Supplementary Material

JCS071134 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Table S1. Primer sequences used to generate pEntry vectors via Gateway cloning

• Supplemental Figure S1 -

  Fig. S1. Sev^S11-CFP−RFP-Drk colocalization increases at higher Sev^S11−CFP concentrations. (A−A′′′) RFP−Drk (red, A′) colocalization with Sev^S11−CFP (cyan, A). Colocalization dots can be seen in A′′′ according to a temperature-based lookup table with purple (cold) indicating low values and (hot) high values temperature. (B−B′′′) RFP−Drk (B′) colocalization with Sev^S11−CFP (B). Colocalization increases at higher Sev^S11−CFP concentration (B′′′).

• Supplemental Figure S2 -

  Fig. S2. MAPK does not interact with Sev RTK. (A) Sev^S11−CFP and RFP−MAPK scheme. (B) Sev^S11−CFP (cyan) localizes in the membrane and vesicles. (C) RFP−MAPK (red) localizes in cytoplasm and nuclei. (D−D′′′) No energy transfer was detected in cell co-transfected with both Sev^S11−CFP and RFP−MAPK. (D′′′) FRET^c intensities. Scale bars: 10 µm.