PT  - JOURNAL ARTICLE
AU  - Arquint, Christian
AU  - Sonnen, Katharina F.
AU  - Stierhof, York-Dieter
AU  - Nigg, Erich A.
TI  - Cell-cycle-regulated expression of STIL controls centriole number in human cells
AID  - 10.1242/jcs.099887
DP  - 2012 Mar 01
TA  - Journal of Cell Science
PG  - 1342--1352
VI  - 125
IP  - 5
4099  - http://jcs.biologists.org/content/125/5/1342.short
4100  - http://jcs.biologists.org/content/125/5/1342.full
SO  - J. Cell Sci.2012 Mar 01; 125
AB  - Control of centriole number is crucial for genome stability and ciliogenesis. Here, we characterize the role of human STIL, a protein that displays distant sequence similarity to the centriole duplication factors Ana2 in Drosophila and SAS-5 in Caenorhabditis elegans. Using RNA interference, we show that STIL is required for centriole duplication in human cells. Conversely, overexpression of STIL triggers the near-simultaneous formation of multiple daughter centrioles surrounding each mother, which is highly reminiscent of the phenotype produced by overexpression of the polo-like kinase PLK4 or the spindle assembly abnormal protein 6 homolog (SAS-6). We further show, by fluorescence and immunoelectron microscopy, that STIL is recruited to nascent daughter centrioles at the onset of centriole duplication and degraded, in an APC/CCdc20–Cdh1-dependent manner, upon passage through mitosis. We did not detect a stable complex between STIL and SAS-6, but the two proteins resemble each other with regard to both localization and cell cycle control of expression. Thus, STIL cooperates with SAS-6 and PLK4 in the control of centriole number and represents a key centriole duplication factor in human cells.