Oligonucleotides used in this study
| delRHO5-5 | 5′-CGC CGA AAT ATT TAT TAA ATA CAT ATA GTA TAC TAA TAA GTC TGC TTC GTA CGC TGC AGG TCG AC-3′ |
| delRHO5-3 | 5′-CGC CGA AAT ATT TAT TAA ATA CAT ATA GTA TAC TAA TAA GTC TGC TTC GTA CGC TGC AGG TCG AC-3′ |
| RHO5-5neu | 5′-CCA CAT CGG TAA GTG ATG ACC-3′ |
| RHO5-3neu | 5′-CCA TTA CTA TGG TTC AAC CTG G-3′ |
| RHO5Q91Hneu | 5′-CAT AAC GGT CTT AAA CGA TCG TAA TCT TCG TGT CCT GCG GTG TCC CAT AAA TTG-3′ |
| RHO5Bam | 5′-GGA TGG ATC CAT GAG GTC TAT TAA ATG TGT G-3′ |
| RHO5-3¢Sph | 5′-GGA TGC ATG CTT GGA AAA GAA AGT CGC GG-3′ |
| delBEM2-5 | 5′-GGA TTG TAT ACA TTT ACC ACG AAA ATT GTT TAT TGC TTG AAA TAATCC ATA GGC CAC TAG TGG ATC TG-3′ |
| delBEM2-3 | 5′-GGA CAA TTC ATC TCT TTT CCT GCG GTT ATG AGC GAA ACC AAC GTT GCT TCG TAC GCT GCA GGT CGA C-3′ |
| GAL1atg | 5′-GGC GAA TTC TAT AGT TTT TTC TCC TTG ACG-3′ |
| GAL10atg | 5′-CTC GGA TCC TTA TAT TGA ATT TTC-3′ |
| M13 reverse | 5′-CAG GAA ACA GCT ATG ACC ATG-3′ |
Restriction sites used for subcloning are underlined.