Table 2.

Oligonucleotides used in this study

delRHO5-55′-CGC CGA AAT ATT TAT TAA ATA CAT ATA GTA TAC TAA TAA GTC TGC TTC GTA CGC TGC AGG TCG AC-3′
delRHO5-35′-CGC CGA AAT ATT TAT TAA ATA CAT ATA GTA TAC TAA TAA GTC TGC TTC GTA CGC TGC AGG TCG AC-3′
RHO5-5neu5′-CCA CAT CGG TAA GTG ATG ACC-3′
RHO5-3neu5′-CCA TTA CTA TGG TTC AAC CTG G-3′
RHO5Q91Hneu5′-CAT AAC GGT CTT AAA CGA TCG TAA TCT TCG TGT CCT GCG GTG TCC CAT AAA TTG-3′
RHO5Bam5′-GGA TGG ATC CAT GAG GTC TAT TAA ATG TGT G-3′
RHO5-3¢Sph5′-GGA TGC ATG CTT GGA AAA GAA AGT CGC GG-3′
delBEM2-55′-GGA TTG TAT ACA TTT ACC ACG AAA ATT GTT TAT TGC TTG AAA TAATCC ATA GGC CAC TAG TGG ATC TG-3′
delBEM2-35′-GGA CAA TTC ATC TCT TTT CCT GCG GTT ATG AGC GAA ACC AAC GTT GCT TCG TAC GCT GCA GGT CGA C-3′
GAL1atg5′-GGC GAA TTC TAT AGT TTT TTC TCC TTG ACG-3′
GAL10atg5′-CTC GGA TCC TTA TAT TGA ATT TTC-3′
M13 reverse5′-CAG GAA ACA GCT ATG ACC ATG-3′
  • Restriction sites used for subcloning are underlined.