RT Journal Article
SR Electronic
T1 Rapid transport of neural intermediate filament protein
JF Journal of Cell Science
JO J. Cell Sci.
FD The Company of Biologists Ltd
SP 2345
OP 2359
DO 10.1242/jcs.00526
VO 116
IS 11
A1 Helfand, Brian T.
A1 Loomis, Patty
A1 Yoon, Miri
A1 Goldman, Robert D.
YR 2003
UL http://jcs.biologists.org/content/116/11/2345.abstract
AB Peripherin is a neural intermediate filament protein that is expressed in peripheral and enteric neurons, as well as in PC12 cells. A determination of the motile properties of peripherin has been undertaken in PC12 cells during different stages of neurite outgrowth. The results reveal that non-filamentous, non-membrane bound peripherin particles and short peripherin intermediate filaments, termed `squiggles', are transported at high speed throughout PC12 cell bodies, neurites and growth cones. These movements are bi-directional, and the majority require microtubules along with their associated molecular motors, conventional kinesin and cytoplasmic dynein. Our data demonstrate that peripherin particles and squiggles can move as components of a rapid transport system capable of delivering cytoskeletal subunits to the most distal regions of neurites over relatively short time periods.