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Supplementary Material

JCS072223 Supplementary Material

Files in this Data Supplement:

  • Supplemental Table S1 -

    Table S1. Sequences of siRNA and miRNA duplexes and PCR primers.

  • Supplemental Figure S1 -

    Fig. S1. Predicted miRNAs that target ALK7. (A) miRs pairing with 3′UTR of ALK7. (B) Luciferase assays show that miR-368, but not miR-122a, miR-147, miR-412 or miR-22, significantly deceases the luciferase activity when co-transfected with a luciferase reporter construct containing ALK7 3′UTR. Data represent mean ± s.e.m. (n=4).

  • Supplemental Figure S2 -

    Fig. S2. MiR-376c does not affect luciferase activity of the p-miR-Report. To determine the specificity of miR-376c on ALK7 3′UTR, the p-miR-report plasmid lacking the 3′UTR of ALK7 was cotransfected with different concentrations of the negative control (NC) or miR-376c into both OV2008 (top panel) or A2780s cells (bottom panel). No difference between the control and miR-376c transfected cells was observed.