RT Journal Article
SR Electronic
T1 Dishevelled (Dvl-2) activates canonical Wnt signalling in the absence of cytoplasmic puncta
JF Journal of Cell Science
JO J. Cell Sci.
FD The Company of Biologists Ltd
SP 5279
OP 5289
DO 10.1242/jcs.02647
VO 118
IS 22
A1 Smalley, Matthew J.
A1 Signoret, Nathalie
A1 Robertson, David
A1 Tilley, Alan
A1 Hann, Anthony
A1 Ewan, Ken
A1 Ding, Yanning
A1 Paterson, Hugh
A1 Dale, Trevor C.
YR 2005
UL http://jcs.biologists.org/content/118/22/5279.abstract
AB Dishevelled family proteins are multidomain intracellular transducers of Wnt signals. Ectopically expressed mammalian Dishevelled 2 (Dvl-2) activates downstream signalling and localises to cytoplasmic puncta. It has been suggested that these Dvl-2-containing structures correspond to intracellular vesicles and may be involved in the Wnt signal transduction process. We report that cytoplasmic puncta are primarily formed in cells expressing Dvl-2 at high levels. Lower levels of expression can activate signalling without forming puncta. The structures do not localise with markers of the early or late endocytic pathway and time-lapse analysis demonstrates that Dvl-2 puncta move in a random fashion over short distances but do not originate from the plasma membrane. Based on our findings, we propose that Dvl-2 puncta are protein aggregates that are not required for signalling.