamics rely in part on their interactions with the cytoskeleton and especially with microtubules (MTs) (Monastyrska et al., 2009). Although the involvement of MTs in many steps of autophagosome dynamics has been a matter of considerable debate over the past years, it appears that a consistent picture is now emerging in which MTs have essential roles in coordinating and organizing many crucial steps of autophagosome life. After providing a brief overview of autophagy and of microtubule function and dynamics, we will focus here on the roles that MTs and microtubule molecular motors have in autophagosome formation, their movements and their interaction with lysosomes. We will also discuss recent advances that highlight the importance of global organelle positioning and of MT acetylation in autophagy.

More than 30 autophagy-related gene products (ATGs) are dedicated to the execution of autophagy (for reviews, see Mizushima et al., 2011; Yang and Klionsky, 2010). Most of them have been identified by genetic screens in yeast and are evolutionarily conserved in mammals. The core machinery of autophagy includes the following steps: (i) initiation of autophagy and vesicle nucleation; (ii) vesicle expansion and closure; (iii) autophagosome maturation and its fusion with the late endosome, and degradation (Fig. 1A). Initiation of autophagy starts with the activation of the ULK1 complex (Atg1 complex in budding yeast) that contains the serine/threonine-protein kinase ULK1, ATG13, focal adhesion kinase family kinase-interacting protein of 200 kD (FIP200, also known as RBCC1) and ATG101 (reviewed by Mizushima, 2010). Upon induction of autophagy, this complex localizes to the site of phagophore formation to regulate the nucleation machinery. Phagophore nucleation is highly dependent on the production of phosphatidylinositol 3-phosphate [PtdIns(3)P (PI3P)] by a phosphoinositide 3-kinase (PI 3-kinase) complex that is recruited to the phagophore membrane during the initiation step. This complex comprises the class III PI 3-kinase Vps34 (PK3C3), Beclin 1 (the mammalian ortholog of Atg6 in yeast), ATG14, Vps15 and activating molecule in BECN1-regulated autophagy protein 1 (AMBRA1) (Funderburk et al., 2010). The production of PtdIns(3)P in the phagophore membrane allows the recruitment of the WD repeat domain phosphoinositide-interacting proteins WIPI1 and WIPI2 (the mammalian orthologs of Atg18 in budding yeast) (Polson et al., 2010). Both contribute to the expansion and the closure of the vesicle in concert with two ubiquitin-like conjugation systems, resulting in the ATG12–ATG5-ATG16L complex and the phosphatidylethanolamine (PtdEtn) conjugate of microtubule-associated protein 1 light chain 3 [LC3 (MLP3); the mammalian ortholog of Atg8 in budding yeast] (Fujita et al., 2008; Hanada et al., 2007; for a review, see Xie and Klionsky, 2007). The transmembrane protein ATG9 also participates in the nucleation and the expansion of the phagophore membrane by cycling between different compartments and the phagophore (Orsi et al., 2012) (Fig. 1C). Autophagosome maturation and its fusion with the lysosome occur in the vicinity of the centrosome and depend on several lysosomal membrane proteins, such as the small GTPase Ras-related protein Rab7 and the transmembrane lysosome-associated membrane glycoprotein 2 (LAMP2) (Gutierrez et al., 2004; Jäger et al., 2004; Tanaka et al., 2000). Degradation of autophagosomal cargoes is then achieved by the acid hydrolases and the cathepsin proteases that are present in the lysosomal lumen.

