RT Journal Article
SR Electronic
T1 Trichoplein controls microtubule anchoring at the centrosome by binding to Odf2 and ninein
JF Journal of Cell Science
JO J. Cell Sci.
FD The Company of Biologists Ltd
SP 857
OP 864
DO 10.1242/jcs.075705
VO 124
IS 6
A1 Ibi, Miho
A1 Zou, Peng
A1 Inoko, Akihito
A1 Shiromizu, Takashi
A1 Matsuyama, Makoto
A1 Hayashi, Yuko
A1 Enomoto, Masato
A1 Mori, Daisuke
A1 Hirotsune, Shinji
A1 Kiyono, Tohru
A1 Tsukita, Sachiko
A1 Goto, Hidemasa
A1 Inagaki, Masaki
YR 2011
UL http://jcs.biologists.org/content/124/6/857.abstract
AB The keratin cytoskeleton performs several functions in epithelial cells and provides regulated interaction sites for scaffold proteins, including trichoplein. Previously, we found that trichoplein was localized on keratin intermediate filaments and desmosomes in well-differentiated, non-dividing epithelia. Here, we report that trichoplein is widely expressed and has a major function in the correct localization of the centrosomal protein ninein in epithelial and non-epithelial cells. Immunocytochemical analysis also revealed that this protein is concentrated at the subdistal to medial zone of both mother and daughter centrioles. Trichoplein binds the centrosomal proteins Odf2 and ninein, which are localized at the distal to subdistal ends of the mother centriole. Trichoplein depletion abolished the recruitment of ninein, but not Odf2, specifically at the subdistal end. However, Odf2 depletion inhibited the recruitment of trichoplein to a mother centriole, whereas ninein depletion did not. In addition, the depletion of each molecule impaired MT anchoring at the centrosome. These results suggest that trichoplein has a crucial role in MT-anchoring activity at the centrosome in proliferating cells, probably through its complex formation with Odf2 and ninein.