Table 2.

Expression of dominant active Rho3Q71L caused a defect in cytokinesis

Formation of F-actin ring (%) Number of nuclei (%)
NormalAbnormalMis-positioned1 or 2 (with F-actin ring)2 (without F-actin ring)>20
Control10000100000
Rho3Q71L29.438.632.067.828.00.83.4 *
  • Wild-type cells containing pREP1 (control) or pREP1rho3Q71L were grown in the absence of thiamine for 20 hours at 30°C. About 150 mitotic cells or 500 cells were counted for the observation of the F-actin ring or the nucleus, respectively.

  • * All of these cells were mini cells.