RT Journal Article SR Electronic T1 Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein JF Journal of Cell Science JO J. Cell Sci. FD The Company of Biologists Ltd SP 857 OP 864 DO 10.1242/jcs.075705 VO 124 IS 6 A1 Ibi, Miho A1 Zou, Peng A1 Inoko, Akihito A1 Shiromizu, Takashi A1 Matsuyama, Makoto A1 Hayashi, Yuko A1 Enomoto, Masato A1 Mori, Daisuke A1 Hirotsune, Shinji A1 Kiyono, Tohru A1 Tsukita, Sachiko A1 Goto, Hidemasa A1 Inagaki, Masaki YR 2011 UL http://jcs.biologists.org/content/124/6/857.abstract AB The keratin cytoskeleton performs several functions in epithelial cells and provides regulated interaction sites for scaffold proteins, including trichoplein. Previously, we found that trichoplein was localized on keratin intermediate filaments and desmosomes in well-differentiated, non-dividing epithelia. Here, we report that trichoplein is widely expressed and has a major function in the correct localization of the centrosomal protein ninein in epithelial and non-epithelial cells. Immunocytochemical analysis also revealed that this protein is concentrated at the subdistal to medial zone of both mother and daughter centrioles. Trichoplein binds the centrosomal proteins Odf2 and ninein, which are localized at the distal to subdistal ends of the mother centriole. Trichoplein depletion abolished the recruitment of ninein, but not Odf2, specifically at the subdistal end. However, Odf2 depletion inhibited the recruitment of trichoplein to a mother centriole, whereas ninein depletion did not. In addition, the depletion of each molecule impaired MT anchoring at the centrosome. These results suggest that trichoplein has a crucial role in MT-anchoring activity at the centrosome in proliferating cells, probably through its complex formation with Odf2 and ninein.