Induction of autophagy is strictly regulated through upstream signaling pathways that are governed by growth factors, amino acids, glucose and the energy status (Fig. 1B). Most of these pathways control the two initiation complexes ULK1 and PI 3-kinase . Activity of the ULK1 complex is controlled by one of the main players in autophagy regulation, the mammalian target of rapamycin complex 1 (mTORC1), which comprises the mTOR serine/threonine-protein kinase and auxiliary proteins. As long as mTOR is activated, it inhibits autophagy by inhibitory phosphorylation of ULK1 (for a review, see Yang and Klionsky, 2010). Under diverse stresses including amino acid starvation, mTORC1 is inhibited, thereby promoting ULK1 complex activity and autophagy (Hosokawa et al., 2009; Jung et al., 2009). One of the important pathways that regulates mTORC1 is initiated by growth factors and involves the class I PI 3-kinase s and Akt serine/threonine-protein kinases (for a review, see Sengupta et al., 2010). Another pathway that controls autophagy in response to amino acids involves the Rag GTPase, which allows the recruitment of mTOR at the lysosomal membrane, where its direct activators reside (Sancak et al., 2010). Upon energy depletion, when the AMP:ATP ratio rises, AMP-activated protein kinase (AMPK) can also activate the ULK1 complex by directly phosphorylating ULK1 or, indirectly, by inactivating mTORC1 (Alers et al., 2012; Kim et al., 2011). Activity of the PI 3-kinase complex is also regulated, mainly through the interaction of Beclin 1 with the anti-apoptotic members of the Bcl-2 family (Kang et al., 2011). Interaction of Beclin 1 with Bcl-2 or Bcl-xL through its BH3 domain inhibits autophagy. Upon amino acid starvation, activation of c-Jun N-terminal kinase-1 [JNK1 (mitogen-activated protein kinase 8)] leads to Bcl-2 phosphorylation and the release of Beclin 1, which in turn induces autophagy (Wei et al., 2008). Interaction between Beclin 1 and apoptosis regulator Bcl-2 or Bcl-xL can also be disrupted following the phosphorylation of Beclin 1 by death-associated protein kinase (DAPK) (Zalckvar et al., 2009).

The origin of the autophagosomal membrane and the process of phagophore nucleation have long been an enigma. The phagophore appears to expand by membrane addition rather than by de novo lipid synthesis (for a review, see Yang and Klionsky, 2010). Several organelles could serve as membrane donors for autophagosome formation, such as the endoplasmic reticulum (ER), the Golgi complex, mitochondria, endosomes, the plasma membrane or the nuclear envelope (for a review, see Mizushima et al., 2011). Recent studies suggest that a specialized domain of the ER called the omegasome (Axe et al., 2008) is a privileged site for phagophore biogenesis. The autophagosomal membrane elongates inside a cradle that is formed by ER membranes, which acts as a template for the spherical shape of the autophagosome (Hayashi-Nishino et al., 2009; Ylä-Anttila et al., 2009). As we discuss below, many steps in the life cycle of autophagosomes involve MTs and their associated molecular motors.

MTs are hollow cylinders of ∼25 nm in diameter that are formed by polymerization of α–β tubulin dimers. In most mammalian cells, interphase MTs assemble at the centrosome and/or at membrane MT-organizing centers (MTOCs) such as the Golgi complex. γ-Tubulin-containing ring complexes nucleate MTs and interact with their minus-ends (for a review, see Kollman et al., 2011). MT plus-ends grow towards the cell periphery by incorporating GTP-bound tubulin subunits. Growing MTs generally hydrolyze GTP in the inner regions of the polymer, yielding a GTP–tubulin cap (David-Pfeuty et al., 1977; Dimitrov et al., 2008). Once this cap is lost at the cell periphery, MTs disassemble and release GDP–tubulin into the cytosol. The alternation between growth and shrinking phases of MTs that are separated by transitions that are termed either catastrophes (from MT assembly or pausing to disassembly) and rescues (from phases of MT shrinking or pausing to their re-growth) is called dynamic instability (Mitchison and Kirschner, 1984). Such a continuous remodelling operates independently for each MT and allows MTs to interact temporarily with cellular components, to explore the intracellular space and to position organelles dynamically (for reviews, see Desai and Mitchison, 1997; Howard, 2006). For instance, MTs maintain the ER and mitochondria that are dispersed throughout the cytoplasm, while they at the same time also maintain the Golgi complex and the endosomes that are clustered in the vicinity of the nucleus (reviewed by Cole and Lippincott-Schwartz, 1995). MTs move and position organelles by functioning as tracks, on which plus-end- (kinesins) or minus-end-directed (cytoplasmic dynein) molecular motors carry membranes, by exerting pushing and pulling forces during their assembly and depolymerization or by sliding along each other in a way that is powered by molecular motors (for a review, see Tolić-Nørrelykke, 2008). MTs could also interact directly or indirectly with a wealth of proteins that might be sequestered, released, assembled into complexes and/or transported to organize and modulate signal transduction (for a review, see Etienne-Manneville, 2010; Gundersen and Cook, 1999).

