RT Journal Article
SR Electronic
T1 CLIP-170 recruits PLK1 to kinetochores during early mitosis for chromosome alignment
JF Journal of Cell Science
JO J. Cell Sci.
FD The Company of Biologists Ltd
SP 2818
OP 2824
DO 10.1242/jcs.150755
VO 127
IS 13
A1 Amin, Mohammed Abdullahel
A1 Itoh, Go
A1 Iemura, Kenji
A1 Ikeda, Masanori
A1 Tanaka, Kozo
YR 2014
UL http://jcs.biologists.org/content/127/13/2818.abstract
AB The cytoplasmic linker protein (CLIP)-170, an outer kinetochore protein, has a role in kinetochore–microtubule attachment and chromosome alignment during mitosis. However, the mechanism by which CLIP-170 is involved in chromosome alignment is not known. Here, we show that CLIP-170 colocalizes with Polo-like kinase 1 (PLK1) at kinetochores during early mitosis. Depletion of CLIP-170 results in a significant reduction in PLK1 recruitment to kinetochores and causes kinetochore-fiber (K-fiber) instability and defects in chromosome alignment at the metaphase plate. These phenotypes are dependent on the phosphorylation of CLIP-170 at a CDK1-dependent site, T287, as ectopic expression of wild-type CLIP-170, but not the expression of a non-phosphorylatable mutant, CLIP-170-T287A, restores PLK1 localization at kinetochores and rescues K-fiber stability and chromosome alignment in CLIP-170-depleted cells. These data suggest that CLIP-170 acts as a novel recruiter and spatial regulator of PLK1 at kinetochores during early mitosis, promoting K-fiber stability and chromosome alignment for error-free chromosome segregation.