PT  - JOURNAL ARTICLE
AU  - Loukil, Abdelhalim
AU  - Zonca, Manuela
AU  - Rebouissou, Cosette
AU  - Baldin, Véronique
AU  - Coux, Olivier
AU  - Biard-Piechaczyk, Martine
AU  - Blanchard, Jean-Marie
AU  - Peter, Marion
TI  - High-resolution live-cell imaging reveals novel cyclin A2 degradation foci involving autophagy
AID  - 10.1242/jcs.139188
DP  - 2014 May 15
TA  - Journal of Cell Science
PG  - 2145--2150
VI  - 127
IP  - 10
4099  - http://jcs.biologists.org/content/127/10/2145.short
4100  - http://jcs.biologists.org/content/127/10/2145.full
SO  - J. Cell Sci.2014 May 15; 127
AB  - Cyclin A2 is a key player in the regulation of the cell cycle. Its degradation in mid-mitosis relies on the ubiquitin-proteasome system (UPS). Using high-resolution microscopic imaging, we find that cyclin A2 persists beyond metaphase. Indeed, we identify a novel cyclin-A2-containing compartment that forms dynamic foci. Förster (or fluorescence) resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) analyses show that cyclin A2 ubiquitylation takes place predominantly in these foci before spreading throughout the cell. Moreover, inhibition of autophagy in proliferating cells induces the stabilisation of a subset of cyclin A2, whereas induction of autophagy accelerates the degradation of cyclin A2, thus showing that autophagy is a novel regulator of cyclin A2 degradation.