Table 1.

Strains used in this study

StrainGenotypeSource
SEY6210 MAT α leu2-3, 112 ura3-52 his3-Δ20 trp1-Δ901 lys2-801 suc2-Δ Robinson et al., 1988
JCY300SEY6210; vps30Δ::HIS3 Seaman et al., 1997
PBY58SEY6210; vps38Δ::HIS3This study
PBY51SEY6210; vps30Δ::HIS3, vps38Δ::HIS3This study
EMY18SEY6210; vps35Δ::HIS3 Seaman et al., 1997
BHY152SEY6210; vps5Δ::HIS3 Horazdovsky et al., 1997
KKY11BHY11; vps17Δ::HIS3 Kohrer and Emr, 1993
PBY142SEY6210; vps5Δ::HIS3, vps17Δ::HIS3This study
TVY1SEY6210; pep4Δ::LEU2Emr Lab Strain Collection
PBY139SEY6210; pep4Δ::LEU2, vps30Δ::HIS3This study
PBY140SEY6210; pep4Δ::LEU2, vps38Δ::HIS3This study
EMY30SEY6210; pep4Δ::LEU2, vps35Δ::HIS3Emr Lab Strain Collection
PHY102SEY6210; vps34Δ::TRP1 Herman and Emr, 1990
vps34tsf SEY6210; vps34Δ::TRP1; pRS416,vps34tsf Stack et al., 1995
PBY34SEY6210; VPS10-GFP:TRP1This study
PBY35SEY6210; KEX2-GFP:TRP1This study
PBY70SEY6210; VPS17-GFP:TRP1This study
PBY82SEY6210; KEX2-HA:TRP1This study
PBY76SEY6210; VPS10-HA:TRP1This study
PBY86SEY6210; VPS5-GFP:TRP1This study
PBY38SEY6210; VPS10-GFP:TRP1, Δvps30This study
PBY40SEY6210; VPS10-GFP:TRP1, Δvps38This study
PBY39SEY6210; KEX2-GFP:TRP1 Δvps30This study
PBY30SEY6210; KEX2-GFP:TRP1 Δvps38This study
PBY77SEY6210; VPS10-HA:TRP1 Δvps38This study
PBY124SEY6210; Vps10-GFP:TRP1, vps34tsfThis study
PBY123SEY6210; Vps17-GFP:TRP1, vps34tsfThis study
PBY88SEY6210; Vps5-GFP:TRP1, Δvps34::TRP1This study
PBY87SEY6210; Vps5-GFP:TRP1, Δvps38::HIS3This study
PBY111SEY6210; Vps17-GFP:TRP1, Δvps34::TRP1This study
PBY112SEY6210; Vps17-GFP:TRP1, Δvps38::HIS3This study
PBY142SEY6210; Vps10-GFP, Δvps5::HIS3This study
PBY116SEY6210; Vps17-GFP:TRP1, Δfab1::HIS3This study
PBY117SEY6210; Vps17-GFP, Δmss4::HIS3; pYCplacIII.mss4-102This study
PBY115SEY6210; Vps17-GFP:TRP1, Δstt44::HIS3; pRS415.stt4-4This study
PBY114SEY6210; Vps17-GFP:TRP1, Δpik1::HIS3; pRS413.pik1-83This study