Table 1.

Yeast strains

StrainGenotype
PN22 h- leu1-32
PN35 h+ leu1-32 cdc25-22
S145 h- leu1-32 wee1-50
S176 h+ leu1-32 ade6-M210 cdc2-3w
S778 h+ leu1-32 ade6-M216
S781 h- leu1-32 ade6-M210
S898 h+ leu1-32 ura4-259 cdc25::ura4+ cdc2-3w
S964 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32
S1285 h+ his5-303
S1291 h+ ade6-M216 leu1-32 mek1::kanMX6
S1292 h- ade6-M210 leu1-32 mek1::kanMX6
S1293 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 mek1::kanMX6/mek1::kanMX6
S1294 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 mek1-3HA-kanMX6/mek1-3HA-kanMX6
S1295 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 ura4-d18/ura4-d18 meu13::ura4+/meu13::ura4+
S1296 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 ura4-d18/ura4-d18 meu13::ura4+/meu13::ura4+ mek1::kanMX6/mek1::kanMX6
S1297 h- leu1-32 ade6-M210 nmt1(41X)-mek1-GFP::leu1+
S1298 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 nmt1(41X)-mek1-GFP::leu1+/leu1-32
S1299 h+ leu1-32 cdc25-9A
S1300 h- leu1-32 mek1::kanMX6
S1301 h+ his5-303 mek1::kanMX6
S1302 h+ leu1-32 ade6-M210 nmt1(41X)-mek1-GFP::leu1+ cdc25-9A
S1306 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 meu13::kanMX6+/meu13::kanMX6+
S1307 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 meu13::kanMX6+/meu13::kanMX6 cdc25-9A/cdc25-9A
S1308 h-/h- pat1-114/pat1-114 ade6-M216/ade6-M210 leu1-32/leu1-32 cdc25-9A/cdc25-9A