PT  - JOURNAL ARTICLE
AU  - Löschberger, Anna
AU  - Franke, Christian
AU  - Krohne, Georg
AU  - van de Linde, Sebastian
AU  - Sauer, Markus
TI  - Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution
AID  - 10.1242/jcs.156620
DP  - 2014 Oct 15
TA  - Journal of Cell Science
PG  - 4351--4355
VI  - 127
IP  - 20
4099  - http://jcs.biologists.org/content/127/20/4351.short
4100  - http://jcs.biologists.org/content/127/20/4351.full
SO  - J. Cell Sci.2014 Oct 15; 127
AB  - Here, we combine super-resolution fluorescence localization microscopy with scanning electron microscopy to map the position of proteins of nuclear pore complexes in isolated Xenopus laevis oocyte nuclear envelopes with molecular resolution in both imaging modes. We use the periodic molecular structure of the nuclear pore complex to superimpose direct stochastic optical reconstruction microscopy images with a precision of <20 nm on electron micrographs. The correlative images demonstrate quantitative molecular labeling and localization of nuclear pore complex proteins by standard immunocytochemistry with primary and secondary antibodies and reveal that the nuclear pore complex is composed of eight gp210 (also known as NUP210) protein homodimers. In addition, we find subpopulations of nuclear pore complexes with ninefold symmetry, which are found occasionally among the more typical eightfold symmetrical structures.