Table 1.

Analysis of peripherin motility

Time in DMCell domainParticle/squiggleNumber countedTime moving (%)Time pausing (%)Antero movements (%)Retro movements (%)Reversals (%)Average anterograde motility (μm/sec)Range anterograde (μm/sec)Average retrograde motility (μm/sec)Range retrograde (μm/sec)
PC12 cell 0.5-6 hours DMCell bodyParticle5046547525460.34±0.210.08-1.450.34±0.240.08-1.20
PC12 cell 0.5-6 hours DMCell bodySquiggle6468325941200.40±0.250.08-1.140.38±0.280.08-1.22
PC12 cell 24-72 hours DMCell bodyParticle5067335545500.31±0.200.08-1.110.30±0.220.08-1.14
PC12 cell 24-72 hours DMCell bodySquiggle5063376238340.41±0.240.08-1.090.34±0.230.08-1.25
PC12 cell 24-72 hours DMNeuriteParticle777525653580.33±0.240.08-1.450.30±0.200.08-1.54
PC12 cell 24-72 hours DMNeuriteSquiggle507030623860.31±0.290.08-1.210.30±0.280.08-1.00
PC12 cell 24-72 hours DMGrowth coneParticle5053475347500.35±0.250.08-1.190.32±0.190.08-0.92
PC12 cell 24-72 hours DMGrowth coneSquiggle5258425149480.32±0.180.08-1.050.30±0.220.08-1.21
BHK-21 Fibroblast*Cell bodyParticle5345556634360.39±0.240.08-1.650.43±0.260.08-1.40
  • Time-lapse images of GFP-peripherin particles and squiggles in PC12 cells plated in DM for either 0.5-6 hours or 24-72 hours were captured every 5 seconds. A large photobleach zone was made in experiments involving analyses of peripherin motility in neurites (24-72 hours in DM). A pause was defined as no movement or <0.5 μm in 6 seconds. A reversal of movement was defined as an individual particle or squiggle that moved first in one direction and then in the opposite direction. For comparison, the movements of GFP-vimentin particles in well-spread BHK-21 fibroblasts were analyzed in peripheral regions.

  • * BHK-21 cells were grown as described in Materials and Methods.