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Supplementary Material

JCS087718 Supplementary Material

Files in this Data Supplement:

  • Supplemental Figure S1 -

    Fig. S1. HLA I retainment. Intracellular HLA I accumulation (quantified by flow cytometry) was not observed in naïve hESC, but was induced by IB transfection. We did not find HLA I accumulation after siRNA treatment or combined siRNA + IB knockdown, which was used to create hESCKD.

  • Supplemental Figure S2 -

    Fig. S2. Teratoma growth. Tumor growth was observed in all SCID-beige mice (a) 80 days after hESC transplantation. The tumors generated high BLI signals (b). Histology confirmed that the tumors were teratomas (c) derived from all three germ layers (A: adipose tissue; B: bone; C: cartilage; D: primitive duct; En: immature entodermal epithelium; Me: primitive mesenchyme; N: immature neural tissue (neuroblasts and rosettes); Sm: smooth muscles bundles; V: blood vessels).