At a given time, not all the MT network of a cell is subjected to remodelling by dynamic instability. Indeed, a subpopulation comprises long-lived polymers, which can persist even for an entire interphase (so-called stable MTs) (Webster et al., 1987). MT stabilization, which is enhanced by cell confluence, might occur at the cell periphery, where MT plus-ends are capped with proteins that prevent tubulin exchange (Bartolini et al., 2008; Infante et al., 2000; Palazzo et al., 2001). This stabilization is not stochastic and can result from specific cortical interactions (reviewed by Gundersen et al., 2004) or from interactions with organelles such as the Golgi complex (Chabin-Brion et al., 2001; Efimov et al., 2007; Miller et al., 2009). In addition, stable MTs exhibit numerous tubulin post-translational modifications, such as de-tyrosination, polyglutamylation or acetylation (for a review, see Janke and Bulinski, 2011). These modifications might prevent the binding of disassembling factors or modulate MT functions by fine-tuning the binding of proteins to the MT surface. Stable and dynamic MT subpopulations are functionally specialized in organizing signaling pathways or vesicle trafficking. For example, dynamic MTs are involved in basolateral-directed post-Golgi trafficking in epithelial cells, as well as in transcytosis (Hunziker et al., 1990; Lafont et al., 1994; Poüs et al., 1998) or in caveolae-mediated pathogen internalization (Guignot et al., 2001). Stable MTs participate in ER-to-Golgi traffic (Mizuno and Singer, 1994), the Golgi-to-plasma-membrane traffic (Cai et al., 2009) or in the recycling of endosomes to the plasma membrane (Lin et al., 2002). Stable MTs are also involved in alcohol-induced alterations in protein traffic in hepatocytes (Joseph et al., 2008). Vesicular carriers are thought to recognize the MT tracks they require through interaction with molecular motors, especially with kinesins (Cai et al., 2009). Indeed, the kinesin-1 KIF5C preferentially moves on stable and post-translationally modified MTs, whereas the kinesin-2 KIF17 and the kinesin-3 KIF1A are not selective (Cai et al., 2009).

Various pharmacological MT-targeting agents (anti-polymerization drugs, such as nocodazole or vinca alkaloids, and MT-stabilizing drugs such as Taxol) and biochemical procedures are used to identify the role the MT network has in biological processes, including the specific involvement of dynamic and stable MT subpopulations (see Box 1). An indirect relationship between macroautophagy and MTs was first proposed over 35 years ago (Amenta et al., 1977). This study reported that the vinca alkaloids vincristine or vinblastine inhibit the autophagic protein degradation occurring in response to serum deprivation. Such a global response includes: first, the formation of autophagosomes and, second, their fusion with lysosomes and subsequent protein degradation. The following sections address the roles of MTs and of their associated molecular motors in these two steps of autophagy.

The role of MTs in autophagosome formation appears to be different between basal and stress-induced autophagy. Under basal conditions, several studies using nocodazole and Taxol suggest that MTs do not participate in autophagosome formation (Aplin et al., 1992; Köchl et al., 2006; Reunanen et al., 1988). Upon amino acid starvation, disassembling MTs with high doses of nocodazole prevents autophagosome formation, highlighting the role of MTs in this step (Geeraert et al., 2010; Köchl et al., 2006). In addition, MT stabilization by Taxol or by submicromolar nocodazole concentrations has the same effect, suggesting that MT dynamics are also important (Geeraert et al., 2010; Köchl et al., 2006). In the above studies, the functional importance of MTs in autophagosome formation essentially relies on the use of tubulin-acting drugs and thus might be subject to misinterpretations owing to possible side effects. This is especially the case with the experiments performed with vinca alkaloids, in which autophagosome formation is enhanced both under basal or amino acid starvation conditions (Köchl et al., 2006). This puzzling behaviour might result from the fact that vinca alkaloids cause tubulin precipitation into paracrystals. These structures, even if they do not resemble functional MTs, could perhaps act as molecular scaffolds that facilitate autophagosome formation.

