Table 4.

Indirect determination of Slt2p MAP-kinase activity in a rho5 deletion background

Growth temperatureWildtypeΔrho5
25°C0.7±0.32.3±1.2
37°C8.4±5.350.5±22.7
  • The activity of the Slt2p MAP-kinase cascade was determined using the plasmid-based reporter system described previously ( Kirchrath et al., 2000); for details, see Materials and Methods. The mean and standard deviation values are shown for four different transformants.