PT  - JOURNAL ARTICLE
AU  - Amin, Mohammed Abdullahel
AU  - Itoh, Go
AU  - Iemura, Kenji
AU  - Ikeda, Masanori
AU  - Tanaka, Kozo
TI  - CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome alignment
AID  - 10.1242/jcs.150755
DP  - 2014 Jul 01
TA  - Journal of Cell Science
PG  - 2818--2824
VI  - 127
IP  - 13
4099  - http://jcs.biologists.org/content/127/13/2818.short
4100  - http://jcs.biologists.org/content/127/13/2818.full
SO  - J. Cell Sci.2014 Jul 01; 127
AB  - The cytoplasmic linker protein (CLIP)-170, an outer kinetochore protein, has a role in kinetochore–microtubule attachment and chromosome alignment during mitosis. However, the mechanism by which CLIP-170 is involved in chromosome alignment is not known. Here, we show that CLIP-170 colocalizes with Polo-like kinase 1 (PLK1) at kinetochores during early mitosis. Depletion of CLIP-170 results in a significant reduction in PLK1 recruitment to kinetochores and causes kinetochore-fiber (K-fiber) instability and defects in chromosome alignment at the metaphase plate. These phenotypes are dependent on the phosphorylation of CLIP-170 at a CDK1-dependent site, T287, as ectopic expression of wild-type CLIP-170, but not the expression of a non-phosphorylatable mutant, CLIP-170-T287A, restores PLK1 localization at kinetochores and rescues K-fiber stability and chromosome alignment in CLIP-170-depleted cells. These data suggest that CLIP-170 acts as a novel recruiter and spatial regulator of PLK1 at kinetochores during early mitosis, promoting K-fiber stability and chromosome alignment for error-free chromosome segregation.