The hypothesis of MT involvement in autophagosome formation has been reinforced and refined by complementary findings. First, both the non-lipidated and the PtdEtn-conjugated forms of LC3 are found in MT-containing subcellular fractions, suggesting that an interaction occurs between LC3 and MTs (Fass et al., 2006; Geeraert et al., 2010). LC3 has been shown to associate with MTs indirectly through an interaction with microtubule-associated proteins MAP1A and MAP1B (Mann and Hammarback, 1994) or its homolog MAP1S (Xie et al., 2011). LC3 might also interact directly with tubulin through its N-terminal domain (Kouno et al., 2005). Moreover, several other ATG proteins (ULK1, Beclin 1, WIPI1, ATG5, ATG12) that are involved in the early steps of autophagosome formation are enriched in the dynamic MT fraction (Table 1), suggesting that the dynamic subset of MTs supports the assembly of pre-autophagosomal structures. Unlike phagophores, which are immobile (Fass et al., 2006; Kimura et al., 2008), WIPI1-positive pre-autophagosomal structures move along MTs upon starvation of the cell (Geeraert et al., 2010). Interestingly, such movements are highly sensitive to nocodazole treatment under conditions that affect only highly dynamic MTs. This raises the possibility that motors associated with these MTs could drive other important steps of autophagosome formation such as the homotypic fusion of ATG16-positive membranes, which then maturate into phagophores (Moreau et al., 2011). Second, it has been shown that MTs and MT motors regulate two major complexes involved in the initiation of the autophagic response: mTORC1 and the class III PI 3-kinase complex. mTOR binds to the cytoplasmic face of lysosomes (Sancak et al., 2010), and its activity is controlled by lysosome localization. This localization is organized by MTs and involves two kinesins: the kinesin-2 KIF2A and the kinesin-3 KIF1B (Korolchuk et al., 2011). When nutrients are available, these kinesins localize to lysosomes at the cell periphery, and mTORC1 is kept active, thereby preventing autophagosome formation. When nutrients are in short supply, the cytoplasmic pH increases, these motors are released from lysosomes, facilitating their centripetal movement, mTORC1 inactivation and autophagosome formation (Fig. 2A) (Korolchuk et al., 2011; Poüs and Codogno, 2011). Beclin 1 is a major component of the class III PI 3-kinase complex. It is sequestered on MTs in two complexes containing the dynein light chain 1 (DLC1) and either AMBRA1 or Bim (Bcl2-like protein 11). Upon induction of autophagy, AMBRA1 phosphorylation by the kinase ULK1 releases Beclin 1 (Di Bartolomeo et al., 2010). In parallel, activated JNK1 phosphorylates Bim to release Beclin 1 (Luo et al., 2012), allowing it to be recruited to membranes such as omegasomes, from which autophagosomes originate (Fig. 2B). JNK1 also disrupts the ER-associated complex formed by Beclin 1 and Bcl-2 (Wei et al., 2008) (Fig. 2B). Interestingly, upon amino acid starvation, JNK1 activation is a kinesin-1-dependent process (Geeraert et al., 2010; Verhey et al., 2001), and it seems to require the hyperacetylation of the dynamic subset of MTs (Geeraert et al., 2010).

