PT  - JOURNAL ARTICLE
AU  - Bouquin, Thomas
AU  - Mattsson, Ole
AU  - Næsted, Henrik
AU  - Foster, Randy
AU  - Mundy, John
TI  - The <em>Arabidopsis lue1</em> mutant defines a katanin p60 ortholog
involved in hormonal control of microtubule orientation during cell
growth
AID  - 10.1242/jcs.00274
DP  - 2003 Mar 01
TA  - Journal of Cell Science
PG  - 791--801
VI  - 116
IP  - 5
4099  - http://jcs.biologists.org/content/116/5/791.short
4100  - http://jcs.biologists.org/content/116/5/791.full
SO  - J. Cell Sci.2003 Mar 01; 116
AB  - The lue1 mutant was previously isolated in a bio-imaging screen for Arabidopsis mutants exhibiting inappropriate regulation of an AtGA20ox1 promoter-luciferase reporter fusion. Here we show that lue1 is allelic to fra2, bot1 and erh3, and encodes a truncated katanin-like microtubule-severing protein (AtKSS). Complementation of lue1 with the wild-type AtKSS gene restored both wild-type stature and luciferase reporter levels. Hormonal responses of lue1 to ethylene and gibberellins revealed inappropriate cortical microtubule reorientation during cell growth. Moreover, a fusion between the AtKSS protein and GFP decorated cortical microtubules. A yeast two-hybrid screen with AtKSS as the bait identified proteins related to those involved in microtubule processing, including a katanin p80 subunit and a kinesin ortholog. These results indicate that AtKSS is involved in microtubule dynamics in response to plant hormones.