Table 1.

S. pombe strains used in this study

StrainGenotypeSource
HM123 h- leu1-32 Laboratory stock
428h h- ade6-M210 leu1-32 ura4-D18 Laboratory stock
429h h+ ade6-M216 leu1-32 ura4-D18 Laboratory stock
rad3Δ h- rad3::ura4+ ade6-704 leu1-32 ura4-D18 A. M. Carr
pds5Δ h+ pds5::ura4+ ade6-M210 leu1-32 ura4-D18 This study
h- pds5::ura4+ ade6-M216 leu1-32 ura4-D18 This study
pds5Δ (LEU2) h- pds5::LEU2 ade6-M216 leu1-32 ura4-D18 This study
HM248 h- his2 ade6-M210 (Ch16 ade6-M216) T. Toda
pds5Δ Ch16 h- pds5::ura4+ ade6-M210 ura4-D18 (Ch16 ade6-M216) This study
pds5-GFP h- pds5-GFP:kanMX6 leu1-32 ura4-D18 This study
pds5-HA h- pds5-HA:kanMX6 leu1-32 ura4-D18 This study
nda3-KM311 h+ nda3-KM311 leu1-32 ura4-D18 his2 T. Toda
nda3-KM311 pds5Δ h+ nda3-KM311 pds5::ura4+ leu1-32 ura4-D18 This study
ndc80-GFP h- ndc80-GFP:kanMX6 leu1-32 ura4-D18 J. V. Kilmartin
nda3-KM311 ndc80-GFP pds5Δ h+ nda3-KM311 pds5::ura4 ndc80-GFP:kanMX6 leu1-32 ura4-D18 This study
rad21-K1 h- rad21-K1:ura4+ ade6-M216 leu1-32 ura4-D18 his7-366 H. Ikeda
rad21-K1 pds5-GFP h- rad21-K1:ura4+ pds5-GFP:kanMX6 ade6-M216 leu1-32 ura4-D18 his7-366 This study
rad21-HA h- rad21-HA:LEU2 leu1-32 M. Yanagida
rad21-GFP h- rad21-GFP:LEU2 leu1-32 M. Yanagida
pds5Δ rad21-GFP h- rad21-GFP:LEU2 pds5::ura4+ leu1-32 ura4-D18 This study
rad21-K1 pds5Δ h+ h- rad21-K1:ura4/rad21+ pds5::LEU2/pds5+ ade6-M210/ade6-M216 leu1-32/leu1-32 ura4-D18/ura4-D18 This study
mis4-242 h- mis4-242 leu1-32 M. Yanagida
pds5Δ mis4-242 h- mis4-242 pds5::LEU2 leu1-32 This study
bub1Δ h- bub1::LEU2 ade6-M210 leu1-32 ura4-DS/E his1-102 J.-P. Javerzat
pds5Δ bub1Δ h- pds5::ura4+ bub1::LEU2 ade6-M210 leu1-32 ura4-D18/DS/E This study
GFP-swi6h- ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18R. Allshire
GFP-swi6 pds5Δ h- pds5::ura4+ ars1(MluI)::pREP81Xgfpswi6-LEU2 ade6-M210 leu1-32 ura4-D18 This study