The mechanism by which tubulin acetylation (in the lumen of MTs) could modulate the recruitment and walking of molecular motors (on the outer face of MTs) is still a matter of debate and will require further investigations to determine whether it is a key actor or a more indirect phenomenon in regulating motor activity. Acetylation has been proposed to trigger changes in the lateral interaction between tubulin subunits (Cueva et al., 2012; Topalidou et al., 2012). A consecutive change in MT conformation might therefore enhance motor recruitment to MTs (Dompierre et al., 2007; Hammond et al., 2009; Reed et al., 2006). Conversely, the role of tubulin acetylation upon molecular motor recruitment and function has been questioned in motility assays using tubulin acetylated in vitro (Soppina et al., 2012; Walter et al., 2012). These studies proposed that the recruitment of kinesin-1 to acetylated MTs and its velocity were not affected, but other tubulin modifications (de-tyrosination and polyglutamylation) could have masked the effect of acetylation. The level of tubulin acetylation depends on the histone deacetylase HDAC6 and the NAD-dependent protein deacetylase sirtuin-2 (SIRT2) (Hubbert et al., 2002; North et al., 2003; Zhang et al., 2003), which have also been shown to regulate autophagy. SIRT2 inhibits this process (Zhao et al., 2010), whereas HDAC6 functions as a scaffold that binds to polyubiquitylated proteins to allow the formation of aggresomes and binds to damaged mitochondria that are cleared by autophagy (Kawaguchi et al., 2003; Lee et al., 2010; Pandey et al., 2007). The relationship between MTs and the function of SIRT2 in autophagy is not straightforward given its inhibitory activity, but HDAC6 sequestration in cytoplasmic regions engulfed by autophagosomes could perhaps contribute to its inactivation and thus to the induction of tubulin acetylation. Whatever the exact roles deacetylases have in autophagosome formation, the functional importance of tubulin acetylation will deserve further clarification.

Altogether, the above data support the idea that stress-induced autophagosome formation involves MTs, and many proteins involved in this process localize on the dynamic MT subset. Interestingly, the rate of autophagosome formation (∼1/minute) (Fass et al., 2006) or the lifetime of omegasomes (<3 minutes) (Ktistakis et al., 2011) is consistent with the life span of dynamic MTs (a few minutes). By contrast, mature autophagosomes exist for ∼30 minutes before they fuse with lysosomes (Fass et al., 2006; Ktistakis et al., 2011). This period fits well with the life span of stable MTs, along which they could get clustered near the nucleus and meet lysosomes, as discussed in the next section.

The role of MTs in the fusion of autophagosomes with lysosomes has long been controversial, as most studies did not clearly distinguish transport from fusion. The involvement of MTs in autolysosome formation was initially proposed based on the observation that a complete disassembly of MTs inhibits the colocalization of autophagosomes and lysosomes and/or protein degradation by autophagy (Aplin et al., 1992; Köchl et al., 2006; Webb et al., 2004). The role of MTs in autolysosome formation is likely to depend on stable MTs. Indeed, Taxol-mediated MT stabilization does not affect autophagosome and lysosome fusion, suggesting that MT dynamics are not involved in the gathering of autophagosomes and lysosomes or in their fusion (Köchl et al., 2006). Also, under basal conditions, stable acetylated MTs seem to participate in autolysosome formation (Xie et al., 2010). Fass and colleagues precisely determined MT participation in autophagosome-to-lysosome fusion events in CHO cells starved of amino acids and confirmed that protein degradation is impaired by MT disassembly. They also found that the lifetime of autophagosomes does not change in the absence of MTs (Fass et al., 2006). The authors proposed that fusion would still occur in the absence of MTs, but that nocodazole impairs lysosomal degradation owing to a drop in protease transport to lysosomes, as shown in the early 1990s (Fass et al., 2006; Scheel et al., 1990). Taken together, these results suggest that MTs are dispensable for the fusion between autophagosomes and lysosomes. However, a trafficking of autophagosomes along MTs towards lysosomes is necessary to allow effective fusion, as has been shown by Kimura and colleagues (Kimura et al., 2008) using fluorescence recovery after photobleaching (FRAP) assays.

