PT - JOURNAL ARTICLE AU - Löschberger, Anna AU - Franke, Christian AU - Krohne, Georg AU - van de Linde, Sebastian AU - Sauer, Markus TI - Correlative super-resolution fluorescence and electron microscopy of the nuclear pore complex with molecular resolution AID - 10.1242/jcs.156620 DP - 2014 Oct 15 TA - Journal of Cell Science PG - 4351--4355 VI - 127 IP - 20 4099 - http://jcs.biologists.org/content/127/20/4351.short 4100 - http://jcs.biologists.org/content/127/20/4351.full SO - J. Cell Sci.2014 Oct 15; 127 AB - Here, we combine super-resolution fluorescence localization microscopy with scanning electron microscopy to map the position of proteins of nuclear pore complexes in isolated Xenopus laevis oocyte nuclear envelopes with molecular resolution in both imaging modes. We use the periodic molecular structure of the nuclear pore complex to superimpose direct stochastic optical reconstruction microscopy images with a precision of <20 nm on electron micrographs. The correlative images demonstrate quantitative molecular labeling and localization of nuclear pore complex proteins by standard immunocytochemistry with primary and secondary antibodies and reveal that the nuclear pore complex is composed of eight gp210 (also known as NUP210) protein homodimers. In addition, we find subpopulations of nuclear pore complexes with ninefold symmetry, which are found occasionally among the more typical eightfold symmetrical structures.