Supplementary Material

JCS062661 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 -

  Fig. S1. MUC1 promotes membrane-free EGFR nuclear localization. MDA-MB-468 cells were treated with control (Ctrl) siRNA or siRNA to knock down MUC1 (MUC1-1), and then stimulated with EGF (10 ng/ml) for 0 minutes or 120 minutes. Cells were then subjected to a high-salt nuclear protein extraction (500 mM NaCl), and protein (50 µg) was then separated by SDS-PAGE. After transfer to membrane, the proteins were analyzed by immunoblotting with antibodies against EGFR (1005), MUC1 (CT2) and Sp1 (Santa Cruz, 1C6). Molecular size markers are on the right.