Table 4.

Nocodazole sensitivity of γ-tubulin areas in CV-1 cells

Cell cycle stageInterphaseProphasePrometaphase/pseudo-prometaphase
TreatmentDMSONocodazole (30 min)Nocodazole (12 h)DMSONocodazole (30 min)Nocodazole (12 h)DMSONocodazole (30 min)Nocodazole (12 h)
γ-Tubulin area (μm2)0.48±0.14 (n=34)0.48±0.11 (n=20)0.52±0.096 (n=49)1.44±0.56 (n=52)0.68±0.28 (n=36)0.52±0.14 (n=58)3.4±2.4 (n=62)0.92±0.18 (n=69)0.64±0.14 (n=67)
Nocodazole was added to the culture medium from a stock solution dissolved in DMSO. Final concentrations were 20 μM nocodazole, 0.1% DMSO. Control cultures were treated with only 0.1% DMSO.