PT  - JOURNAL ARTICLE
AU  - Strádalová, Vendula
AU  - Stahlschmidt, Wiebke
AU  - Grossmann, Guido
AU  - Blažíková, Michaela
AU  - Rachel, Reinhard
AU  - Tanner, Widmar
AU  - Malinsky, Jan
TI  - Furrow-like invaginations of the yeast plasma membrane correspond to membrane compartment of Can1
AID  - 10.1242/jcs.051227
DP  - 2009 Aug 15
TA  - Journal of Cell Science
PG  - 2887--2894
VI  - 122
IP  - 16
4099  - http://jcs.biologists.org/content/122/16/2887.short
4100  - http://jcs.biologists.org/content/122/16/2887.full
SO  - J. Cell Sci.2009 Aug 15; 122
AB  - Plasma membrane of the yeast Saccharomyces cerevisiae contains stable lateral domains. We have investigated the ultrastructure of one type of domain, the membrane compartment of Can1 (MCC). In two yeast strains (nce102Δ and pil1Δ) that are defective in segregation of MCC-specific proteins, we found the plasma membrane to be devoid of the characteristic furrow-like invaginations. These are highly conserved plasma membrane structures reported in early freeze-fracture studies. Comparison of the results obtained by three different approaches – electron microscopy of freeze-etched cells, confocal microscopy of intact cells and computer simulation – shows that the number of invaginations corresponds to the number of MCC patches in the membrane of wild-type cells. In addition, neither MCC patches nor the furrow-like invaginations colocalized with the cortical ER. In mutants exhibiting elongated MCC patches, there are elongated invaginations of the appropriate size and frequency. Using various approaches of immunoelectron microscopy, the MCC protein Sur7, as well as the eisosome marker Pil1, have been detected at these invaginations. Thus, we identify the MCC patch, which is a lateral membrane domain of specific composition and function, with a specific structure in the yeast plasma membrane – the furrow-like invagination.