Once they have formed, autophagosomes move bi-directionally along MTs and finally concentrate around the centrosome in the perinuclear region. The centripetal movement of autophagosomes is mediated by the MT minus-end-directed motor dynein, as shown using an inhibitor of dynein ATPase activity (Jahreiss et al., 2008). Furthermore, dominant-negative mutations of the gene encoding dynein were shown to decrease the autophagy-mediated clearance of a mutant form of α-synuclein, which causes familial Parkinson disease (Ravikumar et al., 2005). In this study, the expression of dynein mutants also increased the number of autophagosomes and impaired their fusion with lysosomes. Dynein involvement was confirmed by a decrease in autophagosome movements in HeLa cells that expressed GFP–LC3 and p50 dynamitin, which disrupts the dynein–dynactin complex and impairs centripetal organelle movements (Ravikumar et al., 2005). A similar reduction was observed after blocking LC3 function by microinjecting a specific antibody during amino acid starvation (Kimura et al., 2008). LC3 might participate in the recruitment of dynein to autophagosomes. Indeed, LC3 can bind to Rab7 (Pankiv et al., 2010), which has been shown to be involved in the recruitment of the dynein-associated dynactin subunit 1 protein (p150^Glued) (Johansson et al., 2007) and in autophagosome maturation (Gutierrez et al., 2004; Jäger et al., 2004). Regarding the centrifugal movement of autophagosomes, kinesin-1 is involved under basal conditions (Cardoso et al., 2009; Geeraert et al., 2009), but the kinesin motor(s) involved in stress conditions are still unknown. A possible functional link between autophagosomes and kinesins in stressed cells might involve FYVE and coiled-coil domain-containing protein 1 (FYCO1), which contains a possible kinesin binding site and is recruited to autophagosomes by means of Rab7 and LC3 (Pankiv et al., 2010) (Fig. 2A).

Altogether, the importance of MTs in the formation of autolysosomes mainly relies on their role in the localization of autophagosomes and lysosomes in the juxta-nuclear region. This gathering most likely results from their ‘motorization’ by dynein after inhibition of centrifugal trafficking that is mediated by kinesins. The emerging picture thus shows that the spatial control of autophagosome location during their formation and movements depends both on their compartmentalization between dynamic and stable MTs and on their subcellular positioning through the balance between dynein and kinesin activities. These mechanisms might thus prevent the premature fusion of immature autophagosomes with lysosomes.

It is now clear that MTs are involved in different steps of autophagy – that is, in the formation and motility of autophagosomes, but not in their fusion with lysosomes. MTs might serve as local suppliers or as scaffolds to promote the interaction of proteins that are required during the early stages of autophagy, such as Beclin 1 or AMBRA1. In addition, numerous studies have demonstrated that MTs have a role in the motility of autophagosomes. Here, we have discussed how two characteristics of MTs are also important for autophagosome formation: the dynamics of MTs and the post-translational modification of tubulin, in particular its acetylation. This adds a novel layer to the growing importance of acetylation in the overall regulation of autophagy (Hamaï and Codogno, 2012). MT dynamics also provide cells with a way to compartmentalize and organize, first, the upstream signaling of autophagosome formation and, second, autophagosome biogenesis and trafficking. The relationship between MTs and autophagy thus expands the repertoire of MT function in terms of signaling and membrane trafficking.

Finally, the role of MTs in autophagy needs to be considered from a therapeutic point of view. MT-targeting agents are being used in tumours as they inhibit mitosis. However, human tumour cells divide more slowly than cultured cell lines and tumour cells in animal models (for a review, see Komlodi-Pasztor et al., 2011). As MTs have a main role in intracellular trafficking, signaling and autophagy in non-mitotic cells, these processes might also constitute effective targets in cancer cells that are treated with MT-targeting agents. Indeed, at least part of the cytotoxic effect of the MT-stabilizer Taxol is dependent on its ability to block autophagosome maturation (Veldhoen et al., 2012). Conversely, other tubulin-binding drugs, such as vinblastine, naphtharazin or 2-methoxyestradiol, might instead stimulate autophagy, which could contribute to their anticancer activity (Acharya et al., 2011; Chen et al., 2008; Kamath et al., 2006; Köchl et al., 2006; Lorin et al., 2009). In the context of neurodegenerative diseases, the link between MT-acting drugs and autophagy could also prove to be important. For example, in hybrid cells harbouring mitochondria from Parkinson's patients, mitochondria dysfunction alters the MT network and causes an accumulation of autophagosomes and an impaired clearance of both p62 and α-synuclein (Arduíno et al., 2012). Interestingly, in these cells, Taxol improves autophagic flux, most likely because it restores MT integrity.

Regardless of the mechanisms by which MT dynamics might influence autophagy, these examples demonstrate the increasing evidence that autophagy is a ‘drugable’ process that can be exploited for the future development of human disease therapies (reviewed by Rubinsztein et al., 2012) and that MT-targeting agents should be considered as drugs that can target autophagy.